Chromosomal and Plasmid Diversity of Agrobacterium Strains Isolated from Ficus benjamina Tumors     

Aida Raio  Raffaele Peluso  Xavier Nesme  Astolfo Zoina 《European journal of plant pathology / European Foundation for Plant Pathology》2004,110(2):163-174

Agrobacteria were previously isolated from tumors developing on branches and aerial and hypogeous roots of weeping fig plants in Italy and in The Netherlands. A representative group of 48 strains was analyzed by PCR–RFLP of 16S and 16S + IGS ribosomal regions, PCR–RFLP of six Ti plasmid (pTi) regions and characterized for plasmid content. Two groups of agrobacteria were separated by cluster analysis of PCR–RFLP profiles of rrs gene: seventeen strains were similar to the new species Agrobacterium larrymoorei, while the remaining strains were included within the agrobacterium biovar 1 group. Sixteen different plasmid profiles from one to five plasmids were observed. In addition, 21 ribotypes and 20 pTi structures were arranged in many different combinations, showing that fig agrobacteria were characterized by a wide heterogeneity. A general lack of correlation between strain ribotypes and plasmid content was observed.  相似文献   

Laboratory tests for diagnosing and monitoring canine leishmaniasis          下载免费PDF全文

Saverio Paltrinieri  Luigi Gradoni  Xavier Roura  Andrea Zatelli  Eric Zini 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2016,45(4):552-578

Although several reviews on canine leishmaniasis have been published, none thoroughly described clinicopathologic abnormalities and their clinical usefulness. The aim of this review was to provide information concerning current diagnostic tests relevant for clinical pathologists and from a practical perspective. Specifically, in canine leishmaniasis, nonregenerative normocytic normochromic anemia, thrombocytopenia, or leukogram changes may be present. Clinical chemistry and urinalysis may indicate renal dysfunction (azotemia, decreased urine specific gravity, proteinuria) and an inflammatory/immune response (increased acute phase proteins [APP] or α₂‐ and/or γ‐globulins). Although a potential gammopathy is usually polyclonal, it may also appear oligo‐ or monoclonal, especially in dogs coinfected by other vector‐borne pathogens. When lesions are accessible to fine‐needle aspiration (lymphoadenomegaly, nodular lesions, joint swelling), cytology is strongly advised, as the presence of Leishmania amastigotes in a pattern of pyogranulomatous inflammation or lymphoplasmacytic hyperplasia is diagnostic. If the cytologic pattern is inconclusive, the parasite should be identified by histology/immunohistochemistry or PCR on surgical biopsies. Alternatively, cytology and PCR may be performed on bone marrow samples where amastigotes, along with erythroid hypoplasia, myeloid hyperplasia, plasmacytosis, or secondary dysmyelopoiesis can be observed. Dogs with overt leishmaniasis generally have high antibody titers, while low titers predominate in immunologically resistant infected dogs or in exposed dogs with no parasite confirmation. Quantitative serology is recommended in clinically suspect dogs as high‐titer antibodies titers may confirm the clinical diagnosis. In confirmed and treated dogs, renal function and inflammatory/immune response variables should be periodically monitored.  相似文献   

Field evaluation of glutathione and glutamic acid as host marking pheromones for control of Tephritid fruit flies in a mango orchard in Kenya     

Xavier Cheseto  Hillary Kirwa  Samira Abuelgasim Mohamed  Sunday Ekesi  John J Beck  Baldwyn Torto 《Pest management science》2023,79(4):1585-1592

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Effects of irrigation regimes on the yield and water use of strawberry     

Lydia Serrano  Xavier Carbonell  Robert Savé  Oriol Marfà  Josep Peñuelas 《Irrigation Science》1992,13(1):45-48

Summary Strawberry plants (Fragaria x annanasa D. cv Chandler) were grown in field plots and in drainage lysimeters under controlled soil moisture regimes. Four irrigation treatments were established by watering the plants when soil water potential reached -0.01, -0.03,-0.05 and -0.07 MPa. The maximum yield was attained at -0.01 MPa soil water potential. Differences in yield were caused by both changes in the number of fruits per plant and in the fresh weight per fruit. Yield reductions were associated with reductions in total assimilation rate resulting from the decreased assimilatory surface area in plants irrigated at lower soil water potentials. The crop water production function calculated on a fruit fresh weight basis resulted in a yield response factor (K _y) of 1.01.  相似文献   

