Sporulation of Pyrenopeziza brassicae (light leaf spot) on oilseed rape (Brassica napus) leaves inoculated with ascospores or conidia at different temperatures and wetness durations   总被引：1，自引：0，他引：1  

Z. Karolewski  N. Evans  B. D. L. Fitt  A. D. Todd  A. Baierl 《Plant pathology》2002,51(5):654-665

In controlled environment experiments, sporulation of Pyrenopeziza brassicae was observed on leaves of oilseed rape inoculated with ascospores or conidia at temperatures from 8 to 20°C at all leaf wetness durations from 6 to 72 h, except after 6 h leaf wetness duration at 8°C. The shortest times from inoculation to first observed sporulation ( l ₀), for both ascospore and conidial inoculum, were 11–12 days at 16°C after 48 h wetness duration. For both ascospore and conidial inoculum (48 h wetness duration), the number of conidia produced per cm² leaf area with sporulation was seven to eight times less at 20°C than at 8, 12 or 16°C. Values of Gompertz parameters c (maximum percentage leaf area with sporulation), r (maximum rate of increase in percentage leaf area with sporulation) and l ₃₇ (days from inoculation to 37% of maximum sporulation), estimated by fitting the equation to the observed data, were linearly related to values predicted by inserting temperature and wetness duration treatment values into existing equations. The observed data were fitted better by logistic equations than by Gompertz equations (which overestimated at low temperatures). For both ascospore and conidial inoculum, the latent period derived from the logistic equation (days from inoculation to 50% of maximum sporulation, l ₅₀) of P. brassicae was generally shortest at 16°C, and increased as temperature increased to 20°C or decreased to 8°C. Minimum numbers of spores needed to produce sporulation on leaves were ≈25 ascospores per leaf and ≈700 conidia per leaf, at 16°C after 48 h leaf wetness duration.  相似文献   

Factors affecting the development of disease symptoms in potatoes infected by <Emphasis Type="Italic">Tobacco rattle virus</Emphasis>     

D.J. Robinson  M.F.B. Dale  D. Todd 《European journal of plant pathology / European Foundation for Plant Pathology》2004,110(9):921-928

Infection of potato plants with Tobacco rattle virus by its nematode vector can have different outcomes, including the development of spraing symptoms in progeny tubers. A novel syndrome described here comprises a generalized mottle in leaves on all stems, together with virtually total failure to produce daughter tubers. The outcome of infection depends partly on the potato genotype, but field trials with 15 varieties showed that the incidence and severity of spraing symptoms at three sites differed. There was no evidence that this was due to differences in virulence among the virus isolates at the sites, but it was probably the result of environmental differences that influenced the numbers and activity of the vector nematodes. At the most severely affected site, spraing symptoms were found in all varieties tested, except Record, including several that were not affected at the other two sites. Taking both severity and incidence of symptoms into account, the ranking of varieties was similar at each site. The incidence of spraing symptoms was greater in larger than in smaller tubers, and increased with later harvest dates, but did not increase when early harvested tubers were stored. Tobacco rattle viruswas detected in the roots of many of the weeds growing at one of the sites and in the roots of a few plants of the subsequent barley crop.  相似文献   

Randomly primed,strand-switching,MinION-based sequencing for the detection and characterization of cultured RNA viruses     

Kelsey T. Young  Kevin K. Lahmers  Holly S. Sellers  David E. Stallknecht  Rebecca L. Poulson  Jerry T. Saliki  Stephen Mark Tompkins  Ian Padykula  Chris Siepker  Elizabeth W. Howerth  Michelle Todd  James B. Stanton 《Journal of veterinary diagnostic investigation》2021,33(2):202

RNA viruses rapidly mutate, which can result in increased virulence, increased escape from vaccine protection, and false-negative detection results. Targeted detection methods have a limited ability to detect unknown viruses and often provide insufficient data to detect coinfections or identify antigenic variants. Random, deep sequencing is a method that can more fully detect and characterize RNA viruses and is often coupled with molecular techniques or culture methods for viral enrichment. We tested viral culture coupled with third-generation sequencing for the ability to detect and characterize RNA viruses. Cultures of bovine viral diarrhea virus, canine distemper virus (CDV), epizootic hemorrhagic disease virus, infectious bronchitis virus, 2 influenza A viruses, and porcine respiratory and reproductive syndrome virus were sequenced on the MinION platform using a random, reverse primer in a strand-switching reaction, coupled with PCR-based barcoding. Reads were taxonomically classified and used for reference-based sequence building using a stock personal computer. This method accurately detected and identified complete coding sequence genomes with a minimum of 20× coverage depth for all 7 viruses, including a sample containing 2 viruses. Each lineage-typing region had at least 26× coverage depth for all viruses. Furthermore, analyzing the CDV sample through a pipeline devoid of CDV reference sequences modeled the ability of this protocol to detect unknown viruses. Our results show the ability of this technique to detect and characterize dsRNA, negative- and positive-sense ssRNA, and nonsegmented and segmented RNA viruses.  相似文献   

Nutritional analysis and microbiological evaluation of commercially available enteral diets for cats          下载免费PDF全文

Lori R. Prantil MPS  DVM  Jessica E. Markovich DVM  DACVIM  Cailin R. Heinze VMD  MS  DACVN  Deborah E. Linder DVM  DACVN  Todd R. Tams DVM  DACVIM  Lisa M. Freeman DVM  PhD  DACVN 《Journal of Veterinary Emergency and Critical Care》2016,26(2):254-261

  相似文献   

Effect of Regional Intravenous Limb Perfusate Volume on Synovial Fluid Concentration of Amikacin and Local Venous Blood Pressure in the Horse          下载免费PDF全文

