Pigmented skin nodule during carcass examination in a fattening pig     

Stephan R  Sydler T  Hlobil Y 《Schweizer Archiv für Tierheilkunde》2002,144(3):138-141

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Ultrasound-guided percutaneous lung biopsy in sheep     

Braun U  Estermann U  Flückiger M  Sydler T  Pospischil A 《The Veterinary record》2000,146(18):525-528

Lung biopsies were taken through the ninth intercostal space on the two sides of the thorax of 10 clinically healthy sheep, using a Tru-Cut needle on one side and the Bard Biopty-System on the other. Nine of the 10 sheep remained clinically healthy, but one coughed transiently and had a mild bloody discharge from the right nostril immediately after the biopsy. The sheep were slaughtered 10 days later and the lungs and pleura were examined macroscopically; there were either no lesions or only small scars visible at the sites of the biopsies. However, well-developed subpleural nodules due to parasites were observed in some of the lungs. There were no adhesions between the costal and pulmonary pleurae. Of the 20 biopsy specimens, 18 were ideal for histological examination, and none of them was histologically normal. Mild interstitial pneumonia was diagnosed in 15 specimens, chronic bronchiolitis in nine specimens, and hyperplasia of smooth muscle or connective tissue in 17.  相似文献   

Short-term response of salal (Gaultheria shallon Pursh) to commercial harvesting for floral greenery     

Wendy Cocksedge  Brian D. Titus 《Agroforestry Systems》2006,68(2):103-111

Salal (Gaultheria shallon Pursh), which is widely used for floral greenery, is an important non-timber forest product (NTFP) from the coastal forests of the Pacific Northwest of North America. However, there are no known studies on the impacts of commercial salal harvesting on subsequent growth. A study was therefore initiated to quantify the growth of salal 1 year after commercial harvesting, and to compare this with growth of unharvested salal. The amount of biomass removed from shrubs through commercial harvesting (131 g m⁻²) was the same as the amount of annual growth in adjacent undisturbed plots (135 g m⁻²). One year later, the amount of regrowth in previously harvested plots (144 g m⁻²) was greater than the amount of new growth in adjacent undisturbed plots (111 g m⁻²). As there was little difference in the weight per current stem, the increased biomass after commercial harvesting was attributed to the observed increase in stem number (60 stems m⁻²) as compared to undisturbed salal (50 stems m⁻²). Our study does not incorporate either repeated annual harvesting or variable harvesting intensities, both of which have been anecdotally reported to affect levels of re-growth and therefore sustainability.  相似文献   

Genetic diversity of whitefly (Bemisia spp.) on crop and uncultivated plants in Uganda: implications for the control of this devastating pest species complex in Africa     

Mugerwa  Habibu  Colvin  John  Alicai  Titus  Omongo  Christopher A.  Kabaalu  Richard  Visendi  Paul  Sseruwagi  Peter  Seal  Susan E. 《Journal of pest science》2021,94(4):1307-1330

Journal of Pest Science - Over the past three decades, highly increased whitefly (Bemisia tabaci) populations have been observed on the staple food crop cassava in eastern Africa and associated...  相似文献   

Adhesive fimbriae produced in vivo by Escherichia coli O139:K12(B):H1 associated with enterotoxaemia in pigs   总被引：8，自引：0，他引：8  

H U Bertschinger  M Bachmann  C Mettler  A Pospischil  E M Schraner  M Stamm  T Sydler  P Wild 《Veterinary microbiology》1990,25(2-3):267-281

Two strains of E. coli O139:K12 (B):H1 were compared in vitro and in the intestinal environment. Both strains colonized the small intestines of experimentally inoculated pigs and exhibited in vivo a similar relationship to the microvillus border as enterotoxigenic E. coli (ETEC). Strain 107/86 grown on blood agar expressed numerous long flexible non-haemagglutinating fimbriae which were antigenically distinct from the known fimbriae of porcine ETEC. It adhered in vitro to porcine enterocyte brush border fragments. Strain 124/76 grown on blood agar was devoid of fimbriae and did not adhere to brush border fragments. However, fimbriae morphologically and antigenically indistinguishable from those of strain 107/86 were detected in the intestinal environment by direct immunofluorescence and by immuno electron microscopy.  相似文献   

Regulation of differentiation to the infective stage of the protozoan parasite Leishmania major by tetrahydrobiopterin     

Cunningham ML  Titus RG  Turco SJ  Beverley SM 《Science (New York, N.Y.)》2001,292(5515):285-287

