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Molecular mapping and characterization of traits controlling fiber quality in cotton 总被引：34，自引：1，他引：34
Cotton (Gossypium spp) is the world's leading natural fiber crop. Genetic manipulation continues to play a key role in the improvement of fiber
quality properties. By use of DNA-based molecular markers and a polymorphic mapping population derived from an inter specific
cross between TM-1 (G. hirsutum) and 3-79 (G. barbadense), thirteen quantitative trait loci (QTLs) controlling fiber quality properties were identified in 3-79, an extra long staple
(ELS) cotton. Four QTLs influenced bundle fiber strength, three influenced fiber length, and six influenced fiber fineness.
These QTLs were located on different chromosomes or linkage groups and collectively explained 30% to 60%of the total phenotypic
variance for each fiber quality property in the F2 population. The effects and modes of action for the individual QTLs were characterized with 3-79 alleles in TM-1 genetic
background. The results indicated more recessive than dominant, with much less additive effect in the gene mode. Transgressive
segregation was observed for fiber fineness that could be beneficial to improvement of this trait. Molecular markers linked
to fiber quality QTLs would be most effective in marker-assisted selection (MAS) of these recessive alleles in cotton breeding
programs.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
2.
Choung MG Choi BR An YN Chu YH Cho YS 《Journal of agricultural and food chemistry》2003,51(24):7040-7043
This investigation was conducted to determine the structures and amounts of anthocyanins obtained from seed coats of kidney bean (Phaseolus vulgaris L.) cultivated in Korea. Anthocyanins in the seed coat of kidney bean were extracted with 1% HCl/20% CH(3)OH, and the crude anthocyanin extracts were purified by semipreparative HPLC. Five major anthocyanins were isolated, and their chemical structures were identified by spectroscopic methods (UV-vis, LC/ES-MS, and 1H and 13C NMR). The structures of these five anthocyanins were elucidated as cyanidin 3,5-diglucoside, delphinidin 3-glucoside, cyanidin 3-glucoside, petunidin 3-glucoside, and pelargonidin 3-glucoside. Using RP-HPLC with photodiode array detection, each of the five anthocyanins was separated within 12 min by using a gradient elution. It was proved that the application of RP-HPLC could be an excellent method for determining the composition and contents of anthocyanins in kidney bean. The preponderance of pelargonidin 3-glucoside and delphinidin 3-glucoside are observed in red and black kidney beans, respectively. However, in this study, it is reported for the first time that the contents and composition of anthocyanins in speckled seed depend on the classes of speckle color. The contents of cyanidin 3,5-diglucoside, delphinidin 3-glucoside, cyanidin 3-glucoside, petunidin 3-glucoside, pelargonidin 3-glucoside, and total anthocyanins in seed coats of 16 kidney beans cultivated in Korea were in the ranges of 0-0.04, 0-2.61, 0-0.12, 0-0.17, 0-0.59 and 0-2.78 mg/g of dried seed coats, respectively. 相似文献
3.
Byeong Joo Seo Mi Ran Mun Rejish Kumar V. J Chul-Joong Kim Insun Lee Young-Hyo Chang Yong-Ha Park 《Veterinary research communications》2010,34(4):323-333
Lactic acid producing bacterial strain Probio-16 was isolated from the swine excrements under anaerobic conditions and characterized
by morphology and biochemical characteristics. The strain was further identified by 16S rRNA gene sequencing and phylogeneitc
analysis. The antimicrobial activity of the strain was assayed by testing for growth inhibition of thirteen pathogenic microorganisms.
The strain was tested for antiviral activity against porcine rotavirus in vitro in African green monkey epithelial cell line
TF-104. Antibiotic susceptibility of the strain against 13 antibiotics was tested using disk diffusion method. Phenotypically
and through 16S rRNA gene sequences, Probio-16 was identified and named as Lactobacillus reuteri Probio-16. This strain was resistant to pH 2.0, 5% porcine bile and exhibited antimicrobial activity against all the thirteen
enteric bacterial pathogens tested. Probio-16 supernatant inhibited porcine rotavirus in vitro in TF-104 cell lines. Except
for erythromycin and penicillin G at a concentration of 4 μg/ml, Probio-16 showed resistance to all other thirteen antibiotics
tested. This study indicates L. reuteri Probio-16 as a novel strain with its tolerance to low pH and bile, antimicrobial activity, antibiotic resistance and antiviral
activity against rotavirus, and an ideal probiotic candidate for animal and human application after the proper in vivo experiments. 相似文献
4.
V. J Rejish Kumar Seo Byeong Joo Mun Mi Ran Kim Chul-Joong Lee Insun Kim Hongik Park Yong-Ha 《Tropical animal health and production》2010,42(8):1855-1860
A total of 310 bacterial strains isolated from the porcine gastrointestinal tract were tested for their activity against transmissible
gastroenteritis (TGE) coronavirus and other enteric pathogens. Based on activity, the strains Probio-38 and Probio-37 were
selected as potential probiotics and identified as Lactobacillus plantarum Probio-38 and Lactobacillus salivarius Probio-37 respectively by 16S rRNA gene sequencing. Supernatants of these strains inhibited TGE coronavirus in vitro in ST
cells, without any cytopathic effect even after 72 h of incubation. Both the strains exhibited high survival in synthetic
gastric juice. The strains were resistant to 5% porcine bile and exhibited antimicrobial activity against all the 13 enteric
bacterial pathogens tested. These strains also exhibited resistance to most of the antibiotics analyzed. The inhibition of
transmissible gastroenteritis coronavirus and enteric bacterial pathogens as well as the bile tolerance, high survival in
gastric juice, and the antibiotic resistance indicate that the two isolated bacterial strains are ideal probiotic candidates
for animal application after proper in vivo experiments. 相似文献
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