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Raghavan V.S. Kumanan K. Thirumurugan G. Nachimuthu K. 《Tropical animal health and production》1998,30(5):287-293
Eleven Newcastle disease viruses (NDV), isolated from apparently healthy and ailing Desi chickens were subjected to both conventional and modern characterization techniques. The virulence and strain differentiating experiments placed 10 isolates in the velogenic group and one in the mesogenic group. In MDBK cells, 9 isolates produced characteristic cytopathogenic effects up to 5 and 2 up to 3 passages. Molecular characterization with a 21-mer oligonucleotide probe placed all the isolates in the velogenic/mesogenic group. The results of this study clearly indicated that the isolates obtained are either velogenic or mesogenic but not lentogenic. 相似文献
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Praveena PE Jayakumar R Balachandran C Thirumurugan G Raj GD Manohar BM 《Veterinary research communications》2007,31(6):775-781
Rabies is diagnosed by FAT in the impression smears of brain tissues. In this study, an attempt was made to diagnose rabies
using in situ polymerase chain reaction (ISPCR). A digoxigenin-labelled double-stranded probe specific for a portion of the ‘N’ gene of
rabies virus was used. Positive signals were identified as blue dots in the intraneuronal and neuropil areas. 相似文献
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