全文获取类型
收费全文 | 73篇 |
免费 | 3篇 |
专业分类
林业 | 13篇 |
农学 | 1篇 |
基础科学 | 2篇 |
18篇 | |
综合类 | 4篇 |
农作物 | 5篇 |
水产渔业 | 1篇 |
畜牧兽医 | 25篇 |
植物保护 | 7篇 |
出版年
2022年 | 2篇 |
2021年 | 1篇 |
2020年 | 1篇 |
2019年 | 1篇 |
2018年 | 1篇 |
2016年 | 2篇 |
2014年 | 6篇 |
2013年 | 2篇 |
2012年 | 1篇 |
2011年 | 8篇 |
2010年 | 1篇 |
2009年 | 2篇 |
2008年 | 3篇 |
2007年 | 1篇 |
2006年 | 2篇 |
2005年 | 2篇 |
2004年 | 2篇 |
2003年 | 3篇 |
2002年 | 3篇 |
2001年 | 3篇 |
2000年 | 4篇 |
1999年 | 1篇 |
1998年 | 2篇 |
1997年 | 1篇 |
1996年 | 3篇 |
1995年 | 3篇 |
1994年 | 1篇 |
1991年 | 1篇 |
1990年 | 3篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1987年 | 2篇 |
1985年 | 2篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1977年 | 1篇 |
1972年 | 1篇 |
排序方式: 共有76条查询结果,搜索用时 31 毫秒
1.
V. Villar B. Colao I. Calles‐Venal J. G. Fernndez‐lvarez M. Fernndez‐Caso J. M. Villar 《Anatomia, histologia, embryologia》2005,34(Z1):54-54
Chondrocytes dedifferentiate to a fibroblast‐like phenotype on plastic surfaces. Dedifferentiation is reversible if these cells are then cultured embedded in gels as alginate, agarose or collagen. Chondrocytes cultured in suspension on a non‐adherent surface are also known to form aggregates of differentiated cells. The knowledge of chondrocyte behavior in culture is relevant for tissue engineering purposes. In this report we describe a simple method to culture differentiated or redifferentiated rabbit auricular chondrocytes on plastic surfaces with a stable phenotype. When chondrocyte aggregates formed in suspension are next seeded on plastic surfaces, most of them attach to the plastic as round or polygonal cells, and this morphological differentiation, confirmed by the presence of type II collagen, is stable for long culture periods. We also report that the addition of aggregates to monolayer cultures of dedifferentiated chondrocytes results in their redifferentiation, as is shown by their morphological changes and the synthesis of type II collagen. Therefore, this simple method can be useful for the study of chondrocyte behavior on plastic surfaces and for redifferentiating previously proliferated chondrocytes in tissue engineering techniques. Furthermore, these results demonstrate that, in addition to culture conditions such as cell isolation method or cell‐density, chondrocyte behavior on plastic depends on the presence or absence of aggregates resulting from the dissociation process. 相似文献
2.
Kenaf (Hibiscus cannabinus L.) is an annual plant which produces a very high fiber yield per hectare and has demonstrated to be a suitable material for
pulping. The crop conditions influence fiber production, but there is no information about their effect on pulp quality. The
aim of this work was to study the effect of kenaf varieties and crop conditions (watering dose and harvesting time) on sulfate
pulp quality. The study has been made on bast fiber sulfate pulps due to their high added value. The quality criteria were
fiber dimensions, chemical composition and pulp quality. Kenaf variety or crop conditions have significant effect on fiber
dimensions, holocellulose content, kappa number and breaking strength. To obtain high fiber yield and good delignified pulps
it is recommended to grow the Salvador variety and to employ advance harvesting.
Received: 20 January 1999 相似文献
3.
4.
在人工接种和自然感虫情况下美洲黑杨后代对桑天牛抗性的研究 总被引:5,自引:0,他引:5
杨树 (Populusspp .)是我国主要造林和工业用材树种之一 ,据统计我国杨树人工林面积达 666×1 0 4hm2 (王世绩 ,1 995 )。但杨树天牛危害十分猖獗 ,其中光肩星天牛 (Anoplophoraglabripennis)和黄斑星天牛 (Anoplophoranobi 相似文献
5.
Javier Fernández Laura Marín Raquel álvarez-Alonso Saúl Redondo Juan Carvajal Germán Villamizar Claudio J. Villar Felipe Lombó 《Marine drugs》2014,12(5):2668-2699
Diverse actinomycetes produce a family of structurally and biosynthetically related non-ribosomal peptide compounds which belong to the chromodepsipeptide family. These compounds act as bisintercalators into the DNA helix. They give rise to antitumor, antiparasitic, antibacterial and antiviral bioactivities. These compounds show a high degree of conserved modularity (chromophores, number and type of amino acids). This modularity and their high sequence similarities at the genetic level imply a common biosynthetic origin for these pathways. Here, we describe insights about rules governing this modular biosynthesis, taking advantage of the fact that nowadays five of these gene clusters have been made public (thiocoraline, triostin, SW-163 and echinomycin/quinomycin). This modularity has potential application for designing and producing novel genetic engineered derivatives, as well as for developing new chemical synthesis strategies. These would facilitate their clinical development. 相似文献
6.
