Introduction   总被引：1，自引：0，他引：1  

Pedroli  Bas  Harms  Bert 《Landscape Ecology》2002,17(1):1-12

The realization of strategies for sustainable land use assumes specificresearch concepts from the local to the global scale (micro-, meso- andmacroscale). Therefore, landscape ecological science has to provideinvestigation methods for all these different scales. By combiningtop-down and bottom-up approaches in addition tocoupled GIS-model applications and traditional methods, the investigation oflandscape ecological structures and processes seems to be possible. Thepresented studies show this approach on examples of two study areas in EasternGermany: A watershed of 400 km² and an administrativedistrict of about 4000 km². The scale-specificapplicability of several models and methods were tested for theseinvestigations, and the validation of the calculated results are presented. Animportant outcome of the project should be the prevention of conflicts betweenagriculture, water management and soil, and water and nature conservation;based onrecommendations for land use variants with decreased pollutant loading withinagricultural areas. The scale specific investigations can be considered as abase for establishing sustainable land use.This revised version was published online in May 2005 with corrections to the Cover Date.  相似文献   

Landscape – What’s in it? European Landscape Research at a Turning Point     

Bas Pedroli  Teresa Pinto-Correia 《Landscape Ecology》2006,21(3):313-313

  相似文献   

Functional region identification in proteins by accumulative-quantitative peptide mapping using RP-HPLC-MS     

Kuipers BJ  Bakx EJ  Gruppen H 《Journal of agricultural and food chemistry》2007,55(23):9337-9344

A new method was developed to identify regions in proteins from which peptides are derived with specific functional properties. This method is applicable for systems in which peptides of a hydrolyzed protein possess specific functional properties, but are too large to be sequenced directly and/or the peptide mixture is too complex to purify and characterize each peptide individually. In the present work, aggregating peptides obtained by proteolytic hydrolysis of soy glycinin were used as a case study. The aggregating peptides are isolated and subsequently further degraded with trypsin to result in peptides with a mass <5000 Da to enable sequence identification using RP-HPLC-MS in combination with MS/MS. Prior to RP-HPLC the peptides are fractionated using anion and cation exchange chromatography. The fractions obtained are analyzed with RP-HPLC-MS. The peptides, with identified sequences, were quantified using the peak areas of the RP-HPLC chromatograms measured at 214 nm. Next, the peak areas were corrected for the molar extinction coefficient of the individual peptides, followed by accumulative-quantitative peptide mapping. The results show that in complex systems, based on the method described, the regions in the parental protein from which the functional peptides originate can be properly identified.  相似文献   

Bioeconomy – an emerging meta-discourse affecting forest discourses?     

Helga Pülzl  Daniela Kleinschmit  Bas Arts 《Scandinavian Journal of Forest Research》2014,29(4):386-393

The term bioeconomy and closely related notions like bio-based economy or knowledge-based bioeconomy (KBBE) are increasingly used by scientists and politicians in the last years. It does therefore have the potential of becoming an influential global discourse. Its role is however so far unclear. The general assumption that guides this paper is that discourses, resulting ideas and arguments are generally said to have performative power. They shape actors' views, influence their behaviour, impact on their beliefs and interests and can cause institutional change in a given society. Thus, the aim of this paper is twofold: first, it aims to analyse whether the ideas used in a bioeconomy discourse differs from those in other global meta-discourses of the last decades affecting forest discourses, such as the ecological modernization discourse or the sustainable development discourse. Second, this paper aims to analyse whether and how the bioeconomy discourse has started (or not) to reshape or overshadow the “classical” forest discourses, such as sustainable forest management, forest biodiversity or forest and climate change.  相似文献   

Pharmacokinetics of enrofloxacin after intravenous and intramuscular administration in Angora goats.   总被引：1，自引：0，他引：1  

M Elmas  B Tras  S Kaya  A L Bas  E Yazar    E Yarsan 《Canadian journal of veterinary research》2001,65(1):64-67

Pharmacokinetics and bioavailability of enrofloxacin were determined after single intravenous (IV) and intramuscular (IM) administrations of 5 mg/kg body weight (BW) to 5 healthy adult Angora goats. Plasma enrofloxacin concentrations were measured by high performance liquid chromatography. Pharmacokinetics were best described by a 2-compartment open model. The elimination half-life and volume of distribution after IV and IM administrations were similar (t1/2beta, 4.0 to 4.7 h and Vd(ss),1.2 to 1.5 L/kg, respectively). Enrofloxacin was rapidly (t1/2a, 0.25 h) and almost completely absorbed (F, 90%) after IM administration. Mean plasma concentrations of enrofloxacin at 24 h after IV and IM administration (0.07 and 0.09 microg/mL, respectively) were higher than the minimal inhibitory concentration (MIC) values for most pathogens. In conclusion, once-daily IV and IM administration of enrofloxacin (5 mg/kg BW) in Angora goats may be useful in treatment of infectious diseases caused by sensitive pathogens.  相似文献   

Influence of trenbolone acetate combined with estradiol-17 beta on growth performance, body characteristics, and chemical composition of goat kids fed milk and slaughtered at different ages.     

