Detection of catecholamines and metanephrines by radio-immunoassay in canine plasma     

Roland C.E. Francis  Philipp A. Pickerodt  Lothar Salewski  Willehad Boemke  Claudia Höhne 《Veterinary journal (London, England : 1997)》2010,183(2):228-231

This study investigated the applicability of two human radio-immunoassays (RIA) to detect epinephrine (EPI), norepinephrine (NE), and their O-methylated metabolites metanephrine (MN) and normetanephrine (NMN) in canine plasma. The analysis yielded a positive correlation between metabolites and their respective parent compounds: EPI and MN (r = 0.63), NE and NMN (r = 0.47), as well as between parent compounds, EPI and NE (r = 0.48), and between metabolites MN and NMN (r = 0.71). Moreover, EPI (r = 0.99) and NE (r = 0.77) concentrations determined by RIA did correlate positively with high pressure liquid chromatography (HPLC). However, there was limited agreement between both methods. It was concluded that complete validation tests for accuracy, precision and agreement are needed before this RIA can be applied to quantify catecholamines, metanephrine, and normetanephrine in canine plasma. The assay may prove to be a potential alternative to HPLC or tandem mass spectrometry in the work-up of pheochromocytoma and the detection of overall sympathetic activity in dogs.  相似文献   

Endothelin receptor subtype A blockade does not affect the haemodynamic recovery from haemorrhage during xenon/remifentanil or isoflurane/remifentanil anaesthesia in dogs     

Roland CE Francis  Claudia Höhne  Adrian Klein  Udo X Kaisers  Philipp A Pickerodt  Willehad Boemke 《Veterinary anaesthesia and analgesia》2010,37(3):258-268

ObjectiveTo test the compensatory role of endothelin-1 when acute blood loss is superimposed on anaesthesia, by characterizing the effect of systemic endothelin receptor subtype A (ET_A) blockade on the haemodynamic and hormonal responses to haemorrhage in dogs anaesthetized with xenon/remifentanil (X/R) or isoflurane/remifentanil (I/R).Study designProspective experimental randomized controlled study.AnimalsSix female Beagle dogs, 13.4 ± 1.3 kg.MethodsAnimals were anaesthetized with remifentanil 0.5 μg kg^?1 minute^?1 plus either 0.8% isoflurane (I/R) or 63% xenon (X/R), with and without (Control) the systemic intravenous endothelin receptor subtype A antagonist atrasentan (four groups, n = 6 each). After 60 minutes of baseline anaesthesia, the dogs were bled (20 mL kg^?1) over 5 minutes and hypovolemia was maintained for 1 hour. Continuous haemodynamic monitoring was performed via femoral and pulmonary artery catheters; vasoactive hormones were measured before and after haemorrhage.ResultsIn Controls, systemic vascular resistance (SVR), vasopressin and catecholamine plasma concentrations were higher with X/R than with I/R anaesthesia at pre-haemorrhage baseline. The peak increase after haemorrhage was higher during X/R than during I/R anaesthesia (SVR 7420 ± 867 versus 5423 ± 547 dyne seconds cm^?5; vasopressin 104 ± 23 versus 44 ± 6 pg mL^?1; epinephrine 2956 ± 310 versus 177 ± 99 pg mL^?1; norepinephrine 862 ± 117 versus 195 ± 33 pg mL^?1, p < 0.05). Haemorrhage reduced central venous pressure from 3 ± 1 to 1 ± 1 cmH₂O (I/R, ns) and from 8 ± 1 to 5 ± 1 cmH₂O (X/R, p < 0.05), but did not reduce mean arterial pressure, nor cardiac output. Atrasentan did not alter the haemodynamic and hormonal response to haemorrhage during either anaesthetic protocol.Conclusions and clinical relevanceSelective ET_A receptor blockade with atrasentan did not impair the haemodynamic and hormonal compensation of acute haemorrhage during X/R or I/R anaesthesia in dogs.  相似文献