Association of neutrophil morphology with bacterial isolates in equine tracheal wash samples          下载免费PDF全文

N. A. Jocelyn  C. E. Wylie  M. Lean  A. Barrelet  A. K. Foote 《Equine veterinary journal》2018,50(6):752-758

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Arthroscopy of the Dorsal and Plantar Pouches of the Tarsocrural Joint for the Treatment of Osteochondritis Dissecans in the Horse: Clinical Features and Follow‐Up          下载免费PDF全文

Oliver James  Richard Payne  Andrew Bathe  Tim Greet  Claire Wylie 《Veterinary surgery : VS》2016,45(8):1049-1055

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Pronuclear Embryo Yield in Canindé and Saanen Goats for DNA Microinjection     

RR Moura  ES Lopes‐Junior  DIA Teixeira  IA Serova  LE Andreeva  LM Melo  VJF Freitas 《Reproduction in domestic animals》2010,45(5):e101-e106

The objective of this study was to examine the effect of donor breed on pronuclear‐stage embryo yield to be used for DNA microinjection in a transgenesis goat program. Twelve Canindé and twelve Saanen goats were heat synchronized using a progestagen‐cloprostenol treatment. Forty‐eight hours before the sponge removal, superovulation was induced with a total administration of 4.4 mg/kg bodyweight NIH‐FSH‐P1, given twice daily in decreasing doses over 3 days. In addition, goats received 100 μg of GnRH and they were hand‐mated at 36 and 48 h after progestagen removal. Embryo recovery was performed by oviduct flushing at 72 h after sponge removal. Embryos were microinjected with a DNA construct and noticeable swelling of the nuclei was the criterion for successful microinjection. The total diameter, cytoplasm diameter, zona pellucida thickness and pronuclei diameter were measured for each microinjected embryo. A higher (p < 0.05) percentage of fertilized ova was observed in Canindé (89.9%) than Saanen (36.2%) goats. In addition, Canindé donors produced a higher percentage of pronuclear embryos when compared with Saanen: 72.5% vs 20.6% (p < 0.05), respectively. Successful microinjection was verified in 96.7% and 73.3% of times in Canindé and Saanen embryos, respectively (p < 0.05). Significant differences were observed for all morphometric parameters except for cytoplasm diameter. In conclusion, under our study experimental conditions, Canindé were more efficient than Saanen goats concerning the pronuclear embryo yield and manipulation. The use of Canindé goats in transgenesis programs could be increase the interest in their breeding and could be contribute to saving them from extinction.  相似文献   

Effects of ambient temperature and restricted feeding on the growth of feathers in growing turkeys   总被引：2，自引：0，他引：2  

Wylie LM  Robertson GW  Macleod MG  Hocking PM 《British poultry science》2001,42(4):449-455

1. Male turkeys were reared to 6 weeks of age at 15 degrees C and 26 degrees C and fed ad libitum or restricted to 0.5 of the body weight of birds fed ad libitum. Basal metabolic rate was determined by indirect calorimetry at an ambient temperature of 20 degrees C. 2. Turkeys at 15 degrees C were lighter than those kept at 26 degrees C. Feather lengths and weight were similar in both groups. Fasting heat production corrected for both metabolic body size and activity was greater in turkeys reared at 15 degrees C than those at 26 degrees C. 3. Cranial breast feathers were significantly longer in restricted birds than in those fed ad libitum in contrast to a proportional decrease in the lengths of other feathers of 0.1 to 0.3. Feather weight as a proportion of body weight was 0.072 in restricted turkeys compared with 0.046 in birds fed ad libitum. There was no difference in basal metabolic rate between ad libitum and restricted turkeys. 4. It was concluded that feather growth was maintained in preference to body and muscle growth and that rearing birds at 15 degrees C did not improve breast feather cover. It is suggested that the growth of breast feathers in turkeys fed ad libitum is impaired.  相似文献   

Fertility of dairy cows in Northern Ireland     

Mayne CS  McCoy MA  Lennox SD  Mackey DR  Verner M  Catney DC  McCaughey WJ  Wylie AR  Kennedy BW  Gordon FJ 《The Veterinary record》2002,150(23):707-713

