Cryptosporidiosis is caused by species of Cryptosporidium protozoa that can infect a wide range of host animals worldwide. However, data regarding Cryptosporidium infection in farmed pigs in subtropical areas in China is limited. Therefore, a total of 396 fecal samples were obtained from farmed pigs from Zhejiang (n?=?124), Guangdong (n?=?72), and Yunnan (n?=?200) provinces, China, and were tested by PCR amplification of the small subunit (SSU) rRNA gene. The overall prevalence of Cryptosporidium in pigs was 17.68% (70/396), with 20.11% (36/179) in male pigs and 15.67% (34/217) in female pigs. Additionally, Cryptosporidium prevalence was 8.33% (6/72) in Guangdong province, 14.52% (18/124) in Zhejiang province, and 23.00% (46/200) in Yunnan province. A DNA sequence analysis of the SSU rRNA gene revealed that all of the isolates represented C. scrofarum. This is the first report of C. scrofarum infection in pigs in Guangdong and Yunnan provinces in subtropical areas of China. The results of the present study provide foundation data for control and prevention of Cryptosporidium infection in pigs in the study areas in China.
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Dong-Hui Zhou Fu-Rong Zhao Hui-Yan Xia Min-Jun Xu Si-Yang Huang Hui-Qun Song Xing-Quan Zhu 《Irish veterinary journal》2013,66(1):1-4
Chlamydia spp. are obligate intracellular gram-negative bacteria that cause a wide range of significant diseases in humans and animals worldwide, resulting in significant economic losses. Chlamydial infection in cattle has been reported in many countries including China. However, there has been no survey of chlamydial infection of dairy cattle in Guangzhou, southern China. The objective of the present investigation was to examine the chlamydial seroprevalence in dairy cattle in Guangzhou, subtropical southern China by using an indirect hemagglutination assay (IHA). The overall seroprevalence of chlamydial infection in dairy cattle was 7.25% (29/400). Greater than or equal to eight-yr-old dairy cattle had the highest seroprevalence (10.34%), followed by those that were ≥ 6 years old or < 7 years old dairy cattle (10.20%), although there were no statistically significant differences among different groups (P > 0.05). Dairy cattle with 5 pregnancies had the highest seroprevalence (10.81%). These results indicate that chlamydial infection was present in dairy cattle in Guangzhou, subtropical southern China, and integrated strategies and measures should be executed to control and prevent chlamydial infection and disease outbreak in the study region. 相似文献
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农业生态系统健康及其管理研究进展 总被引：5，自引：0，他引：5
生态系统健康是生态系统管理的目标。阐述了农业生态系统健康的概念、评价指标和评价方法,指出农业生态系统健康评价指标与标准的选择应根据其结构和功能将社会经济指标及资源环境指标有机结合,以客观评价农业环境资源承载力及农业生产发展方向,并科学管理农业生态系统。 相似文献
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Guo-Hua Liu Dong-Hui Zhou Wei Cong Xiao-Xuan Zhang Xin-Chun Shi Ciren Danba Si-Yang Huang Xing-Quan Zhu 《Tropical animal health and production》2014,46(1):257-259
Swine influenza A virus (SIV) is zoonotic pathogen that can be acquired by food-borne transmission because food animals, for example pigs, are recognized as a reservoir. The objectives of this study were to determine the seroprevalence of anti-SIV (H1N1 and H3N2) in Tibetan pigs in Tibet Nationality Autonomous Region, China, a region with cold weather and high altitude. A total of 421 serum samples were randomly collected from Tibetan pigs in Tibet and were evaluated for antibodies against SIV using enzyme-linked immunosorbent assay. Overall, 52 % (219/421) of the animals was positive for H1N1, 16.9 % (71/421) positive for H3N2, and 8.8 % (37/421) positive for both H1N1 and H3N2. The results of the present survey indicated that SIV is highly prevalent among Tibetan pigs in Tibet, China. The results of the present investigation have implications for the ongoing control of SIV infection in Tibetan pigs in Tibet, China and elsewhere. 相似文献
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利用1D-SDS-PAGE分离了261份青藏高原农家青稞的淀粉颗粒结合蛋白,旨在为青藏高原青稞淀粉品质改良和淀粉颗粒结合蛋白机制研究提供依据和基础信息。在分子量45~100 kD区域共有20种多态性蛋白条带和78种组合带型,其中2、3、5、10、11为新条带。利用PCR技术克隆了236份农家青稞GBSSI基因5′前导序列,出现1 000 bp和800 bp的多态性片段,且以前者为主,其频率为80.1%。在8份农家青稞及4份引进的低直链淀粉材料的GBSSI基因5′前导序列中共检测到32个多态性位点,包括9个InDel和23个SNP。GBSSI基因5′前导序列中出现了特有的序列差异,如未出现600 bp类型(约400 bp的特异缺失),而该缺失被认为是低直连淀粉大麦形成的原因;材料yf127、yf70、011Z1396和09Z586出现了特异点突变。因此认为,青藏高原农家青稞品种的淀粉颗粒结合蛋白具有丰富的多态性和独特性,可能存在新的形成机制。 相似文献
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水稻小粒不育系新组合卓两优141混播制种优势分析 总被引：1，自引：0，他引：1
传统的杂交水稻制种方式落后、效益低、成本高的问题,制约了杂交稻推广应用。利用小粒型不育系可实现杂交水稻混播、混收、机械分离等全程机械化制种,降低杂交稻制种成本,提高制种效率。本研究以小粒型不育系卓201S、大粒恢复系R141和新组合卓两优141为材料,对其适合机械化制种的农艺性状、异交特性、混播制种及大面积制种实践进行了研究。结果表明,与C815S相比,卓201S株高较矮,穗长较长且直立,包颈粒率、穗萌率、黑粉病、开颖率均低于C815S,对"九二〇"较敏感,且较难落粒,具有较好的农艺性状。而R141株高较高,花粉数量大,花期长,且对环境温度较钝感,两者均具有良好的异交特性。卓201S谷粒厚1.71mm,千粒重14.00g,R141谷粒厚2.23 mm,千粒重28.20 g,两者籽粒大小差异显著,通过特定的狭长形筛孔筛子,实现了杂种F1与父本种子的高效分离,杂交种子含父本率为0,杂种损失率为2.31%,种子纯度在生产上达标,可实现机械化制种;与传统制种模式相比,混播制种可使父本基本苗减少85%,母本容量增加20%,制种产量增加21.37%,制种综合效益增加31.4%。父母本较好的农艺性状... 相似文献
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Zou Yang Ma Jian-Gang Yue Dong-Mei Zheng Wen-Bin Zhang Xiao-Xuan Zhao Quan Zhu Xing-Quan 《Tropical animal health and production》2017,49(3):653-657
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生物有机肥对烤烟品质及经济效益的影响 总被引：10，自引：0，他引：10
通过田间试验,研究了生物有机肥对烤烟品质、经济效益的影响,结果表明：对烟叶物理特性的影响是适当降低了顶叶的厚度;对烟叶化学成分的影响是下部及下二棚烟叶的糖碱比和氮碱比趋于协调,中上部烟叶糖碱比和氮碱比更加协调;对烟叶品质的影响是提高了致香物的含量,而且致香物总量随着生物有机肥中有机氮比例的增加而增加;对经济效益的影响是各部位烟叶均较对照含量有所增加,中部叶和下部叶以50%生物有机肥处理含量最高,与对照差异显著,其产量和产值均有所增加。 相似文献
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