Use of in vitro assays to identify antibiotics that are cytotoxic to normal equine chondrocytes and synovial cells     

Lynn Pezzanite  Lyndah Chow  Gabriella Piquini  Gregg Griffenhagen  Dominique Ramirez  Steven Dow  Laurie Goodrich 《Equine veterinary journal》2021,53(3):579-589

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Growth and reproduction of the lion's paw scallop Nodipecten subnodosus in a suspended culture system at Guerrero Negro lagoon,Baja California Sur,Mexico     

Marcial Arellano‐Martínez  Bertha Patricia Ceballos‐Vázquez  César Ruíz‐Verdugo  Edgar Pérez de León  Rafael Cervantes‐Duarte  Pedro Martin Domínguez‐Valdéz 《Aquaculture Research》2011,42(4):571-582

The lion's paw scallop, Nodipecten subnodosus (Sowerby) has considerable aquacultural potential due to its fast growth and large adductor muscle. Prior investigations throughout northwestern Mexico's littoral have reported highly variable growth rates; furthermore, no studies are available of the environment on growth and gametogenesis in this species under culture conditions. This investigation assesses the effect of food availability and temperature on the growth and gametogenesis of N. subnodosus in a suspended culture system at Guerrero Negro lagoon, Mexico. After 1 year of cultivation, N. subnodosus reached 69.13 mm in shell height (SH) (0.196 mm day^?1, 14 months old). Two significant growth spurts were observed: over the two first months of culture (August and September 2001, mean growth rate 0.4 mm day^?1) and in September 2002 (0.3 mm day^?1), both related to high temperatures and chlorophyll a concentrations. The onset of gametogenesis occurred in April 2002, with an increase in temperature (10‐month‐old scallops, 54.5 mm SH). The first spawning occurred in October and November (86.2 and 93 mm SH), with peak temperatures. These results, together with the analysis of previous reports, indicate that N. subnodosus has a higher preference for temperate areas; therefore, the Guerrero Negro lagoon appears to be a suitable site to culture this species.  相似文献   

Cryopreservation effects on the sperm quality of cachama blanca Piaractus brachypomus (Cuvier 1818)     

Juan Antonio Ramirez‐Merlano  Víctor Mauricio Medina‐Robles  Pablo Emilio Cruz‐Casallas 《Aquaculture Research》2011,42(6):738-745

The effects of straws volume, cryoprotectants and thawing temperatures were evaluated on the sperm quality of cachama blanca Piaractus brachypomus (Cuvier), an important Colombian fish species. Sexually mature fish were induced to ovulation or spermiation with a carp pituitary extract. A pool of suitable sperm samples was diluted in glucose, egg yolk, dimethyl sulphoxide (DMSO‐10%), methanol (MET‐10%) or ethylene glycol (ETG‐5%) and packed in 0.5, 2.5 or 5.0 mL straws and frozen in nitrogen vapour. The thawing process was performed in a 35 or an 80 °C water bath. The fertility was evaluated after 6 h post fertilization. The highest motility percentage (33 ± 3%) was observed with sperm cryopreserved with DMSO, packed in 5 mL straws and thawed at 35 °C. The treatments with DMSO and MET packed in 0.5 and 5.0 mL straws and thawed at 35 °C showed the highest fertility (higher than 71%) and the lowest fertility was obtained with MET‐2.5 mL (9 ± 5%). In all the treatments, a significant decrease in the sperm quality was observed at 80 °C. Sperm cryopreserved with DMSO‐10% or MET‐10%, packed in 2.5 or 5.0 mL straws are suitable to achieve acceptable fertilization and to fertilize high amounts of eggs.  相似文献   

Synovial fluid from a kid with polyarthritis     

Alleman AR  Henson KL  Polkes A  Ramirez S  Brown M 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1999,28(2):64-66

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Bovine TB: studies on the risk of herd breakdown     

Medley G  Green L  Ramirez A 《The Veterinary record》2003,152(2):59-60

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Developmental Consequences of Sexual Dimorphism During Pre-implantation Embryonic Development     

