Development and evaluation of an indirect enzyme-linked immunosorbent assay for detection of foot-and-mouth disease virus nonstructural protein antibody using a chemically synthesized 2B peptide as antigen.     

Toru Inoue  Satya Parida  David J Paton  Wilai Linchongsubongkoch  David Mackay  Yooni Oh  Dilok Aunpomma  Simon Gubbins  Takakiyo Saeki 《Journal of veterinary diagnostic investigation》2006,18(6):545-552

Forty peptides were synthesized corresponding to hydrophilic clusters of amino acids within the sequences of foot-and-mouth disease virus (FMDV) nonstructural proteins (NSP). Six peptides were studied in more detail and the most promising, a 2B peptide, was evaluated in enzyme-linked immunosorbent assay (ELISA) using sera from naive, vaccinated, and vaccinated-and-challenged cattle as well as bovine sera from field outbreaks. The performance of the new NSP peptide ELISA was compared to that of 4 commercial NSP ELISA kits. Antibody to 2B was detectable from the end of the first week to the second week after infection in most of the nonvaccinated animals and by the second to third week in vaccinated-and-challenged animals. The sensitivity of the 2B peptide ELISA was comparable to the 3ABC Ceditest (Ceditest FMDV-NS, Cedi Diagnostics B.V.; Chung et al., 2002). With some modification and further validation, this 2B test could be useful as a screening or conformational NSP test in postvaccination surveillance for FMD.  相似文献   

Development and validation of a 3ABC antibody ELISA in Australia for foot and mouth disease     

A Colling  C Morrissy  J Barr  G Meehan  L Wright  W Goff  LJ Gleeson  B van der Heide  S Riddell  M Yu  D Eagles  R Lunt  S Khounsy  Ngo Than Long  Pham Phong Vu  Nguyen Than Phuong  Nguyen Tung  W Linchongsubongkoch  J Hammond  M Johnson  WO Johnson  H Unger  P Daniels  JR Crowther 《Australian veterinary journal》2014,92(6):192-199

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