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1.
Murat Kutlu Onder Ergonul Selda Sayin-Kutlu Tumer Guven Cemal Ustun Sema Alp-Cavus Serife Barcın Ozturk Ozlem Acicbe Serife Akalin Recep Tekin Suda Tekin-Koruk Yusuf Ziya Demiroglu Ramazan Keskiner Ibak Gönen Sevil Sapmaz-Karabag Vuslat Bosnak Esra Kazak 《Preventive veterinary medicine》2014
Veterinarians and veterinary technicians are at risk for occupational brucellosis. We described the risk factors of occupational brucellosis among veterinary personnel in Turkey. A multicenter retrospective survey was performed among veterinary personnel who were actively working in the field. Of 712 veterinary personnel, 84 (11.8%) had occupational brucellosis. The median number of years since graduation was 7 (interquartile ranges [IQR], 4–11) years in the occupational brucellosis group, whereas this number was 9 (IQR, 4–16) years in the non-brucellosis group (p < 0.001). In multivariable analysis, working in the private sector (odds ratio [OR], 2.8; 95% confidence interval [95% CI], 1.55–5.28, p = 0.001), being male (OR, 4.5; 95% CI, 1.05–18.84, p = 0.041), number of performed deliveries (OR, 1.01; 95% CI, 1.002–1.02, p = 0.014), and injury during Brucella vaccine administration (OR, 5.4; 95% CI, 3.16–9.3, p < 0.001) were found to be risk factors for occupational brucellosis. We suggest that all veterinary personnel should be trained on brucellosis and the importance of using personal protective equipment in order to avoid this infection. 相似文献
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S Yildiz D Blache F Celebi I Kaya M Saatci M Cenesiz B Guven 《Reproduction in domestic animals》2003,38(3):182-186
The aim of the current study was to evaluate the effects of high carbohydrate or fat diets, fed for 15 days at the end of breeding season, on leptin, GH and LH secretions in prepubertal fat‐tailed Tuj lambs. For that purpose, 9‐month‐old ram‐lambs were divided into three groups as control group (fed with basal ration, n = 4), high carbohydrate (HC) group, basal ration plus barley, n = 4), or high fat (HF) group (basal ration plus by‐pass fat, n = 4). For the measurement of leptin and GH, blood plasma samples were collected on days 1, 4, 9 and 14 of the experiment. For the measurement of LH pulse frequency, serial blood samples were collected every 15 min for 6 h on day 14. Lambs were weighed and body condition scored (BCS) on days 1 and 15. Body weight and BCS increased towards the end of the study (p < 0.05). The BCS was higher in high energy groups at the end of the experiment (p < 0.05). Diet affected plasma leptin concentrations (p = 0.002) but time did not. The GH concentrations were not affected by diet or time. The LH pulse frequency appeared to be higher in HC and HF groups but there were no statistical difference between the groups. There was a significant positive relationship between overall BCS and corresponding leptin concentrations (R2 = 0.263; p = 0.010) and between LH pulse frequency and leptin concentrations (R2 = 0.594; p = 0.003). In conclusion, the present study suggests that rather than type of energy, amount of energy intake and body energy reserves are much important regulators of plasma leptin concentrations and LH pulse frequency in fat‐tailed Tuj lambs. 相似文献
3.
