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长白山区野生地椒百里香嫩枝扦插繁殖研究   总被引:1,自引:0,他引:1  
以长白山区野生地椒百里香为试材,研究了不同浓度、不同生长调节剂、不同时间处理对地椒百里香嫩枝扦插繁殖的影响。结果表明:植物生长调节剂对平均生根率的影响为ABTNAAIBA,分别为94.22%、90.69%、81.33%。但是嫩枝扦插后根部长势最好在NAA1 000mg/L(1s)。因此,长白山区野生地椒百里香嫩枝扦插时,建议选择ABT生根剂。选用ABT生根剂时,处理浓度间差异不大,但浓度500mg/L(2min)时优于其它处理组。当选用NAA溶液时,处理浓度间差异较大,且随着处理浓度的增加效果更佳,最佳处理为1 000mg/L(1s)。当选用IBA溶液时,随着浓度增加,生根率及根部长势越差,50mg/L(12h)为最佳处理。  相似文献   
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用乙醇超声提取法提取茅苍术根茎物质,并进行杀蚊有效活性成分分离,采用幼虫浸渍法测定其对敏感型淡色库蚊、野生型地下家蚊和白纹伊蚊3龄幼虫的活性。结果发现:乙醇粗提物对淡色库蚊、地下家蚊和白纹伊蚊的24h LC_(50)值分别为22.71、36.30、23.51μg/mL。不同溶剂萃取物对白纹伊蚊生物活性测定结果表明,活性成分主要在正己烷相中,其LC_(50)为16.87μg/mL;而乙酸乙酯相和水相成分基本无活性。进一步对正己烷萃取物以硅胶柱层析分离的不同成分进行生物活性测定,活性成分主要为H2和H6;白纹伊蚊3龄幼虫在其25μg/mL质量浓度下24h死亡率均达100%。结果表明茅苍术的杀虫活性成分对敏感型淡色库蚊、野生型地下家蚊和白纹伊蚊的幼虫均具有较强生物活性,具有新型杀蚊剂开发潜力。  相似文献   
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Attempts to increase production and improve farm environments have been made for several years. Rumen motility (RM) is one of the biological parameters that provides essential information of individuals in ruminants, and it is usually evaluated by auscultation. The study was aimed to examine RM using the 3-axis accelerometer (3XA) in cattle. The manufactured 3XA were placed in the reticulum (3XA-R) and implanted in the subcutaneous layer of the brisket (3XA-SC), respectively, and the accelerations were compared following intramuscular injection of xylazine (0.05 mg/kg) or saline in experiment 1 and of xylazine (0.05 mg/kg) or atropine (0.04 mg/kg) in experiment 2. In experiment 3, the dose-dependent decrease of RM was evaluated following xylazine administration (0, 0.05, 0.1 mg/kg) in the 3XA-R equipped cows via a 3 × 3 Latin square method. In experiment 1, saline-treated animals showed a continuous fluctuation while the frequency and amplitude of 3XA-R in xylazine-injected cows were reduced after administration. The acceleration of 3XA-SC was changed after administration, but not abruptly. Among the motion parameters, V2 was calculated only using X- and Z-axis acceleration in consideration of the cylindrical shape, and it showed the apparent difference between pre- and post-xylazine administration. In experiment 2, the V2 of 3XA-R was decreased after atropine administration while that of 3XA-SC was maintained. In experiment 3, a dose-dependent V2 decrement of 3XA-R after xylazine administration was observed and lasted for 40 and 80 min in doses of 0.05 mg/kg and 0.1 mg/kg, respectively. In conclusion, The 3XA detected the decrease in RM efficiently and processed the data wirelessly without interference from body movement. This technology will help detect problems early and prevent a decline in cattle productivity.  相似文献   
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Mesenchymal stem cells (MSCs) possess regenerative and immunomodulatory properties and can control the immune dysregulation that leads to β-cell destruction. Stem-cell transplantation could thus manage insulin-dependent diabetes mellitus (IDDM) in dogs. In this pilot study, we aimed to assess canine adipose tissue-derived MSCs (cAT-MSCs) transplantation as a treatment for canine diabetes mellitus. This study included four dogs with over a year of insulin treatment for IDDM, following diagnosis at the Veterinary Medicine Teaching Hospital of Seoul National University. Allogenic cAT-MSCs were infused intravenously three or five times monthly to dogs with IDDM. Blood and urine samples were obtained monthly. General clinical symptoms, including changes in body weight, vitality, appetite, and water intake were assessed. Three of the four owners observed improvement of vitality after stem cell treatment. Two of the four dogs showed improvement in appetite and body weight, polyuria, and polydipsia. C-peptide has increased by about 5–15% in three of the cases, and fructosamine and HbA1c levels have improved in two of the cases. Hyperlipidemia was resolved in two of the dogs, and there was no concurrent bacterial cystitis in any of the dogs. C-peptide secretion and lipid metabolism are associated with diabetic complications. Improvement in these parameters following the treatment suggests that cAT-MSC transplantation in dogs with IDDM might help to improve their insulin secretory capacity and prevent diabetic complications.  相似文献   
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The paper examines some of the physical and chemical properties of a deep red friable clay profile (Nitosol) developed in the Kenya highlands over trachyte. This soil is widely planted to coffee. The profile is remarkable for the depth and uniformity of the B horizon and for its stable microaggregation. Despite a clay content of 50–60% throughout the deep solum, the soil feels and handles like a loam. Work with radioactive phosphorus on samples from the profile and from nearby experimental plots under coffee showed that the activity, and the amounts, of surface phosphate fractions decreased in the order Fe-P, Al-P and Ca-P, but the order of decrease of specific surface activity was Al-P, Fe-P, Ca-P. The relative availabilities of these forms is discussed.  相似文献   
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The herbicidal action of clove oil on cucumber seedlings was characterized under light and dark conditions. Paraquat showed herbicidal activity only under the light condition, whereas the clove oil displayed herbicidal activity in both the light and the dark condition. Specifically, wilting and water content reduction progressed rapidly under both the light and the dark condition 1 h after the clove oil treatment, whereas the paraquat damage occurred only under the light condition 5 h after treatment. The malondialdehyde concentration increased more with the clove oil treatment than with the paraquat treatment under the light and dark conditions. The superoxide dismutase (SOD) activity was stimulated, but the catalase activity decreased in the clove oil treatment. In contrast, both the SOD and catalase activity decreased in the paraquat treatment. These results suggest that clove oil exerts herbicidal effects through a mechanism that is different from that of paraquat.  相似文献   
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