组胺和5—羟色胺在阿斯匹林对鼠急性炎症反应抗炎效能中的作用     

Sahn.  YP 张光天 《牧业译丛》1993,(4):9-11

  相似文献   

Stepwise in-straw dilution and direct transfer using open pulled straws (OPS) in the mouse: a potential model for field manipulation of vitrified embryos     

Yang QE  Hou YP  Zhou GB  Yang ZQ  Zhu SE 《The Journal of reproduction and development》2007,53(2):211-218

In the present study, mouse blastocysts were employed to investigate the feasibility and efficiency of stepwise in-straw dilution and direct transfer using the open pulled straw (OPS) method. In experiment I, the effects of various vitrification solutions (VS) on embryo survival were examined. After thawing, the expanded blastocyst rates (97.59 and 95.05%) and hatching rates (80.48 and 78.95%) achieved in the EDFS30 [15% ethylene glycol (EG), 15% dimethyl sulfoxide (DMSO), Ficoll, and sucrose] and EFS40 [40% EG, Ficoll, and sucrose] groups were no different from those (96.15% and 83.33%) of the control group. However, the rates in the EFS30 [30% EG, Ficoll, and sucrose] (87.80 and 55.43%) and EDFS40 [20% EG, 20% DMSO, Ficoll, and sucrose] (95.69 and 70.97%) groups were significantly lower than those (96.15 and 83.33%) of the control group (P<0.05). In the experiment II, the effects of the volume of VS in the OPS on the survival of embryos after in-straw thawing were investigated. When the length of the VS in the column was less than 1 cm, the in vitro viability of embryos thawed by stepwise in-straw dilution was no different among the experimental and control groups. The embryos could be successfully thawed by immersing the OPS in 0.5 M sucrose for 3 min and then 0.25 M sucrose for 2 min. In experiment III, the effect of immersion time of the OPS in diluent (PBS) on the viability of vitrified embryos was investigated. After in-straw thawing, OPSs were immersed immediately in 1 ml PBS for 0 to 30 min. When the immersion time of the OPSs in PBS was less than 12 min, in vitro development of the in-straw thawed embryos was no different from that of the controls. In experiment IV, in-straw thawed blastocysts were directly transferred to pseudopregnant mice to examine their in vivo developmental viability. The pregnancy (91.67%) and birth rates (42.42%) of embryos in-straw thawed and directly transferred were no different from those of the unvitrified controls (90.90 and 40%) and embryos thawed by the conventional method (84.61 and 46.94%). These results demonstrate that mouse embryos vitrified with OPS could be successfully thawed by stepwise in-straw dilution and transferred directly to a recipient and that this method might be a model for field manipulation of vitrified embryos in farm animals.  相似文献   

Pax-7 immunoreactivity in the post-natal chicken central nervous system     

Shin DH  Lee KS  Lee E  Chang YP  Kim JW  Choi YS  Kwon BS  Lee HW  Cho SS 《Anatomia, histologia, embryologia》2003,32(6):378-383

In this immunocytochemical study on the constitutive expression of Pax-7 protein in the postnatal chicken brain, Pax-7 showed region and cell type specific expression. In the optic tectum, only cells in grey matter showed positive immunoreactivities (IRs), whereas those in the white matters did not show any IRs. In thalamic nuclei and several pontine nuclei, we also localized Pax-7 positive IRs. On the contrary, in the cerebellum, Pax-7 was mainly localized within the Bergmann glia, whereas Purkinje cells did not show any IRs. In double immunolabelling studies, most of the Pax-7 IRs did not originate from neuroglial cells such as oligodendrocytes, microglia or astrocytes, but from neurons, with the exception of Bergmann glia in the cerebellum. The presence of Pax-7 IRs in the adult chicken brain could suggest that Pax-7 might play a role in maintaining normal physiological function in some postnatal chicken brain cells.  相似文献   

Peste des petits ruminants has been widely present in southern India since, if not before, the late 1980s     

Taylor WP  Diallo A  Gopalakrishna S  Sreeramalu P  Wilsmore AJ  Nanda YP  Libeau G  Rajasekhar M  Mukhopadhyay AK 《Preventive veterinary medicine》2002,52(3-4):305-312

Because previous authorities had suggested that small ruminants were playing a part in the dissemination of rinderpest, and a rinderpest-eradication campaign was about to begin, it was necessary to make precise virus identifications from a number of small-ruminant “rinderpest” outbreaks. When this was done using a database created from passive disease reports, we found that epidemics—reportedly due to rinderpest—were in fact due to peste des petits ruminants (PPRs). Although such cases had been common in India for a number of years, earlier clinical and laboratory reports no longer should be regarded as definitive. PPR outbreaks have been frequent in recent years. Further, we suggest that PPR is not a recent invader of India.  相似文献   

藏雪鸡和家鸡羽毛制剂体外促凝血作用的比较   总被引：4，自引：0，他引：4  

张光天  马森  刘文先  韩秀英 《中兽医医药杂志》1998,17(3):13-14

采用试管法测定了藏雪鸡羽毛制剂和家鸡羽毛制剂对６１只绵羊血液体外促凝血效果。结果生理盐水组凝血时间为４．８４±１．１７（ｍｉｎ）;藏雪鸡羽毛制剂组凝血时间为２．９±１．０３（ｍｉｎ）;家鸡羽毛制剂组为３．３７±０．９４（ｍｉｎ）。表明藏雪鸡羽毛制剂和家鸡羽毛制剂对绵羊血液均有非常显著的体外促凝血作用（Ｐ＜０．０１）,且藏雪鸡羽毛制剂显著优于家鸡羽毛制剂（Ｐ＜０．０５）。  相似文献   

