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Kunio Shirakihara Masahiko Yoshida Machiko Nishino Yoshimi Takao Kouichi Sawada 《Fisheries Science》2001,67(3):430-435
SUMMARY: The vertical distribution of dwarf ayu Plecoglossus altivelis altivelis in the pelagic waters of Lake Biwa was evaluated from June to September in 1995–97 from eight acoustic surveys using a quantitative echosounder. In each survey, echoes from a depth range of 3 m to the sea bottom were collected at a station every 2.7 s for 24 h together with measurements of vertical profiles of water temperature and chlorophyll-a. The ayu's echoes were identified using an underwater video camera. The ayu were observed near the maximum chlorophyll-a depth and above the thermocline. Their density was highest at depths of 4–11 m with 2–4 individuals/m 3 and was almost zero below 20 m. Echo signs were recorded as having a frequent duration of more than 1 h at night, whereas were of a shorter duration in the day. The fish stay in the epilimnion during the day without any clear vertical migration, but are distributed more uniformly at night. The advantages of remaining in the epilimnion are discussed in terms of food availability and predator avoidance. 相似文献
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Kanae SHIOKAWA Chandika D. GAMAGE Nobuo KOIZUMI Yoshihiro SAKODA Kenta SHIMIZU Yoshimi TSUDA Kumiko YOSHIMATSU Jiro ARIKAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(2):221-230
The applicability of the recombinant LipL32 for serodiagnosis of leptospiral infection in field rodents was
assessed in this study. An immunodominant region of LipL32 was determined by monoclonal antibodies, and then,
truncated LipL32 (tLipL32) was designed to contain the region (87–188th amino acid). The tLipL32 was compared
between two recombinant expression hosts Escherichia coli and Pichia
pastoris in ELISA. With field rat sera, tLipL32 expressed by P. pastoris
(tLipL32p) had high antigenicity without background reactions, while tLipL32 expressed by E.
coli (tLipL32e) showed high background reactions, which were reduced by pre-adsorption of sera with
E. coli. To evaluate tLipL32-ELISA, field rat sera were tentatively divided into a
Leptospira infection positive (12 sera) and a negative group (12 sera) based on the results
from flaB gene PCR of kidney samples and WB with whole Leptospira cell.
Consequently, the sensitivity of tLipL32p-ELISA for field rat sera was 83% . A similar result was obtained
from tLipL32e-ELISA with adsorbed sera, (92%). However, sensitivity of tLipL32e-ELISA using sera without an
adsorption treatment was 50%. Regardless of the expression host, tLipL32-ELISA had 100% specificity and
sensitivity in experimentally infected laboratory rats. These results suggest that recombinant LipL32
expressed by P. pastoris is more applicable for serodiagnosis in field rats due to a lack of
background reaction. 相似文献
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Teruo NONOMURA Yoshinori MATSUDA Motoaki TSUDA Kazuto URANAKA Hideyoshi TOYODA 《Journal of General Plant Pathology》2001,67(3):224-227
Commercially available tomato cultivars were hydroponically cultured for inoculation, with Ralstonia solanacearum (K-101), which causes bacterial wilt, by pouring an inoculum suspension into the nutrient solution. Cultivar susceptibility
to the bacteria was evaluated, based on the highest percentage of wilting. Because the length of time for wilt appearance
varied among cultivars, some cultivars appeared to be suppressive to the translocation and/or multiplication of the invading
pathogen. Thus, this hydroponic inoculation system is effective for examining levels of susceptibility in tomato cultivars
to bacterial wilt.
Received 13 December 2000/ Accepted in revised form 27 March 2001 相似文献
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Enose Y Miyake A Ido E Hayami M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(2):283-286
Human immunodeficiency virus (HIV) infects lymphocytes and macrophages via CD4 and chemokine receptors. In this study, the infectivity of a chimeric simian and human immunodeficiency virus (SHIV) having a CCR5-specific HIV-1 envelope gene was examined. A SHIV strain termed SHIV-JRFL could enter cells via CD4 with a chemokine receptor CCR5, not CXCR4, and the viral replication was suppressed by recombinant human RANTES, one of beta-chemokines. The intravenous inoculation of SHIV-JRFL into two rhesus macaques resulted in a systemic infection, though it was rather weak. During the early infection, the production of RANTES from Con A-stimulated PBMCs of the infected monkeys increased. These results suggested that beta-chemokine has the potential to limit the infectivity of an R5-type SHIV. 相似文献
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Sawada Y Yanai T Nakagawa H Tsukamoto Y Tamagawa Y Yokoi S Yanagi M Toya T Sugizaki H Kato Y Shirakura H Watanabe T Yajima Y Kodama S Masui A 《Pest management science》2003,59(1):49-57
Nineteen analogues were synthesized by modifying the tert-butylhydrazine moieties of N'-tert-butyl-N'-(3,5-dimethylbenzoyl)-5-methyl-2,3-dihydro-1,4-benzodioxine-6-carbohydrazide and N'-tert-butyl-N'-(3,5-dimethylbenzoyl)-5-methylchromane-6-carbohydrazide (chromafenozide), and the synthesized analogues were evaluated for their insecticidal activity against Spodoptera litura F. While all of the synthesized analogues had insecticidal activity inferior to those of the lead compounds, several of the analogues nonetheless showed high insecticidal activity. Chromafenozide has shown very high selectivity toward lepidopteran species. 相似文献
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Atsushi IDETA Yoshito AOYAGI Kanami TSUCHIYA Yuuki NAKAMURA Kou HAYAMA Atsushi SHIRASAWA Kenichiro SAKAGUCHI Naomi TOMINAGA Yoshiyuki NISHIMIYA Sakae TSUDA 《The Journal of reproduction and development》2015,61(1):1-6
Embryos obtained via superovulation are necessary for mammalian artificial reproduction, and viability is a key determinant of success. Nonfreezing storage at 4 C is possible, but currently used storage solutions can maintain embryo viability for only 24–48 h. Here we found that 10 mg/ml antifreeze protein (AFP) dissolved in culture medium 199 with 20% (v/v) fetal bovine serum and 25 mM HEPES could keep bovine embryos alive for 10 days at 4 C. We used a recombinant AFP isolated from the notched-fin eelpout (Zoarces elongatus Kner). Photomicroscopy indicated that the AFP–embryo interaction was enhanced at 37 C. Embryos pre-warmed with the AFP solution at 37 C for 60 min maintained high viability, whereas those that were not pre-warmed could live no longer than 7 days. Thus, short-term storage of bovine embryos was achieved by a combination of AFP-containing medium and controlled pre-warming. 相似文献
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