A comprehensive database was established on the milk production and reproductive performance of dairy cows in 19 selected herds in Northern Ireland, varying in size, management system and genetic merit. Data were obtained for 2471 cows, 1775 of which calved in a second year, and 693 were culled from the herd for specific reasons. The estimated mean rate of heat detection (assessed by the interheat interval during the main breeding season) in all the herds was 71 per cent, with a range from 53 to 92 per cent The average conception rate to first insemination was 37.1 per cent (range 21 to 66 per cent). The average calving interval for the retained cows was 407.2 days (range 359 to 448 days). Twenty-eight per cent of the cows that calved were culled, with infertility being the largest single reason (26.8 per cent of the cows culled). There were major differences in reproductive performance between the herds, but heat detection rate, conception rate and calving interval did not appear to be affeded by a herd's genetic merit. The herds with shorter calving intervals were characterised by better heat detection efficiency (83 v 61 per cent, P<0.01), a shorter interval from calving to first insemination (74 v 97 days; P<0.05), a higher conception rate to first insemination (45 v 34 per cent, P>0.10) and a lower removal rate (23 v 37 per cent, P<0.01). Furthermore, the cows in these herds had lower body condition scores (BCS) in the dry period (3.0 v 3.3; P<0.05) but lost less body condition in early lactation (0.3 v 0.6 BCS units, P<0.05). These results show that dairy herd fertility in Northern Ireland is generally low and similar to that previously reported for England and the USA, but that in some herds changes in herd management practices improved the cows' fertility.  相似文献   

Seed dormancy and germination in the summer annual Galeopsis speciosa     

LM KARLSSON  JAL ERICSSON  & P MILBERG 《Weed Research》2006,46(5):353-361

This study examined germination and dormancy in Galeopsis speciosa (Lamiaceae), a common summer annual weed in cold‐temperate areas. Seeds collected in southern Sweden were subjected to several experiments. The seeds were dormant at maturity. Seeds sown outdoors after collection produced a small number of seedlings that emerged early in the spring. After long cold stratification or stratification outdoors over two winters, the maximum germination was 40–50%; germination occurring over a wide range of temperatures. Warm stratification preceding cold stratification had no effect on germination, but repeated warm and cold periods seemed to promote germination. Gibberellic acid (GA) stimulated germination, but full germination was only achieved after more than 2 months of incubation at the most suitable temperature regime tested. Excised embryos grew and developed into normal seedlings. With these results, the species does not fit into the currently used system for seed dormancy classifications. The response to GA and the growth of excised embryos indicate non‐deep or intermediate physiological dormancy, but dormancy alleviation by stratification was not in line with the guiding principles for these classifications. Galeopsis speciosa has a strong dormancy that is sufficiently alleviated during the winter to allow germination of only part of a seed batch each year; hence a stepwise germination pattern occurs over a period of several years.  相似文献   

The Equine Manual     

LM Begg 《Australian veterinary journal》2006,84(8):300-300

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Virus impact at the interface of an ancient ecosystem and a recent agroecosystem: studies on three legume-infecting potyviruses in the southwest Australian floristic region     

C. G. Webster  B. A. Coutts  R. A. C. Jones  M. G. K. Jones  S. J. Wylie 《Plant pathology》2007,56(5):729-742

The southwest Australian floristic region (SWAFR) is an internationally recognized 'hot spot' of global biodiversity and has an endangered flora. It represents a unique interface between an ancient ecosystem and a recent agroecosystem, providing the opportunity to investigate encounters where the recipient of the virus is an introduced crop and the donor a native plant and vice versa. Phylogenetic analysis of the virus coat-protein genes was used to study isolates of three potyviruses representing different 'new encounter' scenarios at this interface. The incidence, symptomatology, host range, non-persistent aphid transmission and considerable genetic diversity of the first indigenous virus described from the SWAFR, where it infects the native legume Hardenbergia comptoniana , and its potential to damage lupin, a locally important, newly introduced cultivated grain legume, was studied. The name Hardenbergia mosaic virus is proposed for this virus. Two other examples of 'new encounter' scenarios involving other legume-infecting potyviruses studied were: Passion fruit woodiness virus , which has been found only in Australasia, where it damages recently introduced species of Passiflora and legumes; and Bean yellow mosaic virus, which is not indigenous to Australia and was introduced recently to the SWAFR, where it infects a number of introduced legumes, but also damages the local native legume Kennedia prostrata . Isolates of the former had considerable genetic diversity consistent with the virus being indigenous, while isolates of the latter virus from K. prostrata had a low genetic diversity consistent with recent arrival. This research illustrates how introduced viruses can damage indigenous plants and indigenous viruses can damage introduced cultivated plants within this unique ecosystem, and how human activities can facilitate damaging 'new encounters' between plants and viruses.  相似文献   

THE PROGRAM OF THE UNIVERSITY ASSOCIATION FOR THE STUDY OF CALENDAR REFORM     

Wylie CC 《Science (New York, N.Y.)》1931,73(1900):587-588

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WHERE DO METEORITES COME FROM?     

Wylie CC 《Science (New York, N.Y.)》1939,90(2334):264-265

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