Aluminum-induced decrease in CO2 assimilation in citrus seedlings is unaccompanied by decreased activities of key enzymes involved in CO2 assimilation     

Chen LS  Qi YP  Smith BR  Liu XH 《Tree physiology》2005,25(3):317-324

'Cleopatra' tangerine (Citrus reshni Hort. ex Tanaka) seedlings were irrigated daily for 8 weeks with 1/4 strength Hoagland's nutrient solution containing 0 (control) or 2 mM aluminum (Al). Leaves from Al-treated plants had decreased CO2 assimilation and stomatal conductance, but increased intercellular CO2 concentrations compared with control leaves. On a leaf area basis, 2 mM Al increased activities of key enzymes in the Calvin cycle, including ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), NADP-glyceraldehyde-3-phosphate dehydrogenase (GAPDH), phosphoribulokinase (PRK), stromal fructose-1,6-bisphosphatase (FBPase), and a key enzyme in starch synthesis, ADP-glucose pyrophosphorylase (AGPase), compared with control leaves. Aluminum had no effect on cytosolic FBPase activity, but it decreased sucrose phosphate synthase (SPS) activity. Aluminum had no effect on area-based concentrations of carbohydrates, glucose-6-phosphate (G6P) and fructose 6-phosphate (F6P) or the G6P:F6P ratio, but it decreased the area-based concentration of 3-phosphoglycerate (PGA). Photochemical quenching coefficient (qP) and electron transport rate through PSII were greatly reduced by Al. Non-photochemical quenching coefficient (NPQ) was less affected by Al than qP and electron transport rate through PSII. We conclude that the reduced rate of CO2 assimilation in Al-treated leaves was probably caused by a combination of factors such as reduced electron transport rate through PSII, increased closure of PSII reaction centers and increased photorespiration.  相似文献   

In vitro suppression of quercetin on hypertrophy and extracellular matrix accumulation in rat glomerular mesangial cells cultured by high glucose     

Tang DQ  Wei YQ  Yin XX  Lu Q  Hao HH  Zhai YP  Wang JY  Ren J 《Fitoterapia》2011,82(6):920-926

Quercetin's protective effects on the glomerulosclerosis of diabetic nephropathy (DN) in rat mesangial cells were investigated. The cell cycles, type IV collagen and laminin, TGF-β₁ mRNA, Smad 2/3 and Smad 7, and activities of cell antioxidases were measured. Compared with the high glucose group, quercetin may decrease the cell percentages of G₀/G₁ phase, Smad 2/3 expression, laminin and type IV collagen, and TGF-β₁ mRNA level significantly. The antioxidant capacity, the cell percentages of S phase and Smad 7 expression was significantly increased by quercetin. These results suggest that quercetin is a protective agent against glomerulosclerosis in DN.  相似文献   

Effect of accelerated autolysis of yeast on the composition and foaming properties of sparkling wines elaborated by a champenoise method     

Nunez YP  Carrascosa AV  González R  Polo MC  Martínez-Rodríguez AJ 《Journal of agricultural and food chemistry》2005,53(18):7232-7237

Five mutants (obtained by UV mutagenesis) and the parent strain were selected to produce sparkling wines following the traditional or champenoise method. The wines were aged with the yeast for 9 months, with samples being taken each month for analytical and sensory determinations. The wines elaborated with mutant strain IFI473I demonstrated an accelerated release of protein, amino acids, and polysaccharides. An analysis of the secreted polysaccharides revealed that mannose was the major sugar present. The effects of the products released by yeasts on the foaming properties of the wines were determined by both sensory and instrumental analysis. In all cases, the wines elaborated with mutant strain IFI473I showed improved foaming properties as compared to wines fermented without this strain. Similar results were obtained at a decreased aging time of 6 months, thereby confirming the capacity of IFI473I strain to carry out an accelerated autolysis. These results demonstrate that mutant strain IFI473I can significantly reduce production times of high-quality sparkling wines.  相似文献   

Earthquake prediction: a physical basis   总被引：13，自引：0，他引：13  

Scholz CH  Sykes LR  Aggarwal YP 《Science (New York, N.Y.)》1973,181(4102):803-810

  相似文献   

Asymmetry, its importance to the action and metabolism of abscisic Acid     

Sondheimer E  Galson EC  Chang YP  Walton DC 《Science (New York, N.Y.)》1971,174(4011):829-831

Unlabeled and (14)C-labeled enantiomorphs of abscisic acid (ABA) were obtained through acetylcellulose chromatography and tested as inducers of abscission, as inhibitors of seed germination, and as antagonists of gibberellic acid-induced synthesis and release of alpha-amylase. The activity of the R isomer was either equal to or less than that of the naturally occurring S form. Greatest differences were in the inhibition of root-related growth. In excised beam axes, although uptake of S-[(14C)]ABA is faster, the internal concentration of R-ABA is higher because of faster conversion of S-ABA to inactive metabolic products. In axes a reversal in chirality is less important to the physiological action of ABA than to its metabolism.  相似文献