A Gutiérrez-Adán  M Perez-Crespo  R Fernandez-Gonzalez  MA Ramirez  P Moreira  B Pintado  P Lonergan  D Rizos 《Reproduction in domestic animals》2006,41(S2):54-62

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Hyperthyroidism Associated with a Thyroid Adenocarcinoma in a 21-Year-Old Gelding     

Sammy Ramirez  Jill J. McClure  Rustin M. Moore  Karen J. Wolfsheimer  Stephen D. Gaunt  Mustajab H. Mirza  Wayne Taylor 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1998,12(6):475-477

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Empirical models to predict LAI and aboveground biomass of Coffea arabica under full sun and shaded plantation: a case study of South of Minas Gerais,Brazil     

Priscila Pereira Coltri  Jurandir Zullo Junior  Vincent Dubreuil  Glaucia Miranda Ramirez  Hilton Silveira Pinto  Gustavo Coral  Camila Giorgi Lazarim 《Agroforestry Systems》2015,89(4):621-636

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Stocking density effects on larval growth and survival of two catarina scallop, Argopecten ventricosus (= circularis) (Sowerby II, 1842), populations     

A M Ibarra  J L Ramirez  G A Garcia 《Aquaculture Research》1997,28(6):443-451

Two populations of catarina scallop, Argopecten ventricosus (= circularis) (Sowerby II, 1842), were evaluated for their larval growth and survival responses to different stocking densities. Larvae from the Magdalena population had a significantly higher growth but equal survival at a density of 10 larvae ml^?1 compared with 20 larvae ml^?1. Larvae from the Concepcion population grew better at densities of 15 and 20 larvae ml^?1 than at densities of 5 and 10 larvae ml^?1, although survival was lower at higher densities. The differing response of these two populations to increased stocking densities is explained as a possible consequence of different population adaptations related to behavioural and physiological mechanisms. The importance of establishing optimum stocking densities for molluscan larvae is stressed in two contexts: maximizing growth and survival for different populations when artificially reared, and understanding the effects of stocking densities on phenotypic variance, especially during the course of genetic studies.  相似文献   

Sperm Quality Over Consecutive Spermatophore Regenerations in the Pacific White Shrimp Litopenaeus vannamei   总被引：1，自引：0，他引：1  

Bertha P.  Ceballos-Vázquez Benjamin  Aparicio-Simón Elena  Palacios Ilie S.  Racotta 《Journal of the World Aquaculture Society》2004,35(2):178-188

The reproductive potential of males plays an important role in the productivity of captive penaeids. Males present a large variability in their reproductive potential, and thus the selection of appropriate males for reproduction is highly desirable. The present study compares sperm quality at the beginning of the experiment (baseline values) with variations in sperm quality as a result of consecutive spermatophore regenerations. This was done to evaluate possible predictive criteria for optimal sperm quality based on morphological and biochemical criteria in pond-reared Pacific white shrimp Litopenaeus vannamei males. Sperm quality was similar between left and right spermatophore. Sperm count and proportion of normal sperm increased by 86 and 65% (P < 0.05) respectively, from baseline values to third spermatophore regeneration. Proportion of dead sperm progressively decreased with consecutive regenerations, attaining values 33% lower by the third regeneration, compared to baseline values. This indicates that no decrease in sperm quality occurs in L. vannamei during consecutive regenerations and that the existing spermatophores at stocking of males should be expelled to have higher sperm quality in regenerated spermatophores. Furthermore, this procedure allows selecting individuals with high baseline sperm quality based on significant correlations between baseline and regenerated sperm quality observed in the present work. Baseline values of glucose concentration in the hemolymph were positively correlated to baseline values of sperm count (r = 03, P < 0.05). In contrast, baseline values of several lipids in the hemolymph were negatively correlated to several traits of sperm quality at first sampling (baseline values) or at first regeneration. These results are discussed in terms of nutritional requirements of males and of possible predictive criteria of sperm quality.  相似文献