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排序方式： 共有71条查询结果，搜索用时 15 毫秒
1.
Yuval Ramot Michal Steiner Netanel Amouyal Yossi Lavie Guy Klaiman Abraham J. Domb Abraham Nyska Tal Hagigit 《Journal of toxicologic pathology》2021,34(1):11
Fracture-related infections remain a leading cause of morbidity and mortality. We aimed to establish a simple contaminated radial osteotomy model to assess the efficacy of a biodegradable polymer poly(sebacic-co-ricinoleic acid) [p(SA-RA)] containing 20% w/w gentamicin. A unilateral transverse osteotomy was induced in Sprague-Dawley (SD) rats, followed by application of Staphylococcus aureus suspension over the fracture. After successfully establishing the contaminated open fracture model, we treated the rats either systemically (intraperitoneal cefuroxime), locally with p(SA-RA) containing gentamicin, or both. Control groups included non-contaminated group and contaminated groups that were either untreated or treated with the polymer alone. After 4 weeks, the bones were subjected to micro-CT scanning and microbiological and histopathology evaluations. Micro-CT analysis revealed similar changes in the group subjected to both local and systemic treatment as in the non-contaminated control group. Lack of detectable bacterial growth was noted in most animals of the group subjected to both local and systemic treatment, and all samples were negative for S. aureus. Histopathological evaluation revealed that all treatment modalities containing antibiotics were highly effective in reducing infection and promoting callus repair, resulting in early bone healing. While p(SA-RA) containing gentamicin treatment showed better results than cefuroxime, the combination of local and systemic treatment displayed the highest therapeutic potential in this model. 相似文献
2.
3.
The effects of γ-radiation on the immature stages ofLiriomyza trifolii Burgess were studied with doses of 2000-25 Gy. The high doses were lethal to all stages. At doses of 750 Gy or less, larvae
survived to pupate, but their number was reduced and the majority were either dead or deformed and did not give rise to adults.
Eggs and prepupae were more susceptible to radiation than other stages. Larval radiosensitivity decreased with progressing
instar, and pupal sensitivity decreased with increasing age.
Contribution from the Agricultural Research Organization. No. 2922-E, 1990 series. 相似文献
4.
A. HüTtermann Marie-Therese Oelbe E. Fritz G. Schlechte A. Bytom A. D. Jungk Y. Okon E. Fallik Rivka Hadas S. Sarig E. Yahalom Sara Tal A. Hartmann B. Kishinevsky Debora Gurfel Rina Lobel Y. Kapulnik D. A. Phillips Y. Hadar Y. Chen E. Jurkevitch J. Katan A. Gamliel H. Ziegler I. Chet A. Ordentlich Hadar Kless A. Oppenheim Avia Zilberstein D. Holland J. Berman A. Zamir R. Kaufman N. Bawnik C. Koncz J. Schell 《Phytoparasitica》1987,15(2):141-147
5.
Insect viruses can be exploited for the biological control of insect pests and serve as well as vehicles for the introduction
of ‘foreign genes’ into insect cells and insects. The present work describes some of the molecular events that take place
during the replication of theGalleria mellonella densonucleosis virus in the insect host. Viral RNA and protein synthesis were monitored. An understanding of the virus biology
is essential for further implementation of these viruses for biological control purposes. 相似文献
6.
