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1.
安普霉素对仔猪内分泌的调控作用及血液生化指示的影响   总被引:4,自引:0,他引:4  
采用单因子试验设计 ,28日龄大长北三元杂交断奶仔猪72头随机分为3组 ,研究饲料中添加不同剂量的安普霉素 (0、20、90mg/kg)对仔猪内分泌的调控作用及血液生化指标的影响。试验期为4周。结果表明 :仔猪日粮中添加90mg/kg的安普霉素可促进机体与生长有关的内分泌活动 ,提高内源激素 (生长激素、胰岛素、甲状腺激素T3)水平 (P<0.05),从而促进肌肉蛋白沉积 ;并具有显著降低血液中氨、尿素氮含量和提高血糖水平的作用 (P<0.05) ,表明安普霉素对仔猪具有增加氮沉积 ,促进蛋白质合成、抑制蛋白质分解的作用  相似文献   
2.
采用3×2×2因子饲养试验和2×2×2因子代谢试验,研究金霉素与赖氨酸和蛋氨酸的交互作用对肉仔鸡生产性能的影响,及金霉素对两种氨基酸代谢的影响。金霉素在饲料中添加150 mg/kg时,对0-3周肉仔鸡具有显著的促生长作用(P<0.01);并显著提高肉仔鸡的饲料采食量和饲料转化率(P<0.01),促进氮沉积(P<0.01)。对4-6周肉仔鸡的生产性能无显著影响(P>0.05);金霉素、赖氨酸和蛋氨酸存在显著的互作关系(P<0.05)。金霉素的促生长效果受饲料中赖氨酸和蛋氨酸含量的影响,当日粮中赖氨酸水平为1.3%,蛋氨酸水平为0.6%时,肉仔鸡的生长性能最高。金霉素对赖氨酸的利用率没有显著影响(P>0.05),对蛋氨酸和胱氨酸的表观利用率具有显著抑制作用(P<0.05)。研究结果表明持续低剂量金霉素与蛋氨酸和赖氨酸具有交互作用,这种互作关系影响彼此对肉仔鸡生产性能的作用效果,金霉素具有提高肉仔鸡赖氨酸需要量的作用。  相似文献   
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SUMMARY: A crossbreeding experiment was carried out in Egypt using a local breed (Baladi Red, BR) and New Zealand White (NZ) to estimate direct heterosis, maternal additive effects and direct sire effects on some growth and carcass traits in rabbits. Data of body weight (at 5, 6, 8, 10, 12 weeks) and daily gains (at intervals of 5-6, 6-8, 8-10, and 10-12 weeks) on 2153 weaned rabbits were collected. Carcass performance at 12 weeks of age (weight and percentages of carcass, giblets, head, fur, blood and viscera) on 213 male rabbits was evaluated. Estimates of coefficients of variation (CV) for most growth and carcass traits were high and ranged from 10.0 to 40.2%. Sire-breed was of considerable importance in the variation of growth traits and some carcass traits, while dam-breed contributed little. Sire-breed × dam-breed interaction affected (P<0.01 or P<0.001) most body weights and gains studied, while it contributed little to the variation of carcass traits. The purebred NZ resulted in rabbits with heavier weights and carcass and with lighter non-edible carcass (blood and viscera) compared to the BR. Heterosis percentages for most growth traits were significant and ranged from 2.5% to 5.0% for body weights and from 0.7% to 9.5% for daily gains. Insignificant positive direct heterosis was observed for most carcass traits. Crossbred rabbits from NZ sires with BR dams were superior to from the reciprocals. Maternal-breed effects on most weights and gains were insignificant, while sire-breed contrasts for some weights and gains proved significant. Postweaning growth and carcass performances of BR-mothered rabbits generally surpassed the NZ mothered, while NZ-sired rabbits were superior at later ages. High edible carcass was observed for BR-sired rabbits, while more non-edible carcass wastes (blood and viscera) for NZ-sired rabbits. Maternal-breed effects appeared to be less important than paternal-breed effects in influencing most weights, gains and carcass traits studied. ZUSAMMENFASSUNG: Heterosis, maternale und direkte Wirkungen bei Wachstums- und Schlachtk?rpermerkmalen in Kaninchenkreuzungen Der Kreuzungsversuch wurde mit lokalen ?gyptischen Rassen (BR) und Neuseeland Wei?en (NZ) zur Sch?tzung direkter Heterosis, maternaler additiver Wirkungen, direkter Vater-Wirkung auf einige Wachstums- und Schlachtk?rpermerkmale von Kaninchen durchgeführt. Angaben über K?rpergewicht (5, 6, 8, 10, 12 Wochen) und Zuwachs (Intervalle 5 bis 6, 6 bis 8, 8 bis 10, 10 bis 12 Wochen) wurden von 2153 abgesetzten Kaninchen gewonnen. Die Schlachtk?rperleistungen bei 12 Wochen Alter (Gewicht und Anteil von Schlachtk?rper, Kopf, Pelz, Blut und Innereien) stammen von 213 m?nnlichen Kaninchen. Sch?tzungen der Variationskoeffizenten (CV) für meiste Wachstums- und Schlachtk?rpermerkmale waren hoch und bewegten sich zwischen 10 und 40,2%. Vaterrasse hatte erheblichen Einflu? auf Unterschiede in Wachstumsrate und einige Schlachtk?rpermerkmale, w?hrend die Mutterrasse weniger beigetragen hat. Interaktion zwischen beiden beeinflu?te die meisten K?rpergewichts- und Zuwacnsleistungen, w?hrend sie wenig zur Variabilit?t der Schlachtk?rpermerkmale