P Schmidely  P Bas  A Rouzeau  J Hervieu  P Morand-Fehr 《Journal of animal science》1992,70(11):3381-3390

The effects of anabolic agents (5 mg of estradiol-17 beta + 30 mg of trenbolone acetate) on body characteristics and chemical composition of gain were studied in 58 intact male goat kids fed milk replacer. Four kids were slaughtered at 7 d of age to constitute the initial group. The other kids were allotted in a 2 x 3 factorial arrangement with slaughter age (41, 49, and 56 d of age) and treatment (control and implanted at 21 d old) as factors. Energy intake decreased during the first 4 wk after treatment; implanted kids had the same ADG as controls but a better energy efficiency (P < .05). During the last week of the trial, energy intake was the same; treated kids tended (P < .10) to have a higher empty body gain (EBG). Anabolic agents increased carcass (P < .10) and hide proportions (P < .01) in empty BW and in EBG for all groups (except for carcass at 56 d old). Anabolic agents reduced the contribution of adipose tissues (P < .05), empty digestive tract (P < .05), and other organs (P < .01) to EBG. At all slaughter ages, the chemical composition of carcass and hide wet tissues, all dissectible adipose tissues, and EBG were altered by treatment. In these tissues (except for mesenteric fat tissue), water content increased and lipid content decreased (P < .05), but these effects diminished with age. Expressed on a DM basis, the CP content of the treated carcasses was increased in all groups (P < .05). Implanting high doses of steroids altered nutrient partitioning, which reduced fat and increased water and protein in the body.  相似文献   

Identification of strong aggregating regions in soy glycinin upon enzymatic hydrolysis     

Kuipers BJ  Gruppen H 《Journal of agricultural and food chemistry》2008,56(10):3818-3827

Upon hydrolysis with chymotrypsin, soy glycinin has a strong tendency to aggregate. The regions of glycinin from which the aggregating peptides originate were identified by accumulative-quantitative peptide mapping. To this end, the aggregating peptides were further hydrolyzed with trypsin to obtain peptides of which the sequence can be identified using RP-HPLC-MS/MS. This resulted in a hydrolysate in which 90% of the proteinaceous material was dissolved. The soluble fraction was analyzed using the method of accumulative-quantitative peptide mapping: fractionation using ion exchange chromatography, followed by identification of peptides by RP-HPLC-MS/MS, quantification based on the absorbance at 214 nm, and finally peptide mapping. For the peptide mapping the proportions in which each of the five glycinin subunits are present, as determined by Edman degradation, were taken into account. The results showed that mainly the basic polypeptide and a part of the acidic polypeptide, close to the location of the disulfide bridge between the basic and acidic polypeptides, are present in the aggregating peptide fraction. On the basis of the results obtained, an aggregation mechanism was proposed. The hydrophilic acidic polypeptides shield the hydrophobic basic polypeptides, and the former are preferentially degraded upon hydrolysis. This results in a net increase in hydrophobicity of the remaining material, which mainly consists of the basic polypeptide fragments. This increase in hydrophobicity is proposed to be the driving force in the aggregation of chymotrypsin-derived peptides of glycinin.  相似文献   

Cross-sectional study on acrylamide hemoglobin adducts in subpopulations from the European Prospective Investigation into Cancer and Nutrition (EPIC) Study     

Vesper HW  Slimani N  Hallmans G  Tjønneland A  Agudo A  Benetou V  Bingham S  Boeing H  Boutron-Ruault MC  Bueno-de-Mesquita HB  Chirlaque D  Clavel-Chapelon F  Crowe F  Drogan D  Ferrari P  Johansson I  Kaaks R  Linseisen J  Lund E  Manjer J  Mattiello A  Palli D  Peeters PH  Rinaldi S  Skeie G  Trichopoulou A  Vineis P  Wirfält E  Overvad K  Strömberg U 《Journal of agricultural and food chemistry》2008,56(15):6046-6053

Acrylamide exposure was investigated in subgroups of the EPIC study population (510 subjects from 9 European countries, randomly selected and stratified by age, gender, and smoking status) using hemoglobin adducts of acrylamide (HbAA) and its primary metabolite glycidamide (HbGA). Blood samples were analyzed for HbAA and HbGA by HPLC/MS/MS. Statistical models for HbAA and HbGA were developed including body mass index (BMI), educational level, and physical activity. A large variability in acrylamide exposure and metabolism between individuals and country groups was observed with HbAA and HbGA values ranging between 15-623 and 8-377 pmol/g of Hb, respectively. Both adducts differed significantly by country, sex, and smoking status. HbGA values were significantly lower in high alcohol consumers than in moderate consumers. With increasing BMI, HbGA in nonsmokers and HbAA in smokers decreased significantly. In the assessment of potential health effects related to acrylamide exposure, country of origin, BMI, alcohol consumption, sex, and smoking status should be considered.  相似文献   