Steinman A Zimmerman T Klement E Lensky IM Berlin D Gottlieb Y Baneth G 《Veterinary parasitology》2012,187(3-4):558-562
The prevalence of Theileria equi infection as well as the environmental and demographic risk factors for infection was studied in 590 healthy horses from 46 farms in Israel. The prevalence of T. equi DNA was assessed using a polymerase chain reaction for a segment of the Theileria 18S rRNA gene. The overall prevalence was 26.4% (156/590). There was a significant geographical variation in the prevalence of T. equi infection, ranging from 9.3% (25/270) in the central lowlands to 81.7% (49/60) in the Golan Heights. The prevalence of T. equi infection was found to be significantly associated with management types with more horses with access to pasture being positive. Breed was identified as a risk factor for T. equi infection in a univariate analysis with relatively high infection rates in the Quarter horse and local breeds (41.1% and 36.3% respectively), while ponies and Arabian horses had a relatively low prevalence (10% and 9.1%, respectively). However, since a correlation between geographic location and breed was found, it is difficult to draw definite conclusions regarding this risk factor. Age and gender were not found as risk factors for T. equi infection in this study. The environmental variables that were significantly associated with positivity were relative humidity and minimum land surface temperature at day which both showed negative correlation with T. equi prevalence. In conclusion, Israel was found to be enzootic for T. equi infection, as indicated by the high sub-clinical infection rate, which differed between geographical areas. 相似文献
7.
T Brevini G Pennarossa S Maffei F Gandolfi 《Reproduction in domestic animals = Zuchthygiene》2012,47(Z4):86-91
Huge amounts of work have been dedicated to the establishment of embryonic stem cell lines from farm animal species since the successful isolation of embryonic stem cells from the mouse and from the human. However, no conclusive results have been obtained so far, and validated lines have yet to be established in domestic animals. Many limiting factors have been suggested and need to be studied further to isolate truly pluripotent cell lines from livestock. In this review, we will discuss the difficulties in deriving and maintaining embryonic stem cell lines from farm animal embryos and how can this lack of success be explained. We will summarize results obtained in our laboratory regarding derivation of pluripotent cells in the pigs. Problems related to the identification of standard methods for derivation, maintenance and characterization of cell lines will also be examined. We will focus our attention on the need for appropriate stemness-related marker molecules that can be used to reliably investigate pluripotency in domestic species. Finally, we will review data presently available on functional key pluripotency-maintaining pathways in farm animals. 相似文献
8.
The relationships between the development of disease symptoms ofPuccinia sorghi, Exserohilum turcicum andHelminthosporium maydis on susceptible sweet corn plants, and the water status of the plants, were studied. At a relative water content (RWC) of 75% the development of sori ofP. sorghi, and the leaf area infected byE. turcicum andH. maydis, were inhibited by 38, 75 and 84%, respectively, as compared with the control plants (RWC = 100%).P. sorghi andH. maydis were not inhibited by a slight decrease in the RWC (96%), but development of leaf area infected byE. turcicum was inhibited by 33%.P. sorghi seemed to be better adapted to relatively high water deficiency in the host plants thanH. maydis orE. turcicum. 相似文献
9.
Vladislav Dubinin Tal Svoray Michael Dorman Avi Perevolotsky 《Landscape Ecology》2018,33(10):1815-1830
Context
Habitats characterized by improved soil moisture availability can function as microrefugia (hereafter referred to as “refugia”) for the persistence of rare plant species in dry environments. Such areas are dominated by Mediterranean woody vegetation (shrubland and woodland). An analysis of these refugia elucidates their spatial distribution at the landscape scale.Objectives
Explore whether potential refugia, detected using the upper quantile of the normalized difference vegetation index (NDVI), are related, in space and time, with the survivability of rare species in dry environments.Methods
We used upper NDVI quantile (25%) values to predict potential refugia in nine selected areas in northern parts of Israel from 1992 to 2011. Next, we developed an index based on the ratio of density (number of observations per area) of rare species in non-refugia versus refugia patches, per site (density of rare species index, DRSI). Finally, we examined the temporal stability of the DRSI using ANOVA and Augmented Dickey–Fuller (ADF) tests.Results
Refugia classifications and DRSI values for all areas were stable over time (1992–2011). The DRSI values were significantly lower than 1; that is, the density of rare species in the predicted refugia areas was higher than in non-refugia areas.Conclusions
We assumed that patches of dense woody vegetation, determined by the upper 25% quantile of the NDVI, could be used to identify potential biodiversity refugia in dry environments. This assumption was validated by the DRSI results; it confirms that the local conditions in refugia support rare species.10.