RADIOGRAPHIC DIAGNOSIS: PERICARDIOPERITONEAL DIAPHRAGMATIC HERNIA AND CHOLELITHIASIS IN A DOG     

Diana S.  Rosenstein  DVM  Ullrich  Reif  DVM  Russ L.  Stickle  DVM  Gary  Watson  DVM  William  Schall  DVM  Pierre  Amsellem  DVM 《Veterinary radiology & ultrasound》2001,42(4):308-310

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The Role of Ethylene Production in Virulence of Pseudomonas syringae pvs. glycinea and phaseolicola     

Weingart H  Ullrich H  Geider K  Völksch B 《Phytopathology》2001,91(5):511-518

ABSTRACT The importance of ethylene production for virulence of Pseudomonas syringae pvs. glycinea and phaseolicola was assayed by comparing bacterial multiplication and symptom development in bean and soybean plants inoculated with ethylene-negative (efe) mutants and wild-type strains. The efe mutants of Pseudomonas syringae pv. glycinea were significantly reduced in their ability to grow in planta. However, the degree of reduction was strain-dependent. Population sizes of efe mutant 16/83-E1 that did not produce the phototoxin coronatine were 10- and 15-fold lower than those of the wild-type strain on soybean and on bean, and 16/83-E1 produced very weak symptoms compared with the wild-type strain. The coronatine-producing efe mutant 7a/90-E1 reached fourfold and twofold lower population sizes compared with the wild-type strain on soybean and bean, respectively, and caused disease symptoms typical of the wild-type strain. Experiments with ethylene-insensitive soybeans confirmed these results. The virulence of the wild-type strains was reduced to the same extent in ethylene-insensitive soybean plants as the virulence of the efe mutants in ethylene-susceptible soybeans. In contrast, the virulence of Pseudomonas syringae pv. phaseolicola was not affected by disruption of the efe gene.  相似文献   

The humeral immune response of swine after experimental infection with Salmonella typhimurium     

Steinbach G  Methner U  Springer S  Lindner T  Selbitz HJ 《Berliner und Münchener tier?rztliche Wochenschrift》2003,116(3-4):124-129

LPS antibody reaction of S. Typhimurium was detected in a total of 111 two- to ten-week-old pigs, which had been exposed to experimental oral infection with this serovar in 16 separate experiments. The total antibody level was determined according to the meat juice ELISA provided for the Salmonella control programme and the antibody concentrations of the isotopes IgG1, IgG2, IgA and IgM. Although there were significant differences between the individual experiments with respect to the reaction intensity and proportion of the individual immune globulin isotypes, the following general statements on the dynamics of the antibody reaction can be made: Clearly elevated antibody levels could be observed one week after the onset of infection. After one and two weeks, respectively, 22.5 and 52.9% of the infected animals showed antibody levels which were to be evaluated as positive in the sense of the Salmonella control programme (> or = 40%). While the concentration of the IgM-related antibodies decreased again during the second week already, the antibody concentration of IgG1 and IgG2 continued to increase until the end of the experiment after 3 weeks. The clear differences in the reactions between the experiments cannot be explained solely by defined conditions of infection such as strain of infection, infective dose or age of the pigs. Obviously, other non-defined or non-recognized factors may have a decisive influence on the dynamics of the antibody reaction in the animal groups.  相似文献   

Usefulness of serological examinations in the analysis of Salmonella infections in pig herds     

Steinbach G  Kröll U  Meyer H  Methner U 《Berliner und Münchener tier?rztliche Wochenschrift》2003,116(7-8):281-287

The development of the antibody concentration against lipopolysaccharide (LPS) of S. Typhimurium und S. Choleraesuis in rearing pigs during the fattening period and in breeding sows of the corresponding age was recorded. The studies revealed the following results. Antibodies of isotypes IgG1 and IgG2 revealed a more pronounced specificity against the according Salmonella serovar than IgM antibodies. The calculated "antibody percent value" based on the total amount of Salmonella antibodies is mainly determined by the IgM antibodies in sera and meat juice, respectively. In fattening pigs a significant increase of antibodies against IgM and total Ig was observed between week 3 and 10 after beginning of the rearing period. In breeding pigs this increase was detectable already earlier. In only 3 out of 10 groups an increase of IgG1 and IgG2 was also seen. The detected significant increase of total Ig and IgM in the other groups might be the result of a less intensive exposure to salmonellas or it might be due to an increase of unspecific antibodies induced by other antigens. Serological investigations represent a valuable tool to record the intensity and development in time of the Salmonella exposure in pigs farms. Examination of total Ig is an appropriate method to detect pig herds with a high level of Salmonella exposure, for detailed epidemiological studies in pig farms the examination of antibody isotypes will give more comprehensive information.  相似文献   

A single receptor binds both insulin-like growth factor II and mannose-6-phosphate   总被引：23，自引：0，他引：23  

