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In 2003, a steer carcass was condemned at a Central Queensland abattoir because of metastatic tumors. In addition, a granulomatous lesion was found in the mediastinal lymph node. Histological examination showed this to be a pyogranuloma, typically associated with Rhodococcus or the Nocardia/Streptomyces group. However, in this case, the only etiological agent was an acid-fast bacillus, which would normally be associated with a more fibrous lesion. A number of nucleic acid-based techniques were used, and the isolate was identified as Mycobacterium asiaticum. This organism is a rarely encountered opportunistic pathogen of humans, associated with subtropical climates. This is the first report of this organism causing infection in cattle. The similarities between this case and cases of human disease are discussed.  相似文献   
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Objective-To evaluate adverse effects and survival times in dogs with osteosarcoma that received a single SC infusion of carboplatin as adjunctive chemotherapeutic treatment following limb amputation or limb-sparing surgery. Design-Retrospective case series. Animals-17 client-owned dogs with spontaneously occurring osteosarcoma. Procedures-Medical records of dogs that underwent limb amputation or limb-sparing surgery followed by a single continuous SC infusion of carboplatin (total dose, 300 mg/m(2) infused over 3, 5, or 7 days) were evaluated. Signalment, tumor location, type of surgery (amputation or limb-sparing), duration of carboplatin infusion, results of hematologic and serum biochemical analyses, and adverse effects were recorded. Kaplan-Meier survival analysis was performed. Results-Median survival time for all dogs was 365 days. Nine dogs had adverse bone marrow-related (hematologic) effects, 1 had adverse gastrointestinal effects, and 7 had infections at the surgical site. No significant differences were detected in survival times of dogs grouped according to tumor location, type of surgery, duration of carboplatin infusion, or development of postoperative infection. Conclusions and Clinical Relevance-Median survival time and adverse effects in dogs with osteosarcoma that received a single SC infusion of carboplatin over a 3-, 5-, or 7-day period as adjunctive treatment following limb amputation or limb-sparing surgery were comparable to those of previously reported chemotherapy protocols requiring IV drug administration over several weeks. Further investigation is needed to evaluate the efficacy of this protocol as adjunctive treatment for osteosarcoma and other tumors in dogs.  相似文献   
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It has been the, policy of this laboratory to request that blood samples for inorganic phosphate analysis be collected in potassium oxalatesodium fluoride anticoagulant but the origin of this recommendation is obscure. The recommendation is presumed to be based on the premise that fluoride ion inhibits a wide range of enzymes that hydrolyse phosphate esters and release phosphate ions.  相似文献   
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For the past decade, supermarket chains have been positioned as the pre-eminent actor in global and national food systems. Some agri-food scholars argue that their ever-expanding transnational supply chains have established an era of stable production-consumption relations (or Food Regime), while others point to the conflicts they are encountering with governments, social movements and ‘alternative’ consumers. However, remarkably little attention has been paid to their relationship with communities and to community system sustainability. Based on fieldwork conducted in the Goulburn Valley, Australia, we argue that supermarket operations are contributing to community tensions through contestation over valued symbols and narratives about what desirable ‘progress’ looks like. We identified three interrelated points of tension being intensified by supermarket chains: whether progress is encapsulated by being an agricultural production or a modern consumption centre; whether progress should be based on a model of corporate capital or the local small business; and to what extent modern citizens can and should support community shopping instead of convenience shopping. For long-time residents, supermarkets are paradoxical actors appealing to, as well as, challenging the narrative of a community whose economic strength was based on the surrounding natural environment and local people’s endeavours. The concepts of solastalgia and structural nostalgia are relevant, with the former referring to the place-based distress experienced by residents whose local area is changing profoundly and the latter describing a process amplifying that distress. Through exploring the political paradoxes of community solastalgia, we raise new questions about supermarket authority within contemporary Food Regimes.  相似文献   
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ABSTRACT: Experimental infections of Sminthopsis crassicaudata, the fat-tailed dunnart, a carnivorous marsupial widely distributed throughout the arid and semi-arid zones of Australia, show that this species can act as an intermediate host for Neospora caninum. In contrast to existing models that develop relatively few N. caninum tissue cysts, dunnarts offer a new animal model in which active neosporosis is dominated by tissue cyst production. The results provide evidence for a sylvatic life cycle of N. caninum in Australia between marsupials and wild dogs. It establishes the foundation for an investigation of the impact and costs of neosporosis to wildlife.  相似文献   
