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内蒙古地区风干牛肉的产品特性及工艺探讨 总被引：2，自引：0，他引：2
在保证了风干牛肉的传统风味与特性的前提下,通过工艺改进,控制风干室的温度和湿度,模拟草原上的风干过程,进行低温与高温2次炸制,采用真空包装与二次灭菌技术,大大缩短了生产周期,提高了生产效率,同时有效地降低了产品中有害及腐败微生物的数量,延长了风干牛肉的货架寿命,使该产品的经济效益明显增加。 相似文献
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为提高实验室保存的乳酸菌N22产抗真菌物质的产量, 以毕赤酵母为指示菌, 产生的抑菌圈大小为评价指标, 通过响应面法优化乳酸菌产抗真菌物质的培养条件。对MRS培养基碳源、氮源进行优化, 考查不同温度、初始pH值和发酵时间对乳酸菌产抗真菌物质的影响, 温度、初始pH值和发酵时间是主要影响因素, 最后采用Box-Behnken试验设计和响应面法分析确定乳酸菌最佳培养条件为34.16 ℃、初始pH值为5.33、培养35.44 h, 此时, 最大抑菌圈直径为16.34 mm, 可取率为97.90%。经验证, 最大抑菌圈直径为16.58 mm。该培养条件增加了乳酸菌抗真菌物质的产量。 相似文献
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Vaccination of chickens with DNA vaccine expressing Eimeria tenella MZ5-7 against coccidiosis 总被引：1，自引：0，他引：1
A chimeric DNA vaccine co-expressing Eimeria tenella MZ5-7 and chicken IL-17 gene was constructed and its efficacy against E. tenella challenge was evaluated. The ORF of MZ5-7 from E. tenella's second generation merozoite and the mature interleukin 17 gene of chicken were cloned into the expression vector of pcDNA4.0 to construct DNA vaccine pcDNA4.0-MZ and pcDNA4.0-MZ-IL17. The expression of aim gene products in vivo was detected by western blotting. The expression of IL-2 and IFN-γ in chicken splenocytes was detected by RT-PCR 7 days post-immunization. The expression levels of the two cytokines in the pcDNA4.0-MZ-IL17 DNA vaccine immunized group were significantly higher than that in the pcDNA4.0-MZ immunized group (p<0.05). Either pcDNA4.0-MZ or pcDNA4.0-MZ-IL17 DNA vaccine could obviously alleviate cecal lesions, body weight loss and increase oocyst decrease ratio. The ACI of pcDNA4.0-MZ-IL17 group was 190, which is higher than that of pcDNA4.0-MZ group and all the three control groups. In short, MZ5-7 was an effective candidate antigen for vaccine and co-expression of cytokine with antigen was an alternative method to enhance the immunity DNA vaccine. 相似文献
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