R G MacDonald  S R Pfeffer  L Coussens  M A Tepper  C M Brocklebank  J E Mole  J K Anderson  E Chen  M P Czech  A Ullrich 《Science (New York, N.Y.)》1988,239(4844):1134-1137

Amino acid sequences deduced from rat complementary DNA clones encoding the insulin-like growth factor II (IGF-II) receptor closely resemble those of the bovine cation-independent mannose-6-phosphate receptor (Man-6-P receptorCI), suggesting they are identical structures. It is also shown that IGF-II receptors are adsorbed by immobilized pentamannosyl-6-phosphate and are specifically eluted with Man-6-P. Furthermore, Man-6-P specifically increases by about two times the apparent affinity of the purified rat placental receptor for 125I-labeled IGF-II. These results indicate that the type II IGF receptor contains cooperative, high-affinity binding sites for both IGF-II and Man-6-P-containing proteins.  相似文献   

The complete primary structure of protein kinase C--the major phorbol ester receptor   总被引：71，自引：0，他引：71  

P J Parker  L Coussens  N Totty  L Rhee  S Young  E Chen  S Stabel  M D Waterfield  A Ullrich 《Science (New York, N.Y.)》1986,233(4766):853-859

Protein kinase C, the major phorbol ester receptor, was purified from bovine brain and through the use of oligonucleotide probes based on partial amino acid sequence, complementary DNA clones were derived from bovine brain complementary DNA libraries. Thus, the complete amino acid sequence of bovine protein kinase C was determined, revealing a domain structure. At the amino terminal is a cysteine-rich domain with an internal duplication; a putative calcium-binding domain follows, and there is at the carboxyl terminal a domain that shows substantial homology, but not identity, to sequences of other protein kinase.  相似文献   

The neu gene: an erbB-homologous gene distinct from and unlinked to the gene encoding the EGF receptor   总被引：45，自引：0，他引：45  

A L Schechter  M C Hung  L Vaidyanathan  R A Weinberg  T L Yang-Feng  U Francke  A Ullrich  L Coussens 《Science (New York, N.Y.)》1985,229(4717):976-978

The neu oncogene, identified in ethylnitrosourea-induced rat neuroglioblastomas, had strong homology with the erbB gene that encodes the epidermal growth factor receptor. This homology was limited to the region of erbB encoding the tyrosine kinase domain. It was concluded that the neu gene is a distinct novel gene, as it is not coamplified with sequences encoding the EGF receptor in the genome of the A431 tumor line and it maps to human chromosome 17.  相似文献   

Texas entry-year agriculture teachers' perceptions, practices, and preparation regarding safety and health in agricultural education     

Hubert DJ  Ullrich DR  Murphy TH  Lindner JR 《Journal of agricultural safety and health》2001,7(3):143-153

The purpose of this study was to gather benchmark data for the assessment of the knowledge, attitudes, and perceptions regarding agricultural safety issues and curricula held by Texas agricultural teachers with less than two full years of teaching experience (entry-year teachers). Seventy-four of 118 well-distributed teachers responded to this survey. Researchers concluded that more females were entering a traditionally male-dominated field. Overall, teachers addressed safety within units of instruction rather than as separate units. The most useful forms of new teaching resources that this group of teachers would like to see produced were safety videos and study guides, and class demonstration/simulation activities. There was a significant difference in rankings between teachers less than 26 years old and teachers more than 26 years old regarding the usefulness of transparencies as a new teaching resource (F = 5.00, p = 0.0268). Few teachers were currently CPR and first aid certified, even though most had received training and completed a general safety and/or health related course while in college. Teachers generally agreed philosophically with most practices and exhibited personal beliefs consistent with proper safety preparedness and practice in agricultural settings. However, many of these teachers failed to practice what was expected of safe tractor operators, such as wearing safety belts and allowing younger drivers to operate the equipment.  相似文献   

The response of Mannheimia haemolytica to iron limitation: implications for the acquisition of iron in the bovine lung     

Roehrig SC  Tran HQ  Spehr V  Gunkel N  Selzer PM  Ullrich HJ 《Veterinary microbiology》2007,121(3-4):316-329

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Aspects of electrohydrodynamic instabilities at polymer interfaces     

Thomas P. Russell  Zhiqun Lin  Erik Schäffer  Ullrich Steiner 《Fibers and Polymers》2003,4(1):1-7

Electrospinning is emerging as a simple means of producing fibers with diameters ranging from 0.02μm to many microns. Electrospinning, however, relies on the force generated by an electric field on the surface of a polymer solution to either enhance instabilities in a thinning jet or to rapidly elongate a jet of polymer solution form a nozzle. In this article, the fundamental physics and fluid dynamics on the subject matter are described, and some of the relevant parameters in electrodynamic instabilities at polymer interfaces are discussed in some detail.  相似文献