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It is not always possible to identify female cats that have undergone previous ovariohysterectomy based on physical examination alone. An easy, cost‐effective method for screening female cats for reproductive status would be helpful for avoiding unnecessary exploratory laparotomies. The purpose of this prospective study was to compare diagnostic sensitivities of digital radiography, ultrasonography, and positive contrast vaginourethrography for determining reproductive status in female cats. Sixty‐seven recently euthanized female cats of unknown medical history and reproductive status were randomly selected and included in the study. Digital abdominal radiography, digital abdominal radiography with compression, abdominal ultrasonography, and positive contrast vaginourethrography were performed in sequence by a board‐certified veterinary radiologist and a second‐year radiology resident. Immediately following diagnostic imaging procedures, necropsy was performed. Ultrasonography of the uterus had the highest sensitivity (86%) for determining reproductive status of all the imaging modalities tested. The specificity was 88%, and the positive predictive value and negative predictive value were 96% and 68%, respectively. The calculated sensitivities and specificities of other modalities were as follows: digital radiographs (28%, 100%), digital compression radiographs (58%, 100%), and vaginourethrography (32%, 100%). Based on McNemar's test statistic, there was a significant difference in the sensitivity of ultrasound compared to digital radiographs (P ≤ 0.05), compression radiographs (P ≤ 0.05), and vaginourethrogram (P ≤ 0.05). Findings from the current study indicated that ultrasonography is a sensitive diagnostic test for determining reproductive status in female cats. Although more readily available in private practice and shelters, digital radiography and vaginourethrography are not reliable predictors of reproductive status.  相似文献   
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Salt tolerance of mature Williams Bon Cretien pear trees was assessed in a field trial on a duplex, slowly permeable clay loam. The trees were irrigated with a range of salinities; electrical conductivity of irrigation water (ECw) of 0.2 to 1.4 dS/m by flood for seven years or 0.2 to 2.1 dS/m by microjet sprinklers for nine years. Water-table levels were maintained below 3 m by a groundwater pump. Yield and leaf ion content were assessed during the treatment period. Aspects of growth and physiology were monitored in the 0.2 and 2.1 dS/m microjet treatments during the seventh irrigation season.Soil profile salinities varied between 3.0 and 4.3 dS/m for the most saline flood treatment and from 1.5 to 2.6 dS/m for the most saline microjet treatment. Soil sodicity (sodium absorption ratio) increased during the experiment, reaching a maximum of 9 in the most saline treatments. The salinity treatments caused reduced yields after seven years. In the most saline treatment (ECw = 2.1 dS/m, microjet-irrigated), yield decreased to about 60 and 50% of the control in the eighth and ninth years, respectively, and 40% of trees were dead in the ninth year. Leaf ion concentrations (in January) of the most saline treatment were at excess levels (>0.1% Cl and >0.02% Na) from 1982 to 1990. There were significant (P<0.01) negative linear relationships between yield in 1990 and leaf Na and Cl, measured both in 1990 and in 1989. During the seventh season of saline irrigation, lateral shoot growth was reduced, leaves and fruit were smaller and leaf fall was earlier in the 2.1 dS/m treatment compared with the control. Dawn and midday water potential and osmotic potential were not significantly affected by saline irrigation. Midday CO2-assimilation rates (A) and leaf conductance to water vapour diffusion (g) were similar for 2.1 dS/m irrigated and control trees, however there was a trend towards a reduction in A and g of these salt-treated trees late in the irrigation season when leaf Na and Cl had increased to 250 and 240 mM (tissue water basis) respectively.  相似文献   
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Canine viral plaques are uncommon skin lesions that are induced by papillomaviruses (PVs). Plaques are usually of little clinical significance in dogs, although they have been reported rarely to progress to squamous cell carcinoma (SCC). Here is described a 7‐year‐old mixed‐breed dog that developed numerous darkly pigmented plaques up to 8 cm in diameter. Multiple ulcerated nodular masses were visible within plaques on the ventrum and axilla. The dog showed no clinical evidence of immunodeficiency and appeared otherwise healthy. Over the next 2 years, five surgeries were performed to remove 23 ulcerated masses that ranged in size from 2 to 5 cm in diameter. Five masses were submitted for histology, and all were SCCs. Each was surrounded by epidermis that contained histological features consistent with those described in canine plaques. Suggestive of a PV aetiology, massive numbers of large keratohyaline granules were present throughout the thickened epidermis. Additionally, koilocytes were focally present, and one sample contained a band of keratinocytes within the superficial epidermis that contained pale cytoplasm and marginated chromatin. From two samples, DNA sequences from a previously unreported PV were amplified, and immunohistochemistry confirmed the presence of PV antigen in both. The PV DNA sequences were most similar to those of canine PVs previously associated with plaque formation. The plaques observed in this case were unusual owing to their rapid growth, large size and frequent malignant transformation. It is unknown whether this unusual behaviour was due to the specific PV detected in this case or to host factors within the dog.  相似文献   
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