Darla K. Moser  Mike J. Schoonover  Todd C. Holbrook  Mark E. Payton 《Veterinary surgery : VS》2016,45(7):851-858

  相似文献   

Imidacloprid-resistant Aphis gossypii populations are more common in cotton-dominated landscapes     

John W. Mahas  Todd D. Steury  Anders S. Huseth  Stephanie R Rogers  Alana L. Jacobson 《Pest management science》2023,79(3):1040-1047

  相似文献   

Subsurface drip irrigation for corn production: a review of 10 years of research in Kansas   总被引：5，自引：0，他引：5  

Freddie R. Lamm  Todd P. Trooien 《Irrigation Science》2003,22(3-4):195-200

Kansas State University initiated studies in 1989 to develop the methodology for successful application of subsurface drip irrigation (SDI) for corn production on the deep silt loam soils of the Central Great Plains, USA. Irrigation water use for corn can be reduced by 35–55% when using SDI compared with more traditional forms of irrigation in the region. Irrigation frequency has not been a critical issue when SDI is used for corn production on the deep silt loam soils of the region. A dripline spacing of 1.5 m has been found to be most economical for corn grown in 0.76 m spaced rows. Nitrogen fertigation was a very effective management tool with SDI, helping to maximize corn grain yield, while obtaining high efficiencies of nitrogen and water use. The research SDI systems have been utilized since 1989 without replacement or major degradation. SDI systems lasting 10–20 years are cost competitive for corn production with the more traditional forms of irrigation in the Great Plains for certain field sizes.Communicated by P. Thorburn  相似文献   

Biological Degradation of Common Pharmaceuticals and Personal Care Products in Soils with High Water Content     

Deborah L. Carr  Audra N. Morse  John C. Zak  Todd A. Anderson 《Water, air, and soil pollution》2011,217(1-4):127-134

Biological degradation rates of six pharmaceuticals and personal care products were examined in soil from a land application site and in adjacent soil with no prior history of effluent exposure. Microbial degradation rates were compared over 2 weeks under standing water or saturated conditions and draining conditions after having been saturated for 3 days. Biological degradation of 17??-estradiol exhibited rapid rates of biological degradation under both saturated and draining conditions. Half-lives for 17??-estradiol ranged from 1.5 to 4 days; 66?C97% was lost from the soils. Estriol showed a pattern of biological degradation in both saturated and draining conditions though the half-lives were longer (8.7?C25.9 days) than those observed for 17??-estradiol. Twenty-eight percent to 73% of estriol was lost over the 14 days treatment period. Estrone and 17??-ethinylestradiol exhibited slower rates of biological transformation under saturated and draining conditions. Half-lives for estrone ranged between 27.5 and 56.8 days with loss of at most 21%. 17??-ethinylestradiol exhibited half-lives of 22.6?C207 days. Half-life data for ibuprofen ranged from 30.4 to 1,706.4 days in this experiment. Losses of up to 17% were observed in draining soils. Triclosan loss was at most 10%, and half-lives were 70.9?C398.8 days. In all cases, soils that were draining from saturated conditions exhibited faster degradation rates than soils that remained saturated. Prior exposure of the soil to effluent did not always result in higher biological degradation rates.  相似文献   

Can giant clam (Tridacna squamosa) populations be restored on Singapore's heavily impacted coral reefs?     

James R. Guest  Peter A. Todd  Eugene Goh  Balasubramaniam S. Sivalonganathan  Konda P. Reddy 《水产资源保护:海洋与淡水生态系统》2008,18(5):570-579

• 1. Giant clams have been a sustainable resource for millennia, but unregulated harvesting has led to local extinctions within the Indo‐Pacific region. Giant clam mariculture can produce large numbers of juveniles for restocking wild populations where natural recruitment is low or absent.
• 2. Singapore is surrounded by more than 60 small islands, many with fringing reefs. These reefs, however, experience increased turbidity and sedimentation resulting from massive coastal development projects and regular dredging of shipping lanes.
• 3. Seven reefs off Singapore's southern islands were surveyed (9670 m²) for giant clams. Also, an experiment was conducted to determine the growth of Tridacna squamosa reared in aquaria under three light treatments: ～50% ambient photosynthetically active radiation (PAR); ～25% ambient PAR; and ～12% ambient PAR. Finally, 144 clams (T. squamosa) were transplanted to four reefs around Singapore to study survival and growth in a heavily impacted environment.
• 4. A total of 23 adult clams from three species were found during the survey, representing a mean density of 0.24 per 100 m². Most clams were found at Raffles Lighthouse, Singapore's ‘best’ reef. No juvenile clams were encountered. In the aquarium experiment, clam growth was significantly different among the three light treatments, with growth greatest in the ～50% ambient PAR treatment. Of the 144 transplanted clams, 116 (80.6%) were recovered after 7 months. All specimens had increased in size, with growth rates among reefs ranging from 3.3 mm month^?1 (SD=1.3 mm) to 4.8 mm month^?1 (SD=1.6 mm).
• 5. Results suggest that, despite high levels of sedimentation and turbidity on Singapore's reefs, giant clams can survive and grow well. Restocking efforts using maricultured clams may be effective in enhancing the dwindling local populations. It is not clear, however, whether a self‐sustaining community can be established as high sedimentation may hinder larval settlement and survival.

Copyright © 2007 John Wiley & Sons, Ltd.  相似文献   

Thermal performance curves based on field movements reveal context-dependence of thermal traits in a desert ectotherm     

Nowakowski  A. Justin  Peaden  J. Mark  Tuberville  Tracey D.  Buhlmann  Kurt A.  Todd  Brian D. 《Landscape Ecology》2020,35(4):893-906

Landscape Ecology - Thermal traits likely mediate organismal responses to changing thermal environments. As temperatures increase, predicting species responses will depend on understanding how...  相似文献