Starvation tolerance of newly metamorphosed abalone Haliotis discus hannai   总被引：1，自引：0，他引：1  

Hideki Takami  Tomohiko Kawamura  Yoh Yamashita 《Fisheries Science》2000,66(6):1180-1182

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Characterization of Campylobacter lanienae from pig feces     

Sasaki Y  Fujisawa T  Ogikubo K  Ohzono T  Ishihara K  Takahashi T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(1):129-131

To isolate Campylobacter spp., the feces of healthy cattle, pigs, and broilers were examined between June 1999 and January 2000. Campylobacter lanienae strains were isolated from the feces of healthy pigs, but not from the feces of cattle or broilers. In six C. lanienae isolates, there was only 21-38% DNA-DNA homology to Campylobacter hyointestinalis subsp. lawsonii strain NCTC 12901. Thus, the primary host of C. lanienae is likely to be the pig and C. lanienae appears to be a species distinct from C. hyointestinalis subsp. lawsonii. In addition, an intervening sequence of 226 bp in the 16S rRNA gene was found in four isolates.  相似文献   

Exophthalmos Caused by Chronic Sinusitis in a Horse: A Case of Exophthalmos Caused by Sinusitis     

Teppei Kanda DVM  Yoshiaki Hikasa DVM  PhD  Misaki Naota  Takehito Morita DVM  PhD  Akinori Shimada DVM  PhD  Tomohiko Amaya DVM 《Journal of Equine Veterinary Science》2007,27(12):539-541

A 12-year-old Thoroughbred gelding exhibited a unilateral exophthalmos on the left side. There had been no other clinical symptoms, such as rhinorrhea, before the first consultation. Clinical, hematologic, plasma biochemical, cytologic, and computed tomography (CT) examinations and complete necropsy were performed. These examinations did not reveal neoplastic lesions. CT images suggested that maxillary and frontal sinuses may have been filled with fluid. Cytologic and pathologic examinations revealed that the maxillary and orbital bones were pushed up with a large amount of the inflammatory fluid effused by chronic sinusitis. The CT and cytologic examinations were of great use for differential diagnosis in this case. This was an unusual case of exophthalmos induced by deformation of the orbital bones resulting from chronic sinusitis.  相似文献   

Lethal effects of nonylphenol on fertilized eggs and larvae of marbled sole Pleuronectes yokohamae     

Gen Kume  Toshihiro Horiguchi  Akihiro Goto  Tomohiko Isobe  Shigeko Serizawa  Hiroaki Shiraishi  Masatoshi Morita 《Fisheries Science》2006,72(1):217-219

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Vertical movements of bigeye tuna (Thunnus obesus) associated with islands, buoys, and seamounts near the main Hawaiian Islands from archival tagging data   总被引：5，自引：0，他引：5  

Michael K. Musyl  Richard W. Brill  Christofer H. Boggs  Daniel S. Curran  Thomas K. Kazama  Michael P. Seki 《Fisheries Oceanography》2003,12(3):152-169

To learn more about the movement patterns of bigeye tuna (Thunnus obesus), we deployed archival tags on 87 fish ranging in fork length from 50 to 154 cm. Thirteen fish were recaptured, from which 11 archival tags were returned, representing in aggregate 943 days‐at‐liberty. We successfully retrieved data from 10 tags, representing 474 days in aggregate. The largest fish recaptured was 44.5 kg [131 cm fork length (FL)] and the smallest 2.8 kg (52 cm). The deepest descent recorded was 817 m, the coldest temperature visited 4.7°C, and minimum oxygen level reached ～1 mL L^?1. Fish spent little time at depths where water temperatures were below 7°C and oxygen levels less than ～2 mL L^?1. Five fish were recaptured near the offshore weather buoy where they were tagged. Based on vertical movement patterns, it appeared that all stayed immediately associated with the buoy for up to 34 days. During this time they remained primarily in the uniform temperature surface layer (i.e. above 100 m). In contrast, fish not associated with a floating object showed the W‐shaped vertical movement patterns during the day characteristic of bigeye tuna (i.e. descending to ～300–500 m and then returning regularly to the surface layer). Four fish were tagged and subsequently recaptured near Cross Seamount up to 76 days later. These fish exhibited vertical movement patterns similar to, but less regular than, those of fish not associated with any structure. Bigeye tuna appear to follow the diel vertical movements of the deep sound scattering layer (SSL) organisms and thus to exploit them effectively as a prey resource. Average night‐time depth was correlated with lunar illumination, a behaviour which mimics movements of the SSL.  相似文献   

Dietary value of benthic diatoms for post-larval abalone <Emphasis Type="Italic">Haliotis diversicolor</Emphasis> associated with feeding transitions     

Toshihiro Onitsuka  Tomohiko Kawamura  Satoshi Ohashi  Toyomitsu Horii  Yoshiro Watanabe 《Fisheries Science》2007,73(2):295-302

ABSTRACT:   The feeding behavior and growth of post-larval Haliotis diversicolor with initial shell lengths (SL) of approximately 500 μm (Exp. 1-1 and 1-2), 800 μm (Exp. 2), and 1200 μm (Exp. 3) were studied in a laboratory setting while they fed on four species of benthic diatom Achnanthes longipes , Cocconeis sublittoralis , Cylindrotheca closterium , and Navicula ramosissima . Exp. 1-1 and 1-2 revealed no marked differences in post-larval growth rates (mean 24–39 μm SL/day) among the diatom species. However, marked differences in growth rates among the species were revealed in Exp. 2 and 3. Three species, A. longipes , Co. sublittoralis, and Cy. closterium , produced faster growth (Exp. 2 mean 29–51 μm/day, Exp. 3 mean 36–44 μm/day) than N. ramosissima (Exp. 2 mean 18 μm/day, Exp. 3 mean 23 μm/day). Post-larvae fed N. ramosissima had lower digestion efficiency (42.8%) than those fed other diatom species (90.7–100%). Diatom extracellular substances appeared to be principally used from post-settlement to 800 μm SL, and diatom cell contents were required to produce rapid growth of larger post-larvae (>800 μm SL). It is likely that the availability of each diatom for post-larvae was affected by diatom morphology, attachment strength, frustule strength, and post-larval size.  相似文献   

