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Haruka SASAKI Kiyoko WATANABE Toshizo TOYAMA Yasunori KOYATA Nobushiro HAMADA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(3):265-271
Porphyromonas gulae is considered to be associated with canine
periodontitis. We have previously reported that the P. gulae American
Type Culture Collection (ATCC) 51700 comprised 41-kDa fimbriae. The purpose of the present
study was to demonstrate the roles of 41-kDa fimbrial protein in periodontal disease. In
this study, we examined the involvement of the 41-kDa fimbrial protein in osteoclast
differentiation and cytokine production in murine macrophages. Furthermore, alveolar bone
resorption induced by P. gulae infection in rats was evaluated. To
estimate osteoclast differentiation, bone marrow cells and MC3T3-G2/PA6 cells were
cultured with or without the 41-kDa fimbrial protein for 7 days. BALB/c mouse peritoneal
macrophages were stimulated with the 41-kDa fimbrial protein, and the levels of
interleukin (IL)-1β and tumor necrosis factor (TNF)-α production were determined by
enzyme-linked immunosorbent assay. Osteoclast differentiation was significantly enhanced
by treatment with the 41-kDa fimbrial protein in a dose-dependent manner. The total area
of pits formed on the dentine slices with osteoclasts incubated with the 41-kDa fimbrial
protein was significantly greater than that of the control. The purified 41-kDa fimbrial
protein induced IL-1β and TNF-α production in BALB/c mouse peritoneal macrophages after 6
hr of incubation in a dose-dependent manner. The bone loss level in rats infected with
P. gulae was significantly higher than that of the sham-infected rats.
These results suggest that P. gulae 41-kDa fimbriae play important roles
in the pathogenesis of periodontal disease. 相似文献
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