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Fumie MAGATA Yoshikazu ISHIDA Akio MIYAMOTO Hidefumi FURUOKA Hisashi INOKUMA Takashi SHIMIZU 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(1):81-84
We investigated the
concentration of the bacterial endotoxin lipopolysaccharide (LPS) in the blood, ovarian
follicular fluid and uterine fluid of a clinical case of bovine metritis. A 2-year-old
lactating Holstein cow exhibited continuous fever >39.5°C for more than 2 weeks after
normal calving. The cow produced a fetid, watery, red-brown uterine discharge from the
vagina and was diagnosed with metritis. The LPS concentrations in plasma and uterine fluid
were 0.94 and 6.34 endotoxin units (EU)/ml, respectively. One of seven
follicles showed an extremely high level of LPS (12.40 EU/ml) compared to
the other follicles (0.62–0.97 EU/ml). These results might suggest the
presence of high concentration of LPS in follicles in cows with postpartum metritis. 相似文献
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Rina KIHARA Yoshinori KASASHIMA Katsuhiko ARAI Yasunori MIYAMOTO 《Journal of Equine Science》2011,22(3):57-60
Injury initiates a repair process characterized by influx of fibroblasts and the rapid
formation of fibrous scar tissue and subsequent tissue contraction. The response to injury
and behavior of the different tendon fibroblast populations, however, has been poorly
characterized. We hypothesized that the fibroblasts recovered from tendon with acute
injury would exhibit different cell properties relating to adhesion, migration and
tensegrity. To test this hypothesis we evaluated the ability of fibroblasts recovered from
normal and injured equine superficial digital flexor tendons (SDFTs). The injured
tendon-derived cells showed greater contraction of the collagen gel but poorer adhesion to
pepsin-digested collagen, and migration over extracellular matrix proteins compared to
normal SDFT-derived fibroblasts. Thus, the cells present within the tendon after injury
display different behavior related to wound healing. 相似文献
4.
WULIJIDELIGEN Takayuki ASAHINA Kazushi HARA Kensuke ARAKAWA Hiroyuki NAKANO Taku MIYAMOTO 《Animal Science Journal》2012,83(10):704-711
The purification and characterization of a bacteriocin produced by Leuconostoc mesenteroides strain 406 that was isolated from traditional Mongolian fermented mare's milk, airag, were carried out. Leuconostoc mesenteroides strain 406 was identified on the basis of its morphological and biochemical characteristics and carbohydrate fermentation profile and by API 50 CH kit and 16S ribosomal DNA analyses. The neutral‐pH cell‐free supernatant of this bacterium inhibited the growth of several lactic acid bacteria and food spoilage and pathogenic organisms, including Listeria monocytogenes and Clostridium botulinum. The bacteriocin was heat‐stable and not sensitive to acid and alkaline conditions, but was sensitive to several proteolytic enzymes such as pepsin, pronase E, proteinase K, trypsin, and α‐chymotrypsin, but not catalase. Optimum bacteriocin production (4000 activity units/mL) was achieved when the strain was cultured at 25°C for 24–36 h in Man Rogosa Sharpe medium. The bacteriocin was partially purified by ammonium sulfate precipitation (80% saturation), dialysis (cut‐off MW: 1000), and gel filtration chromatography. Sodium dodecyl sulfate‐polyacrylamide gel electrophoresis revealed that the bacteriocin had a molecular weight of approximately 3.3 kDa. To our knowledge, this is the first report of the isolation of a bacteriocin‐producing Leuconostoc strain from airag. An application to fermented milks would be desired. 相似文献
5.
Rasoul KOWSAR Nina HAMBRUCH Jinghui LIU Takashi SHIMIZU Christiane PFARRER Akio MIYAMOTO 《The Journal of reproduction and development》2013,59(5):470-478
This study aimed to investigate the role of epithelial cells in regulating innate
immunity in bovine oviduct epithelial cell (BOEC) culture. We studied the effect of
Escherichia coli lipopolysaccharide (LPS) and its interaction with
ovarian steroids, estradiol (E2) and progesterone (P4), and luteinizing hormone (LH) at
concentrations observed during the preovulatory period on immune responses in BOEC
culture. Immunohistochemistry of oviduct tissue showed intensive expression of Toll-like
receptor-4 (TLR-4) and TLR-2 in epithelial cells. A dose of 10 ng/ml LPS stimulated
TLR-4, cyclooxygenase-2 (COX-2), nuclear factor kappa
B inhibitor A (NFKBIA), interleukin 1β (IL-1β) and tumor
necrosis factor α (TNF-α) expression, indicating an early
pro-inflammatory response. A dose of 100 ng/ml LPS did not induce expression of these
genes but stimulated TLR-2, IL-10,IL-4
and microsomal prostaglandin E synthase-1 (mPGES-1) expression and PGE2
secretion, indicating an anti-inflammatory response. Ovarian steroids and LH completely
block LPS (10 ng/ml)-induced TLR-4, IL-1β and
TNF-α expression as well as LPS (100 ng/ml)-induced
TLR-2 expression. Taken together, this study suggests the existence of
an early signaling system to respond to infection in the BOEC. In addition, ovarian
steroids and LH may play a critical role in inducing homeostasis and in controlling
hyperactive pro-inflammatory responses detrimental to epithelial cells, sperm and the
embryo. 相似文献
6.