Victoria González-Rodríguez Rafael Villar Raquel Casado Elena Suárez-Bonnet José Luis Quero Rafael M. Navarro-Cerrillo 《Annals of Forest Science》2011,68(7):1217-1232
Introduction
The great spatial and temporal heterogeneity of Mediterranean ecosystems can influence establishment success in woody species, whose natural regeneration occurs to a very small extent. In this work, the effect of the spatial pattern of environmental variables (light availability, soil moisture and herbaceous production) on seedling emergence, growth, survival and establishment success was examined by using a spatially explicit approach. 相似文献7.
F. J. Vázquez V. Petrikova M. C. Villar T. Carballas 《Biology and Fertility of Soils》1996,22(3):265-271
Annual (Pisum sativum L. and Vicia sativa L.) and perennial (Trifolium repens L. and Lotus corniculatus L.) leguminous species were grown in pots containing samples from the ash layers of two Cambisols under Pinus sylvestris L., which has been affected by high-intensity wildfires 3 and 15 days before the sampling. The gramineous Lolium perenne L. was cultivated as a second plant after Trifolium and Lotus harvesting. Three treatments were compared: soils without fertilization and soils fertilized with two doses of poultry manure (1 and 2 g total N kg-1 dry soil). The aim of the work was to study the capacity of the ash layer to sustain vegetation and the influence of plants and organic manure on the recovery of vegetation cover, ash layer fixation and soil structure formation to avoid erosion. The ash samples were able to sustain vegetation without fertilization. The organic manure increased the yields of all the plants tested, the lower dose being the optimal for the first crop whereas the higher dose was beneficial for the second crop. The annual legumes grew very quickly. The mixture of Trifolium and Lotus seemed very suitable for reclamation of soil degraded by wildfires because Trifolium produced more phytomass than Lotus in the first growing stages whereas the development of Lotus was higher in the later growing stages. Ash layer conditions did not inhibit nodulation, which was, however, stimulated by the organic manure, particularly in the case of Lotus. Lolium after perennial legumes was the best plant combination because it produced the highest phytomass, particularly root phytomass, and thus improved vegetation cover and ash layer fixation. All the plants tested improved the formation of soil aggregates, particularly the combination of perennial legumes and Lolium. However, wet aggregate stability was higher when plants were grown on soils fertilized with poultry manure than when plants were cropped on unmanured soils, which points to the favourable influence of the organic manure on soil aggregation. 相似文献
8.
Expression of beta-nerve growth factor receptor mRNA in Sertoli cells downregulated by testosterone 总被引:4,自引:0,他引:4
H Persson C Ayer-Le Lievre O S?der M J Villar M Metsis L Olson M Ritzen T H?kfelt 《Science (New York, N.Y.)》1990,247(4943):704-707
Nerve growth factor (NGF) is synthesized in male germ cells. The NGF receptor (NGFR) mRNA was found in the Sertoli cells of rat testis. Hypophysectomy increased both NGFR mRNA in testis and the number of NGFR hybridizing cells in seminiferous tubules. This was suppressed by treatment with chorionic gonadotropin or testosterone, but not with follicle-stimulating hormone. The NGFR mRNA also increased after destruction of Leydig cells or blocking of the androgen receptor. This suggests that NGF produced by male germ cells regulates testicular function in an androgen-modulated fashion by mediating an interaction germ and Sertoli cells. 相似文献
9.
Two complex profiles in slope deposits in Galicia, Spain, were examined both in the field and in the laboratory. One profile consists of humous colluvium on solifluction material overlying gabbro, the other shows various layers of humous collovium on granite. 相似文献
10.
Aurelia Bonet Nieves Diaz-Caneja Isabel Gutierrez Antonio Martínez Eugenio Villar 《Water, air, and soil pollution》1988,38(1-2):181-188
A study was carried out on the pH values obtained from bulk (wet and dry) precipitation samples collected simultaneously in two cities in Cantabria (Spain), Santander and Torrelavega over a period of a year. It was observed that the annual weighted average values for the pH of precipitation, 4.42 and 4.21, respectively, differ significantly although the difference is less than that existing between their pollution levels. The difference is greater when total annual H+ depositions are compared. The study also demonstrated the strong influence of long-range transport effect on acidity and to a lesser degree the effect of mid-range transport. 相似文献