Two male sterility-inducing cytoplasms of beet (Beta vulgaris) are genetically distinct but have closely related mitochondrial genomes: implication of a substoichiometric mitochondrial DNA molecule in their evolution     

Yasuyuki Onodera  Takumi Arakawa  Rika Yui-Kurino  Masayuki P. Yamamoto  Kazuyoshi Kitazaki  Shigehiko Ebe  Muneyuki Matsunaga  Kazunori Taguchi  Yosuke Kuroda  Shiko Yamashita  Tomoyuki Sakai  Toshiro Kinoshita  Tetsuo Mikami  Tomohiko Kubo 《Euphytica》2015,206(2):365-379

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Effects of heating on the interaction of lipid and zein in a dry powder system     

Mizutani Y  Matsumura Y  Murakami H  Mori T 《Journal of agricultural and food chemistry》2004,52(11):3570-3576

The effects of heat treatment on the interaction of lipid and zein in a dry powder system were investigated. Linolenic acid ethyl ester (LAE) was mixed with the zein powder. The glass transition temperature (T(g)) for the dry powder zein was shown to be approximately 107 degrees C by differential scanning calorimetry. The thermogram of the zein-LAE mixed powder showed an exothermic transition near the T(g) of zein. Fourier transform infrared (FT-IR) spectroscopy was used for detecting the structural changes in zein by heat treatment, that is, elevating the temperature from 25 to 160 degrees C. The heat treatment of the powdery zein with and without LAE caused increases in the alpha-helix, beta-turn, and beta-sheet, concomitant with decreases in the intermolecular hydrogen-bonded beta-sheet and random coil. Such changes in the secondary structure were more drastic for the powder with LAE. The heating of the zein-LAE mixed powder also caused decreases in the peaks originating from LAE in the FT-IR spectra. These results suggest that the heat treatment induced the interaction of the zein and LAE in the powdery system. The influence of heating on the antioxidative activity of dry powder zein was studied by measurements of the peroxide value. When zein-LAE mixed powder was heated before storage, the oxidation of LAE was inhibited for 7 days, whereas LAE was oxidized within 1 day in the absence of heat treatment.  相似文献   

Effects of protein and peptide addition on lipid oxidation in powder model system     

Park EY  Murakami H  Mori T  Matsumura Y 《Journal of agricultural and food chemistry》2005,53(1):137-144

The effect of protein and peptide addition on the oxidation of eicosapentaenoic acid ethyl ester (EPE) encapsulated by maltodextrin (MD) was investigated. The encapsulated lipid (powder lipid) was prepared in two steps, i.e., mixing of EPE with MD solutions (+/- protein and peptides) to produce emulsions and freeze-drying of the resultant emulsions. EPE oxidation in MD powder progressed more rapidly in the humid state [relative humidity (RH) = 70%] than in the dry state (RH = 10%). The addition of soy protein, soy peptide, and gelatin peptides improved the oxidation stability of EPE encapsulated by MD, and the inhibition of lipid oxidation by the protein and the peptides was more dramatic in the humid state. Especially, the oxidation of EPE was almost perfectly suppressed when the lipid was encapsulated with MD + soy peptide during storage in the humid state for 7 days. Several physical properties such as the lipid particle size of the emulsions, the fraction of nonencapsulated lipids, scanning electron microscopy images of powder lipids, and the mobility of the MD matrix were investigated to find the modification of encapsulation behavior by the addition of the protein and peptides, but no significant change was observed. On the other hand, the protein and peptides exhibited a strong radical scavenging activity in the powder systems as well as in the solution systems. These results suggest that a chemical mechanism such as radical scavenging ability plays an important role in the suppression of EPE oxidation in MD powder by soy proteins, soy peptides, and gelatin peptides.  相似文献   

Effects of Ca2+ and sulfhydryl reductant on the polymerization of soybean glycinin catalyzed by mammalian and microbial transglutaminases     

Zhang G  Matsumura Y  Matsumoto S  Hayashi Y  Mori T 《Journal of agricultural and food chemistry》2003,51(1):236-243

Two types of transglutaminases (TGases), Ca(2+)-dependent TGase derived from guinea pig liver (GTGase) and Ca(2+)-independent TGase derived from a variant of Streptoverticillium mobaraense (MTGase), were used to study the cross-linking of soybean 11S globulin (glycinin). The effects of sulfhydryl reductant (dithiothreitol, DTT) and Ca(2+) on the conformation and TGase-catalyzed polymerization of glycinin were investigated. The conformational change of glycinin was probed by spectral methods. The degree of cross-linking and the polymer (aggregate) formation were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and dynamic light scattering, respectively. Addition of DTT stimulated the TGase-catalyzed cross-linking reactions without destroying the secondary and tertiary structure of glycinin but did not influence the polymer or aggregate formation. It was found that Ca(2+) caused the formation of larger size polymers at lower concentrations, while it suppressed the polymerization at higher concentrations. In addition, the cross-linking behaviors of glycinin were shown to be different between MTGase- and GTGase-catalyzed systems.  相似文献