Effect of immunosuppression on clinical and pathologic manifestations of Marek's disease in chickens   总被引：1，自引：0，他引：1  

A G Foster  T Moll 《American journal of veterinary research》1968,29(9):1831-1835

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Purulent pericarditis as a sequela to clostridial myositis in a horse     

May KA  Cheramie HS  Howard RD  Duesterdieck K  Moll HD  Pleasant RS  Pyle RL 《Equine veterinary journal》2002,34(6):636-640

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育雏前期日粮中的核苷酸对雏鸡的促生长作用     

Fernando Rutz  Patrícia Rossi  刘蓓一 《中国家禽》2007,29(15):34-36

雏鸡的第1周的饲料数量仅是上市鸡总饲料的3.5%,但是为了得到最适的生长性能,环境的管理和恰当的营养是关键.因为雏鸡和成年鸡不一样,雏鸡没有复杂的消化酶,所以不能很好的起到消化的作用.在育雏期的早期,雏鸡面临着最困难的生理过渡时期,也就是开产期日粮中复杂的碳水化合物、蛋白质、脂类替代了胚胎中脂类和蛋白质的营养来源.而且雏鸡的免疫系统发育不完全,免疫主要靠母源抗体.……  相似文献   

Major gene resistance in Brassica napus (oilseed rape) is overcome by changes in virulence of populations of Leptosphaeria maculans in France and Australia     

Susan J. Sprague  Marie-Hélène Balesdent  Hortense Brun  Helen L. Hayden  Stephen J. Marcroft  Xavier Pinochet  Thierry Rouxel  Barbara J. Howlett 《European journal of plant pathology / European Foundation for Plant Pathology》2006,114(1):33-40

Resistance of Brassica napus (oilseed rape, canola) conferred by three different major resistance genes has been overcome by changes in virulence of Leptosphaeria maculans populations in France and Australia. In South Australia where B. napus cultivars with major gene resistance derived from Brassica rapa ssp. sylvestris were grown extensively, resistance was rendered ineffective within 3 years of commercial release of the cultivar. Disease severity was higher on cultivars with sylvestris-derived resistance than cultivars with polygenic resistance. This Australian situation is compared to that in France, where resistance conferred by the Rlm1 gene was overcome nation-wide in 5 years under commercial cropping practices, and also where a source of resistance introgressed into B. napus from B. juncea was rendered inefficient in 3 years in experimental field plots near Rennes.  相似文献   

Detection of Leishmania spp. and associated inflammation in ocular‐associated smooth and striated muscles in dogs with patent leishmaniosis     

Carolina Naranjo  Dolors Fondevila  Marta Leiva  Xavier Roura  Teresa Pea 《Veterinary ophthalmology》2010,13(3):139-143

Objective Canine leishmaniosis is a disease characterized by the wide distribution of the parasite throughout the tissues of the host. The purpose of this study was to describe the presence of Leishmania spp. and associated inflammation in ocular‐associated muscles of dogs with patent leishmaniosis. Procedures Smooth muscles (iris dilator muscle, iris sphincter muscle, ciliary muscle, Müller muscle, smooth muscle of the periorbita and smooth muscle of the nictitating membrane) and striated muscles (orbicularis oculi muscle, obliquus dorsalis muscle and dorsal rectus muscle) were evaluated. Routine staining with hematoxylin and eosin and immunohistochemistry to detect Leishmania spp. were performed on tissue sections. Results Granulomatous inflammation was seen surrounding muscular fibers and was composed mainly of macrophages with scattered lymphocytes and plasma cells. This infiltrate could be seen in 52/473 (10.99%) samples of smooth muscle and 36/142 (25.35%) samples of striated muscle. Parasites were detected in 43/473 (9.09%) samples of smooth muscle and in 28/142 (19.71%) samples of striated muscle. Conclusions To the authors’ knowledge, this is the first report assessing the presence of Leishmania spp. and associated infiltrate in intraocular, extraocular and adnexal smooth and striated muscles. The inflammation present in those muscles could contribute to clinical signs already described, such as blepharitis, uveitis, and orbital cellulitis.  相似文献   

Von Holzkonservierung     

Dr. Dr. Ing. Friedrich Moll 《European Journal of Forest Research》1925,47(3):225-230

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