Effect of cyclodextrin diallyl maleate on methane production, ruminal fermentation and microbes in vitro and in vivo   总被引：1，自引：0，他引：1  

Zeenat Ara LILA  Nazimuddin MOHAMMED  Noriko TATSUOKA  Shuhei KANDA  Yuzo KUROKAWA  Hisao ITABASHI 《Animal Science Journal》2004,75(1):15-22

Effects of β‐cyclodextrin diallyl maleate (CD‐M) on methane production, ruminal fermentation and digestibility were studied both in vitro and in vivo. In in vitro study, diluted ruminal fluid (30 mL) was incubated anaerobically at 38°C for 6 and 24 h with or without CD‐M using hay plus concentrate (1.5:1) as a substrate. The CD‐M was added at different concentrations (0, 1.25, 2.5, 5.0 and 7.5 g/L). The pH of the medium and numbers of protozoa were not affected by the addition of CD‐M. Total volatile fatty acids were increased and ammonia‐N was decreased, molar proportion of acetate was decreased and propionate was increased (P < 0.05) by CD‐M. Methane was inhibited (P < 0.05) by 14–76%. The effect of CD‐M on methane production and ruminal fermentation was further investigated in vivo using four Holstein steers in a cross‐over design. The steers were fed Sudangrass hay and concentrate mixture (1.5:1) with or without CD‐M (2% of feed dry matter) as a supplement. Ruminal proportion of acetate tended to decrease and that of propionate was increased (P < 0.05) 2 h after CD‐M dosing. Total viable counts, cellulolytic, sulfate reducing, acetogenic bacteria and protozoa were unaffected while methanogenic bacteria were decreased (P < 0.05) by CD‐M. The plasma concentration of glucose was increased, whereas that of urea‐N was decreased (P < 0.05). Methane was inhibited (P < 0.05) from 36.4 to 30.1 L/kg dry matter intake by the addition of CD‐M. Apparent digestibilities of dry matter and neutral detergent fiber were not affected while that of crude protein was increased (P < 0.05) in the medicated steers. These data suggested that dietary supplementation of CD‐M decreased methane production and improved nutrient use.  相似文献   

Effect of relationships among clinical mastitis incidence,reproductive performance,and culling rate on the lifetime of dairy cows at Hiroshima University Farm     

Yuzo Kurokawa  Miki Okita  Hirokazu Kubota  Yoshimasa Tsumiyama  Ichiro Chikamatsu  Akiyoshi Tanaka  Taketo Obitsu  Kensuke Kawamura 《Animal Science Journal》2021,92(1):e13591

Farm managers' decision to cull dairy cows is based on the cows' milk production, history of disorder(s), and reproductive performance, each of which affects dairy cows' lifetime (herd life and productive lifespan). We investigated the relationships among the incidence of clinical mastitis (CM), the reproductive performance, and the culling rate. We also assessed the effects of these relationships on the lifetimes of dairy cows, using the records made before and after the introduction of an automatic milking system (AMS) at Hiroshima University Farm. Milk yield, CM incidence density, and culling rate of dairy cows increased after the AMS introduction. The CM incidence was associated with an elongation of the calving interval in cows with the same parity. CM in the 1st parity might have caused the reductions of the cows' lifetime and their parity at culling. A higher age at first calving (AFC) was associated with an increase in culling rate but did not lead to a significant decrease in lifetime. Investigations of the factors mediating CM in the 1st parity or AFC with CM incidence or culling rate in the later stages might contribute to the control of lifetime of dairy cows.  相似文献   

Within-farm variability in number of females mated per week during a one-year period and breeding herd productivity on swine farms     

Koketsu Y  Duangkaew C  Dial GD  Reeves DE 《Journal of the American Veterinary Medical Association》1999,214(4):520-524