A critical step in the infectious cycle of Leishmania is the differentiation of parasites within the sand fly vector to the highly infective metacyclic promastigote stage. Here, we establish tetrahydrobiopterin (H4B) levels as an important factor controlling the extent of metacyclogenesis. H4B levels decline substantially during normal development, and genetic or nutritional manipulations showed that low H4B caused elevated metacyclogenesis. Mutants lacking pteridine reductase 1 (PTR1) had low levels of H4B, remained infectious to mice, and induced larger cutaneous lesions (hypervirulence). Thus, the control of pteridine metabolism has relevance to the mechanism of Leishmania differentiation and the limitation of virulence during evolution.  相似文献   

A POSSIBLE PHYSIOLOGICAL INTERPRETATION OF THE LAW OF THE DIMINISHING INCREMENT     

Hendricks WA  Jull MA  Titus HW 《Science (New York, N.Y.)》1931,73(1894):427-429

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Does shelterwood harvesting have less impact on forest floor nutrient availability and microbial properties than clearcutting?     

Robert L. Bradley  Brian D. Titus  Karen Hogg 《Biology and Fertility of Soils》2001,34(3):162-169

Chemical and microbial properties of the forest floor were measured in 4-year-old shelterwood treatment plots in the montane coastal western hemlock biogeoclimatic zone of British Columbia, Canada, and compared to those in adjacent old-growth and 4-year-old clearcut plots. Forest floor pH, exchangeable Mg, total C and total N concentrations were similar in all three treatments. Bray-extractable P was significantly lower in humus from shelterwood plots, exchangeable Ca was significantly lower in humus from clearcut plots, and exchangeable K was significantly higher in humus from old-growth plots. Indices of available C (basal respiration rate, microbial biomass and metabolic quotient) were significantly higher in old-growth plots than in the two harvested treatment plots. The relative increase in maximum microbial metabolic rate due to the addition of organic nutrients was significantly greater in clearcut plots. Principal component analysis (PCA) ordination of humus samples based on C source utilization patterns of microbial communities (i.e., Biolog assay) showed distinctive clustering by treatment, although indices of substrate richness, evenness and diversity were the same in all three treatments. The relative utilization rates of substrates with the highest factor loadings on PCA axes 1 and 2 were dissimilar in each treatment. We conclude that forest floors can develop under shelterwood harvested stands to exhibit chemical and microbial properties atypical of either clearcut or old-growth plots.  相似文献   

Immunohistochemical labeling of Giardia trophozoites spp. in formalin fixed paraffin embedded tissues.     

T Sydler  A Pospischil  B Gottstein  J Eckert 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1991,38(2):135-141

An immunoperoxidase method using rabbit anti human Giardia lamblia serum for the demonstration of giardia in paraffin embedded intestinal animal tissue is described. Specificity was tested against other protozoal parasites.  相似文献   

Experimental alveolar echinococcosis in pigs, lesion development and serological follow up     

Deplazes P  Grimm F  Sydler T  Tanner I  Kapel CM 《Veterinary parasitology》2005,130(3-4):213-222

Liver lesions were found in 6/6 pigs 7 months after oral inoculation with 5000 or 35,000 Echinococcus multilocularis eggs. However, lesion morphology differed considerably among the animals. The largest lesions (3–8 mm in diameter) were found in a single pig and smaller lesions (1.5–3 mm) in three pigs. These lesions were clearly circumscribed and had pronounced central necroses and dystrophic calcifications. In contrast, most of the smallest (usually <1.5 mm in diameter) found in two other pigs, had small compact fibrotic areas and blurred borders with obvious fibrous infiltrations into the interlobular tissues. E. multilocularis specific DNA was detected by PCR in all lesion types, but metacestode viability, as assessed by in vivo intraperitoneal inoculations in jirds, could not be demonstrated. Within 1 month post inoculation, all pigs developed specific IgG antibody responses against a battery of different antigens (metacestode, cyst fluid, and protoscoleces-derived native E. multilocularis and E. granulosus antigens, affinity purified Em2G11 antigen, antigen B, recombinant Em II/3–10 antigen). Two different reaction patterns were recorded. In the two pigs with the small lesions, pronounced reactions against all crude antigens with peaks 3–5 months p.i. and clearly elevated levels until the end of the experiment were noted. In all other pigs, antibody reactions remained low in all cases. In conclusion, we demonstrated two types of E. multilocularis metacestode development in pigs with distinct immunological response patterns.  相似文献