Matsubayashi M Carreno RA Tani H Yoshiuchi R Kanai T Kimata I Uni S Furuya M Sasai K 《Veterinary parasitology》2011,176(2-3):270-274
Cystoisospora spp. from feces in dogs, cats, and raccoon dogs were isolated, sequenced at the small subunit ribosomal RNA gene locus and compared to other Cystoisospora spp. Cystoisospora oocysts from dogs and raccoon dogs were morphologically similar with those of C. ohioensis, and cat isolates were similar with those of C. felis. The sequences from dogs and raccoon dogs, and cats have a homology with C. ohioensis and C. felis, respectively. Phylogenetic analysis of the DNA sequences showed that the dog and raccoon dog isolates were nested in a clade with other Cystoisospora spp. including C. ohioensis, C. belli, and C. orlovi. The cat isolate formed a sister group with C. felis that was a separate clade from the dog and raccoon dog group. We report sequence variation in these Cystoisospora sequences and have identified raccoon dogs as another carnivore host for Cystoisospora spp. infecting dogs. 相似文献
7.
The purpose of this study was to evaluate the survival and colonization of Lactobacillus plantarum 301102 in porcine gastrointestinal tract and its influence on the intestinal microflora. This strain was isolated from traditional cheese from Inner Mongolia, China. Fermented milk prepared with strain 301102 was administered to pigs and fecal microflora was examined. Intestinal passage of strain 301102 was monitored by a combination of selection with selective medium and the carbohydrate fermentation test, and confirmed by analysis of plasmid DNA patterns. Colonization of this strain was assessed by recovery 7 days after administration. The numbers of Lactobacillus and Bifidobacterium cells in the feces of pigs administered fermented milk prepared with strain 301102 were increased. This strain was recovered from all the pigs during the administration period, and from four of six pigs 7 days after administration. L. plantarum 301102 can pass through the gastrointestinal tract, balance the intestinal microflora, and colonize the intestinal tract, and is therefore an appropriate candidate for a probiotic. 相似文献
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D. UENO A. YABUKI T. OBI M. SHIRAISHI A. NISHIO & A. MIYAMOTO 《Journal of veterinary pharmacology and therapeutics》2009,32(3):264-270
We investigated the effect of bradykinin (BK) on isolated equine basilar arterial rings with and without endothelium. BK induced concentration-dependent contraction of resting arterial rings and no relaxation when the rings were precontracted by prostaglandin F2α . The maximal response and pD2 value were 161.2 ± 28.1% (to 60 m m KCl-induced contraction) and 8.24 ± 0.25 respectively. The cumulative concentration–response curve for BK was not shifted to the right by des-Arg9 -[Leu8 ]-BK (a B1 -receptor antagonist), HOE140 (a B2 -receptor antagonist) or NPC567 (another B2 -receptor antagonist). In four of six basilar arteries, NPC567 induced concentration-dependent contraction. Indomethacin (a cyclooxygenase inhibitor), nordihydroguaiaretic acid (a lipoxygenase inhibitor), quinacrine (a phospholipase A2 inhibitor), tetrodotoxin (a selective blocker of Na+ channels), guanethidine (a nor-adrenergic neuron blocking drug), phentolamine (an α-adrenoceptor antagonist), Nω-nitro- l -arginine ( l -NNA, a nitric oxide (NO) synthase inhibitor) and endothelial denudation did not affect the BK-induced contraction. l -NNA and indomethacin induced contraction and relaxation under resting vascular tone respectively. These results suggest that endothelial cells are not involved in BK-induced contraction and that the contraction is not mediated via activation of known B1 and B2 receptors. Arachidonic acid metabolites and neurotransmitters like norepinephrine and NO might not play a role in BK-induced contraction in equine basilar artery. 相似文献
10.
Motoki SASAKI Yoko AMANO Daisuke HAYAKAWA Toshio TSUBOTA Hajime ISHIKAWA Toshihiro MOGOE Seiji OHSUMI Masafumi TETSUKA Akio MIYAMOTO Yutaka FUKUI Teguh BUDIPITOJO Nobuo KITAMURA 《The Journal of reproduction and development》2014,60(1):62-67
In this study, we examined the existence and structure of areolae and the
steroidogenesis of areolar trophoblast cells in the Antarctic minke whale placenta
morphologically and immunohistochemically. Placentas were collected from the 15th, 16th
and 18th Japanese Whale Research Program under Special Permit in the Antarctic (JARPA) and
1st JARPA II organized by the Institute of Cetacean Research in Tokyo, Japan. The opening
and cavity of fetal areolae formed by taller columnar trophoblast cells (areolar
trophoblast cells) with long microvilli and a bright cytoplasm, as compared with the
trophoblast cells of the chorionic villi interdigitating with the endometrial crypts, were
recognized in observations of serial sections. The opening of the areolar cavity was
hidden by chorionic villi with areolar trophoblast cells. Furthermore, a closed pouch-like
structure lined by tall columnar cells similar to areolar trophoblast cells within the
stroma of chorionic villi was noticed and continued to the areolar cavity, with the
opening seen on serial sections. In a surface investigation of the chorion and endometrium
by SEM, maternal (endometrial) areolae irregularly surrounded by endometrial folds were
obvious. Moreover, we distinguished areolar trophoblast cells with long microvilli
attached with many blebs from trophoblast cells. In our immunohistochemical observations,
a steroidogenic enzyme, cytochrome P450 side chain cleavage enzyme (P450scc), was detected
with strong immunoreactivity in trophoblast cells. However, areolar trophoblast cells
showed weak or no immunoreactivity for P450scc. 相似文献