Fine-earth translocation by tillage in stony soils in the Guadalentin, south-east Spain: an investigation using caesium-134     

Timothy A. Quine  Gerard Govers  Jean Poesen  Des Walling  Bas van Wesemael  Jose Martinez-Fernandez 《Soil & Tillage Research》1999,51(3-4):279-301

Tillage erosion is increasingly recognised as an important soil erosion process on agricultural land. In view of its potential significance, there is a clear need to broaden the experimental database for the magnitude of tillage erosion to include a range of tillage implements and agricultural environments. The study discussed in this paper sought to address the need for such data by examining tillage erosion by a duckfoot chisel plough in stony soils on steep slopes in a semi-arid environment. Results of the investigation of coarse fraction (rock fragment) translocation by tillage in this environment have been presented elsewhere and the paper focuses on tillage translocation and erosion of the fine earth. Tillage translocation was measured at 10 sites, representing both upslope and downslope tillage by a duckfoot chisel plough on five different slopes, with tangents ranging from 0.02 to 0.41. A fine-earth tracer, comprising fine earth labelled with ¹³⁴Cs, was introduced into the plough layer before tillage. After a single pass of the plough, incremental samples of plough soil were excavated and sieved to separate the fine earth from the rock fragments. Translocation of the fine-earth tracer was established by analysing the ¹³⁴Cs content of the samples of fine earth. These data were used to establish translocation distances for each combination of slope and tillage direction. Translocation distances of the fine earth were not significantly different from translocation distances of the coarse fraction. For all sites, except uphill on the 0.41 slope, translocation distances were found to be linearly related to slope tangent. The soil flux due to tillage for each site was calculated using the translocation distance and the mass per unit area of the plough layer. For slopes with tangents <0.25, the relationship between soil flux and tangent was linear and the soil flux coefficient derived was 520–660 kg m⁻¹ per pass. This is much larger than the coefficients found in other studies and this high magnitude is attributed to the non-cohesive nature and high rock fragment content of the soil in this investigation. A second contrast with previous studies was found in non-linearity in the relationship between soil flux and tangent when steeper slopes were included. This was a product of variation in plough depth between the steepest slopes and the remainder of the study area. On the basis of the study it is suggested that an improved understanding of tillage erosion may be obtained by considering the dual processes of tillage detachment (mass per unit area of soil subject to tillage) and tillage displacement (equivalent to translocation distance per pass) in assessing, comparing and modelling tillage translocation. An improved model is proposed that recognises the complexity of soil redistribution by tillage, provides a framework for process-based investigation of the controls on tillage fluxes, and allows identification of potential self-limiting conditions for tillage erosion.  相似文献   

Detection of <Emphasis Type="Italic">Xanthomonas axonopodis</Emphasis> pv. <Emphasis Type="Italic">manihotis</Emphasis>, the causal agent of cassava bacterial blight diseases in cassava (<Emphasis Type="Italic">Manihot esculenta</Emphasis>) in Ghana by polymerase chain reaction     

Muntala Abdulai  Hüseyin Basım  Esin Basım  Derya Baki  Nurhan Öztürk 《European journal of plant pathology / European Foundation for Plant Pathology》2018,150(2):471-484

Xanthomonas axonopodis pv. manihotis (Xam) is the causal agent of cassava bacterial blight (CBB) disease. CBB is a major constraint to cassava cultivation in Ghana. In this study, a survey was conducted in eight regions of Ghana to assess the presence of CBB disease. Out of the eight regions visited, CBB, though at different prevalence, was observed in five regions. Cassava plants samples showing suspected bacterial blight symptoms were collected for analysis by Polymerase Chain Reaction (PCR). The results of the analysis showed that Ashanti region had the highest prevalence in percentage of CBB, which recorded (70%), followed by Volta region (60%); Brong Ahafo region (40%); Eastern region (40%) and Greater Accra region (20%). Morphological examination of the putative pathogen was carried out on Cefazolin trehalose agar (CTA) and Nutrient agar (NA) media. The isolates were subjected to conventional PCR using Xanthomonas genus specific primer, RST2/RST3, Xam specific Variable Number Tandem Repeat (VNTRs) loci, XaG1_67F/R and X-gumD primers, which produced 840, 446 and 402 bp, respectively. The isolates also tested positive with SYBR Green fluorescent dye, using Real-time PCR. The resulting PCR products were sequenced and analyzed using a BLASTn program, which revealed homology between 93 and 100% with several other Xam strains retrieved from GenBank nucleotide database. The pathogenicity test of the isolates on the susceptible Esam cassava variety produced symptoms typical of Xam and the pathogen was consistently re-isolated from the inoculated cassava plants and thereby satisfying the Koch’s postulates.  相似文献