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To facilitate blood collection from blood donor dogs, arteriovenous fistulas were established between the common carotid arteries and external jugular veins in five adult dogs. Twelve to 16 mm, side-to-side anastomoses were created using simple interrupted 6–0 polypropylene sutures. Starting 1 month after the surgical procedure, 500 ml of blood was collected from each dog as required. Electrocardiography, thoracic radiography, and cardiac output and rate were used to monitor cardiac changes. Blood flow characteristics, and proliferative and dystrophic cellular changes occurring in the vessels were documented in one dog.
The mean blood collection times were 2 minutes and 45 seconds from the fistula site and 9.0 minutes from the opposite jugular vein. Ventricular hypertrophy and myocardial changes were observed on electrocardiogram in two dogs, and radiographic evidence of pulmonary hypertension was noted in three dogs. Reversed blood flow was documented in the common carotid artery and external jugular vein distal to the arteriovenous fistula. Four dogs were still in use as blood donors 1 to 1 1/2 years after establishment of the fistulas. Clinical signs of congestive heart failure were not observed.  相似文献   
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An approach combining ventral midline celiotomy with transdiaphragmatic thoracotomy was evaluated in eight healthy cats for ligation of the thoracic duct system. Evans Blue solution was injected into the right colic lymph node to outline the intestinal lymphatic trunk and the thoracic duct system. Three cats (group 1) had mesenteric lymphangiograms and three (group 2) had only lymph node dye injection before thoracic duct ligation. The thoracic duct system was ligated with hemostatic clips just cranial to the aortic hiatus of the diaphragm, through a left transdiaphragmatic thoracotomy. Two cats (group 3) had prethoracotomy mesenteric lymphangiograms and thoracic duct isolation without ligation. Mesenteric lymphangiography was performed immediately after the surgery. In all of the cats, an absence of contrast medium in the thoracic duct system cranial to the surgical site was interpreted as complete obstruction. Four weeks after ligation, there was complete obstruction of the thoracic duct system with alternate lymphaticovenous communications in four of the six cats with ligated thoracic duct systems. Partial obstruction of the thoracic duct system with alternate lymphaticovenous communications was present in the other two cats. Both cats without thoracic duct ligation had patent thoracic duct systems. At necropsy of the six cats with ligated thoracic ducts, there was mild focal lymphadenitis of injected lymph nodes in three cats. The wall of the aorta adjacent to the hemostatic clips was normal in all six cats. The surgical technique was simple and provided excellent exposure. Vital staining with Evans Blue helped visualize the thoracic duct system, but mesenteric lymphangiography did not. Postligation lymphangiography was not of value in identifying incomplete ligation.  相似文献   
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