ABSTRACT Ribosome-inactivating proteins (RIPs) are N-glycosidases that remove specific purine residues from the sarcin/ricin (S/R) loop of the large rRNA and arrest protein synthesis at the translocation step. In addition to their enzymatic activity, RIPs have been reputed to be potent antiviral agents against many plant, animal, and human viruses. We recently showed that pokeweed antiviral protein (PAP), an RIP from pokeweed, inhibits translation in cell extracts by binding to the cap structure of eukaryotic mRNA and viral RNAs and depurinating these RNAs at multiple sites downstream of the cap structure. In this study, we examined the activity of three different RIPs against capped and uncapped viral RNAs. PAP, Mirabilis expansa RIP (ME1), and the Saponaria officinalis RIP (saporin) depurinated the capped Tobacco mosaic virus and Brome mosaic virus RNAs, but did not depurinate the uncapped luciferase RNA, indicating that other type I RIPs besides PAP can distinguish between capped and uncapped RNAs. We did not detect depurination of Alfalfa mosaic virus (AMV) RNAs at multiple sites by PAP or ME1. Because AMV RNAs are capped, these results indicate that recognition of the cap structure alone is not sufficient for depurination of the RNA at multiple sites throughout its sequence. Furthermore, PAP did not cause detectable depurination of uncapped RNAs from Tomato bushy stunt virus (TBSV), Satellite panicum mosaic virus (SPMV), and uncapped RNA containing poliovirus internal ribosome entry site (IRES). However, in vitro translation experiments showed that PAP inhibited translation of AMV, TBSV, SPMV RNAs, and poliovirus IRES dependent translation. These results demonstrate that PAP does not depurinate every capped RNA and that PAP can inhibit translation of uncapped viral RNAs in vitro without causing detectable depurination at multiple sites. Thus, the cap structure is not the only determinant for inhibition of translation by PAP. 相似文献
4.
O'neill TJ Kerrick AD Ait-Ouamer F Tumer OT Zych AD White RS 《Science (New York, N.Y.)》1989,244(4903):451-454
Gamma rays at energies of 0.3 to 8 megaelectron volts (MeV) were detected on 15 April 1988 from four nuclear-powered satellites including Cosmos 1900 and Cosmos 1932 as they flew over a double Compton gamma-ray telescope. The observations occurred as the telescope, flown from a balloon at an altitude of 35 kilometers from Alice Springs, Australia, searched for celestial gamma-ray sources. The four transient signals were detected in 30 hours of data. Their time profiles show maxima with durations of (21 +/- 1) and (27 +/- 1) seconds (half-width at half maximum) for the lower two satellites and (85 +/- 5) and (113 +/- 7) seconds for the remaining two. Their durations place the origin of the two shorter signals at orbital radii of 260(+40)(-60) and 260 +/- 60 km above the earth and the two longer at 800(+100)(-300) and 800(+250)(-300) kilometers. Their luminosities for energies >0.3 MeV are then (6.1 +/- 1.5) x 10(15), (3.9 +/- 1.0) x 10(15), (1.10 +/- 0.28) x 10(16), and (1.30 +/- 0.32) x 10(16) photons per second. The imaging of the strongest signal indicates a southeastern direction passing nearly overhead. The energy spectrum can be fit to an exponential with index 2.4 +/- 1.4. These transient events add to the already large backgrounds for celestial gamma ray sources. 相似文献
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Shah DM Horsch RB Klee HJ Kishore GM Winter JA Tumer NE Hironaka CM Sanders PR Gasser CS Aykent S Siegel NR Rogers SG Fraley RT 《Science (New York, N.Y.)》1986,233(4762):478-481
The herbicide glyphosate is a potent inhibitor of the enzyme 5-enolpyruvylshikimate- 3-phosphate (EPSP) synthase in higher plants. A complementary DNA (cDNA) clone encoding EPSP synthase was isolated from a complementary DNA library of a glyphosate-tolerant Petunia hybrida cell line (MP4-G) that overproduces the enzyme. This cell line was shown to overproduce EPSP synthase messenger RNA as a result of a 20-fold amplification of the gene. A chimeric EPSP synthase gene was constructed with the use of the cauliflower mosaic virus 35S promoter to attain high level expression of EPSP synthase and introduced into petunia cells. Transformed petunia cells as well as regenerated transgenic plants were tolerant to glyphosate. 相似文献
7.
Guven Hakan Everhart Sydney E. De Miccolis Angelini Rita Milvia Ozkilinc Hilal 《植物病害和植物保护杂志》2021,128(6):1459-1465
Journal of Plant Diseases and Protection - Monilinia fructicola, causal agent of brown rot of stone fruits, is an economically important problem worldwide. Six of the sequence tagged microsatellite... 相似文献
8.