OBJECTIVE: To determine the effect of within-farm variability in the number of females mated per week during a 1-year period on annual breeding herd productivity in swine breeding herds and to apply statistical process control charts to measures of within-farm variability. DESIGN: Longitudinal study. SAMPLE POPULATION: 84 swine farms with a female inventory of 390 to 1,491 sows and gilts (mean, 761 females). PROCEDURE: As a measure of within-farm variability in a breeding herd, SD for the number of females mated per week during a 1-year period was evaluated. Two types of production records for 84 farms were evaluated. One file contained within-farm variability (SD) in mated females for each farm, and the second included annual productivity 19 weeks after week of mating. We also defined forewarning limits as mean +/- 2 SD, using a statistical process control chart. RESULTS: Larger within-farm variability in number of mated females was associated with lower annual measurements, such as fewer pigs weaned per mated female per year and lower farrowing rate. In addition, farms that did not have any weeks outside the forewarning limits for number of mated females produced more pigs weaned per mated female per year than those with 1 or more weeks of over- or underproduction. Furthermore, the number of weeks outside of forewarning limits was positively associated with within-farm variability in number of mated females. CLINICAL IMPLICATIONS: We recommend that farm managers determine a target range for the number of females mated per week to prevent large week-to-week variations in breeding herd operations.  相似文献   

Relation of Japanese <Emphasis Type="Italic">Xanthomonas campestris</Emphasis> pv. <Emphasis Type="Italic">vesicatoria</Emphasis> with worldwide strains revealed with three specific monoclonal antibodies     

Kenichi?TsuchiyaEmail author  Christophe C.M.?D’Ursel  Mitsuo?Horita  Yuzo?Nozu 《Journal of General Plant Pathology》2003,69(5):310-315

Strains of Xanthomonas campestris pv. vesicatoria Dye 1978 (Xcv), the causal agent of bacterial spot, have been classified into two groups based on their ability to hydrolyze starch. Three monoclonal antibodies (MAbs), 7AH10, 5HB3, and 4AD2, were produced immunized against the living bacteria and were specific to and could distinguish Xcv strains able or unable to hydrolyze starch (Amy⁺ or Amy^–). The MAb 7AH10, obtained against strain UPB141(Amy^–) reacted in an enzyme-linked immunosorbent assay with all the Amy^– strains (n = 19) and 1 of 11 Amy⁺ strains. Against Xcv 2625, an Amy^– unusual phenotype strain, MAb 5HB3, recognized 97% of our worldwide collection of Xcvs (n = 30). Also against that strain, the MAb 4AD2 reacted with none of the homologous Amy^– phenotypes and with 90% (n = 11) of the heterologous Amy⁺ phenotypes. For all the MAbs, cross reactions with other pathovars or species were less than 4% (n = 67). By assaying a Japanese collection of strains against the three MAbs, the Amy⁺ strains were distinguished from the Amy^– strains, and their relation with other world strains could be demonstrated. All the MAbs reacted with the lipopolysaccharide fraction of the bacterial cell wall during immunoblotting.  相似文献   

Productivity characteristics of high-performing commercial swine breeding farms     

Koketsu Y 《Journal of the American Veterinary Medical Association》2000,216(3):376-379

OBJECTIVE: To determine productivity characteristics of high-performing swine breeding herds in the United States and to determine associations among number of litters per mated female per year (LMFY), number of pigs weaned per sow (PWS), and lactation duration. DESIGN: Cohort study. SAMPLE POPULATION: 1997 productivity records for 685 herds. PROCEDURE: Herds were ranked on the basis of number of pigs weaned per mated female per year, and herds in the upper 10th percentile of this ranking were designated as high-performing herds. Productivity measurements for these herds were compared with values for the remaining herds. RESULTS: High-performing herds had shorter lactation durations and higher mean breeding female inventories than did the remaining herds. High-performing herds also had better reproductive efficiency and used farrowing facilities more efficiently than did the remaining herds. For the high-performing herds, lactation duration was significantly associated with PWS but was not significantly associated with LMFY. In contrast, for the remaining herds, lactation duration was not significantly associated with PWS but was significantly associated with LMFY. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that high-performing commercial swine farms could increase PWS by improving preweaning mortality rate and number of pigs born alive, but that LMFY was already maximal. For other herds, however, shortening lactation duration would likely decrease farrowing interval and improve efficiency of the reproductive cycle without reducing litter size.  相似文献   