beigetragen hat. Reinrassige NZ waren schwerer und hatten weniger nicht nutzbare Schlachtk?rperteile (Blut und Eingeweide) verglichen mit BR. Heterosis-Prozente für die meisten Wachstumsmerkmale waren signifikant und schwankten zwischen 2,5 und 5% für K?rpergewicht, 0,7 bis 9,5% für Zuwachs. Insignifikante positive direkte Heterosis wurde für die meisten Schlachtk?rpermerkmale beobachtet. Kreuzungskaninchen von NZ V?tern waren den reziproken überlegen. Maternale Wirkungen auf meiste Gewichtsmerkmale waren insignifikant, w?hrend Vaterrassenkontraste hierfür signifikant waren. Zuwachs- und Schlachtk?rperleistung von BR ges?ugten Kaninchen haben im allgemeinen die von NZ ges?ugten übertroffen, w?hrend von NZ B?cken gezeugte in sp?teren Altersabschnitten überlegen waren. Hohe Werte für Schlachtk?rper wurden für BR ges?ugte Kaninchen gefunden, w?hrend mehr nicht verzehrbare Abf?lle (Blut und Eingeweide) bei NZ gezeugten vorhanden war. Maternale Rassenwirkungen schienen weniger wichtig als paternale zu sein.  相似文献   
4.
The present study was designed to examine the effects of cell-cycle synchronization protocols, such as confluent, roscovitine treatment and serum starvation, in bovine foetal fibroblasts on synchronization accuracy at G0/G1, viability, apoptosis, necrosis and ploidy for use as a nuclei donor. The cells in 5-10 passages were randomly allocated into three treated groups. Cells were cultured either in Dulbecco's modified Eagle's medium (DMEM) + 10% foetal bovine serum (FBS) until 90% confluent (group 1, confluent), in DMEM + 10% FBS + 30 microM roscovitine for 12 h (group 2, roscovitine), or in DMEM + 0.5% FBS for 5 days (group 3, serum starvation). Most of the cells (>80%) in all groups were arrested at the G0/G1 stage. Although the rates did not differ, cells in group 1 showed an increased cell population arrested at the G0/G1 phase. Significantly (p < 0.05) higher rates of apoptosis occurred in group 3 than in group 1 and 2 (10% vs 6% and 6%, respectively). No differences in chromosomal abnormality were observed among groups. However, by increasing the number of cell culture passages up to 15, significantly (p < 0.05) higher chromosomal abnormality was observed than in 5 and 10 passages (39% vs 28% and 23%, respectively) in group 1. The results clearly indicated that bovine foetal fibroblasts could be effectively synchronized at G0/G1 stages by all the three different treatments, confluent, roscovitine and serum starvation. However, cells in confluent showed reduced apoptosis and necrosis when they underwent 5-10 passages, exhibiting increased percentage of cells with stable chromosome diversity. Hence, cells in confluent merit further studies before they could be used as nuclear donors.  相似文献   
5.
A highly susceptible cowpea,Vigna sinensis cv. Baladi plants were tested as trap plants for eitherMeloidogyne javanica orRotylenchulus reniformis under greenhouse conditions. The plants were gathered by cutting them above the surface of the soil or by uprooting them, 1/2, 1, 3, 6, 12, 24, 36 and 48 days after nematode inoculation. Both of the mentioned nematodes began to mature and lay eggs after the 12th day from their inoculation. Hence, it is advised to pull up cowpea plants from 3–12 days after nematode inoculation. After planting sunflower,Helianthus annus cv. Miak replacing cowpea, the nematode populations were higher, in most cases, on sunflower plants replacing cutting cowpea than those on sunflower replacing uprooted cowpea. The highest percentages of nematode reduction were 98.55 and 99.57 forM. javanica and 95.09 and 92.90% forR. reniformis on sunflower plants replacing cutting and uprooted cowpea plants after 12 days from nematode inoculation, respectively.M. javanica andR. reniformis decreased the length and weight of sunflower plants as affected by planting time and method of cowpea harvest. This method of nematode control is cheaper, easy and pollution free.  相似文献   
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