Esra Ucar Yasar Ozyigit Ahmet Demirbas Dilek Yasin Guven Kenan Turgut 《Communications in Soil Science and Plant Analysis》2017,48(10):1231-1239
With this study, the effect of different nitrogen (N) doses applied to sweet herb (0, 50, 100, 150 and 200 N kg ha?1) on the macro/micro nutrient element content, dry matter ratio and on the chlorophyll amount in the plant leaves is examined. The research was carried out in the research and trial field of Faculty of Agriculture Agronomy department, Akdeniz University, during the 2012–2013 growing season under field conditions for 2 years with four replications according to the randomized block trial design. Chlorophyll amounts were determined and the average was obtained with three readings from each leaf by a chlorophyll meter when the plants were in the beginning of flowering. Post-harvest macro and micro nutrient elements of the sweet herb were also investigated. According to the obtained data, while the highest chlorophyll and N amounts in the leaf for both the first and second years were determined as 46.97 SPAD (Minolta Corp, NJ, USA) and 1.34 N% 44.9 SPAD, 1.42 N%, respectively, under 200 kg ha?1 N application, the lowest chlorophyll and N amounts were determined in the control group. In addition, N application in different doses for both years increased the phosphorus (P) and potassium (K) contents and increased the microelement contents, especially in the second year. At the same time, a correlation between the chlorophyll content in the plant leaves and the N amount was determined and a linear increase was observed in the chlorophyll amount with the increased green part. 相似文献
9.
F. Koc K. Uney M. Atamanalp I. Tumer G. Kaban 《Aquaculture (Amsterdam, Netherlands)》2009,295(1-2):142-144
In this study, the pharmacokinetic profile of enrofloxacin (EF) and its major metabolite, ciprofloxacin (CF), were investigated in brown trout (Salmo trutta fario) (n = 150) after intravenous (i.v.) and oral (p.o.) administrations of a single dose of 10 mg kg− 1 body weight (b.w.) at 10 °C. The plasma concentrations of the drugs were determined by high-performance liquid chromatography (HPLC-UV) from 0.08 to 120 h. Pharmacokinetic parameters were described by the two-compartment open model for intravenous and oral administrations, respectively. After intravenous administration, the elimination half-life (t1/2β), apparent volume of distribution at steady-state (Vss) and total body clearance (Cltot) of enrofloxacin were 19.14 ± 1.51 h, 3.40 ± 0.18 L kg− 1 and 0.14 ± 0.01 L kg h− 1, respectively. After oral administration, the maximum plasma concentration (Cmax), time of maximum concentration (tmax) and bioavailability (F%) were 2.30 ± 0.08 µg mL− 1, 8 h and 78 ± 4%, respectively. Ciprofloxacin was not detected in the present study. The elimination half-life for enrofloxacin following oral administration was longer than values calculated for other animals. After oral administration, the mean plasma concentration was well above the minimum inhibitory concentrations (MICs)—that is, > 0.5 µg mL− 1 at 36 h—for most gram-negative fish pathogens. It is possible and practical to obtain therapeutic blood concentrations of enrofloxacin in brown trout (S. trutta fario) using oral administration of 10 mg kg− 1 body weight; therefore, it may be effective in the therapy for brown trout diseases. 相似文献
10.
Expression of a bacterial ice nucleation gene in a yeast Saccharomyces cerevisiae and its possible application in food freezing processes 总被引：2，自引：0，他引：2
Hwang WZ Coetzer C Tumer NE Lee TC 《Journal of agricultural and food chemistry》2001,49(10):4662-4666
A 3.6 kb ice nucleation gene (ina) isolated from Erwinia herbicola was placed under control of the galactose-inducible promoter (GAL1) and introduced into Saccharomyces cerevisiae. Yeast transformants showed increased ice nucleation activity over untransformed controls. The freezing temperature of a small volume of water droplets containing yeast cells was increased from approximately -13 degrees C in the untransformed controls to -6 degrees C in ina-expressing (Ina(+)) transformants. Lower temperature growth of Ina(+) yeast at temperatures below 25 degrees C was required for the expression of ice nucleation activity. Shift of temperature to 5-20 degrees C could induce the ice nucleation activity of Ina(+) yeast when grown at 25 degrees C, and maximum ice nucleation activity was achieved after induction at 5 degrees C for approximately 12 h. The effects of Ina(+) yeast on freezing and texturization of several food materials was also demonstrated. 相似文献