Retrospective analysis of trends and production factors associated with sow mortality on swine-breeding farms in USA   总被引：2，自引：0，他引：2  

Koketsu Y 《Preventive veterinary medicine》2000,46(4):249-256

Of the 825 pig farms in USA that mailed in their electronic file containing production records, 604 farms were used to observe breeding-female mortality risk and related factors (herd size, lactation length, parity and season). Multiple regression was used to determine factors associated with annual mortality risk. Analyses of variance were used for comparisons of mortality risks among parity and season groups. Average annual mortality risks during the 1997 period was 5.68%. Average breeding-female inventories and average lactation length on USA farms were 733 and 18.3 days, respectively. Higher annual breeding-female mortality risk was associated with larger herd size, greater parity at farrowing and shorter lactation length (P<0.02). For example, as herd size increases by 500 females, mortality risk increases by 0.44%. Older parity was associated with higher mortality risks. Summer season was also associated with higher mortality risk. Using five-years’ records on 270 farms, annual mortality risk in 1997 was higher than those of 1993 and 1994, while average breeding-female inventory increased and lactation length decreased. It is recommended that producers, especially in large herds, pay more attention to breeding females.  相似文献   

Seasonal changes in digestibility, passage rate and rumen fermentation of alfalfa hay in sika deer (Cervus nippon) under restricted feeding     

Sanae ASANO  Shoshichi IKEDA  Yuzo KUROKAWA  Shuhei KANDA  Hisao ITABASHI 《Animal Science Journal》2007,78(1):28-33

To investigate seasonal variations in the digestive functions of sika deer, five female sika deer were provided with an amount of alfalfa hay cubes equivalent to voluntary food intake during winter. We measured the rate at which the food passed through the digestive tract, digestibility and rumen fermentation during the summer (August), autumn (November), winter (February) and spring (May). Total mean retention time in the digestive tract during summer and autumn was numerically longer than that in winter and spring, but the difference did not reach significance. Organic matter and fiber were less digestible in summer and autumn than in winter and spring (P < 0.05), whereas the digestibility of the dry matter tended to vary with the seasons (P < 0.1). Ruminal pH values seasonally changed (P < 0.01), and were the lowest in autumn. The concentration of ruminal ammonia‐nitrogen differed significantly among the seasons (P < 0.05), increasing in winter and decreasing during spring and summer. The numbers of protozoa changed significantly among the seasons (P < 0.05), being higher in autumn than in winter and spring, and intermediate in summer. The concentration of total volatile fatty acids was not seasonally affected, but the molar percentages of propionic acid and butyric acid significantly changed according to season (P < 0.05 and P < 0.01, respectively), and the ratio of acetic to propionic acid tended to change with the seasons (P < 0.1). The results of this study suggested that the digestive functions in sika deer, fed a commercial diet at a restricted level, differed notably among the seasons and these variations might partially be due to environmental effects.  相似文献   

Climatic factors associated with reproductive performance in English Berkshire pigs and crossbred pigs between Landrace and Large White raised in a subtropical climate region of Japan     

Shiho Usui  Yuzo Koketsu 《Tropical animal health and production》2015,47(8):1547-1552

  相似文献   

Effects of drying and/or quenching histories on lateral tensile deformation properties of water-swollen wood     

Yuka Miyoshi  Tomoaki Kiryu  Keisuke Kojiro  Yuzo Furuta 《Journal of Wood Science》2015,61(5):525-528

  相似文献   

Dog Age and Breeds Associated with High Plasma Cholesterol and Triglyceride Concentrations     

Shiho USUI  Yasushi MIZOGUCHI  Hidemi YASUDA  Nobuaki ARAI  Yuzo KOKETSU 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(2):269-272

  相似文献