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鸡传染性鼻炎流行病学调查(Ⅱ)   总被引:5,自引:0,他引:5  
利用鸡传染性鼻炎PCR试剂盒和阻断ELISA试剂盒,对29份菌株、136份临床样品和1019份血清进行了检验和分析,结果PCR阳性率为36.4%,A型阳性率为10.3%,C型阳性率为6.4%。  相似文献   
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巴氏杆菌病又叫出血性败血症,简称出败,主要是由多杀性巴氏杆菌所引起的各种畜禽和野生动物感染的一种传染病。临床特征急性病例主要表现为败血症和炎性出血,慢性特征表现为皮下组织、关节、各脏器的局灶性化脓性炎症。该病常在畜禽中发生,而在狐狸等经济动物方面报道相对较少。2006年秋,吉林左家某狐场的狐狸突然发病并陆续死亡,经流行病学调查、病理剖检和实验室检验,确诊为多杀性巴氏杆菌感染所致。  相似文献   
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To improve the Boer goat semen quality during cryopreservation process, three experiments were carried out to investigate the effect of (i) different concentration of ascorbic acid supplementation (ii) rate of cooling with chilled semen characteristics and (iii) method of freezing on post‐thaw Boer goat sperm using Tris‐based extender. Ascorbic acid at 8.5 mg/ml improved the sperm parameters (motility, integrity of membrane and acrosome, morphology and viability), compared to control in cooled samples (p < 0.05). With regard to other concentrations and post‐thawed parameters, ascorbic acid at 2.5–8.5 mg/ml led to higher percentages of sperm motility and integrities of membrane and acrosome when compared to control (p < 0.05). Slow cooling rises to higher percentages of sperm motility, acrosome integrity and viability, in comparison with fast cooling, in terms of cooled and frozen samples (p < 0.05). Programmable freezing method produced the higher percentages of sperm motility, integrities of membrane and acrosome and viability when compared to the freezing method of polystyrene box during goat sperm freezing (p < 0.05). In conclusion, chilled and post‐thawed sperm quality of Boer goat was improved when a Tris‐based extender supplemented with ascorbic acid was used at stages of different cooling rates and freezing methods.  相似文献   
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Trypanosoma vivax and Trypanosoma evansi are livestock parasites of economic importance in Africa, Asia and South America. In the Pantanal, Brazil, they cause economic losses in both cattle and equines. Little is known of their maintenance and spread in nature, particularly in terms of reservoirs and means of mechanical transmission. Here we report for the first time the use of PCR for the detection of T. vivax and T. evansi in bovines, buffaloes and sheep. Whereas parasitological diagnosis detected only two T. vivax infections, one in buffalo and another in a cow, PCR detected infections in 34.8% buffaloes, 44.7% bovines and 37.3% sheep. Trypanozoon primers detected 41.8% infections in buffaloes and 8.1% in cattle. PCR revealed 6.9% mixed infections in buffaloes and 5.3% in cattle. The potential role of cattle and buffaloes as hosts and reservoirs of T. vivax is discussed, as well as the implications of possible extravascular foci in the maintenance of livestock trypanosomosis.  相似文献   
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