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Darpa Saurav Jyethi P.S. Khillare Sayantan Sarkar 《Urban Forestry & Urban Greening》2014,13(4):854-860
This study reports, for the first time, the profiles and source analysis of 16 US EPA priority polycyclic aromatic hydrocarbons (PAHs) associated with PM10 (particulate matter with aerodynamic diameter ≤10 μm) at a protected and ecologically sensitive area – the Yamuna Biodiversity Park – located in the megacity Delhi, India. Weekly PM10 sampling was carried out at this location for 1 year (2009–2010) and the annual mean PM10 level was found to be ～9 times the World Health Organization limit. Seasonal variation of PAHs (range 37.2–74.0 ng m?3) was significant with winter values being 72% and 68% higher than summer and monsoon respectively. Principal component analysis coupled with multiple linear regression identified diesel, natural gas and lubricating oil combustion (49.5%), wood combustion (25.4%), gasoline (15.5%) and coal combustion (9.6%) sources for the observed PAHs. Heavy traffic on the national highway and arterial roads and domestic emissions from suburban households in the vicinity of the park appeared to have significantly affected its air quality. A substantial portion (～55%) of the aerosol PAH load was comprised of carcinogenic species, which yielded a considerably high lifetime inhalation cancer risk estimate (8.7E?04). If considered as a conservative lower-bound estimate, this risk translates into ～211 excess cancer cases for lifetime inhalation exposure to the observed PAH concentrations in Delhi. 相似文献
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Successful reproduction is considered to be the major determining factor for a weedy population to establish, colonize and infest. The breeding systems and seed production of six weedy taxa of Bidens were studied at an experimental garden in Jiangsu province in China. The results from four treatments (open pollination, bagged capitulum, decapitated capitulum, and pollen supplement) indicated that, with the exception of Bidens pilosa var. radiata, all the other five Bidens taxa; namely, B. pilosa var. pilosa, B. pilosa var. minor, B. frondosa, B.bipinnata, and B. biternata, were self‐fertile with a high seed‐set in bagged capitula, and a high autofertility index. Including the trait of vegetative propagation in self‐incompatible B. pilosa var. radiata, all the six weedy taxa of Bidens had the capacity of uniparental reproduction. The pollen limitation values for all six Bidens taxa were low in the present study. However, the seed production of B. pilosa var. radiata is likely to decrease in the absence of sufficient pollinators, related to its high pollen contribution and potential pollen limitation indices. The seed production per plant and per capitulum of the three varieties of B. pilosa was the highest among the six taxa and might contribute to their serious weediness. The breeding system type and seed number per capitulum could be used as a classification reference for Bidens taxa. 相似文献
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Saurav Kumar R. P. Raman Kundan Kumar P. K. Pandey Neeraj Kumar B. Mallesh Snatashree Mohanty Abhay Kumar 《Fish physiology and biochemistry》2013,39(4):733-747
Argulosis hampers aquaculture production and alters the host physiology and growth. Azadirachtin is recognized as a potential antiparasitic agent against Argulus sp. The present study aimed to investigate the effect of different concentration of azadirachtin solution on haematological and serum biochemical parameters of Argulus-infested goldfish Carassius auratus. Ninety Argulus-infested goldfish were randomly divided into six equal groups. Fish of group 1–5 were treated with azadirachtin solution through bath of 1, 5, 10, 15 and 20 mg L?1 as T1, T2, T3, T4 and T5, respectively, and group 6 was exposed to 2 % DMSO solution without azadirachtin and considered as negative control T0?. Along with six treatment groups, a positive control T0+ of healthy goldfish free from Argulus infestation was also maintained. Parasitic mortality was evaluated after 3 days of consecutive bath treatment. After 7 days of post-treatment, the blood and serum were drawn from each of the treatment groups and haematological and serum biochemical parameters were evaluated. Total leucocyte count (TLC), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), blood glucose, total protein (TP), globulin, serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT) were significantly (p < 0.05) high in negative control group when compared with positive control group. It could be concluded that Argulus infestation altered marked haematological and serum biochemical parameters. However, in treated groups complete elimination of Argulus was found in T4 and T5 groups. Also significant (p < 0.05) reduction in haematological and serum biochemical parameters of all the treatment groups were recorded in comparison with negative control group. In addition, T4 and T5 groups showed significantly (p < 0.05) high superoxide dismutase (SOD), catalase, total erythrocyte count (TEC) and haemoglobin (Hb). However, higher mean corpuscular haemoglobin concentration (MCHC), blood glucose and lactate dehydrogenase (LDH) levels in T5 group revealed that higher concentration of azadirachtin have notable effects on activity of vital tissues function and physiology of the host. Argulus spp. from infested goldfish could be eliminated using bath treatment with solution of azadirachtin having concentration of 15 mg L?1 and that also shifted haematological and serum biochemical parameters towards homeostasis. 相似文献
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Antibacterial and Cytotoxic New Napyradiomycins from the Marine-Derived Streptomyces sp. SCSIO 10428
Zhengchao Wu Sumei Li Jie Li Yuchan Chen Kumar Saurav Qingbo Zhang Haibo Zhang Wenjun Zhang Weimin Zhang Si Zhang Changsheng Zhang 《Marine drugs》2013,11(6):2113-2125
Three new napyradiomycins (1–3) were isolated from the culture broth of a marine-derived actinomycete strain SCSIO 10428, together with six known related analogues napyradiomycin A1 (4), 18-oxonapyradiomycin A1 (5), napyradiomycin B1 (6), napyradiomycin B3 (7), naphthomevalin (8), and napyradiomycin SR (9). The strain SCSIO 10428 was identified as a Streptomyces species by the sequence analysis of its 16S rRNA gene. The structures of new compounds 1–3, designated 4-dehydro-4a-dechloronapyradiomycin A1 (1), 3-dechloro-3-bromonapyradiomycin A1 (2), and 3-chloro-6,8-dihydroxy-8-α-lapachone (3), respectively, were elucidated by comparing their 1D and 2D NMR spectroscopic data with known congeners. None of the napyradiomycins 1–9 showed antioxidative activities. Napyradiomycins 1–8 displayed antibacterial activities against three Gram-positive bacteria Staphylococcus and Bacillus strains with MIC values ranging from 0.25 to 32 μg mL−1, with the exception that compound 3 had a MIC value of above 128 μg mL−1 against Staphylococcus aureus ATCC 29213. Napyradiomycins 2, 4, 6, and 7 exhibited moderate cytotoxicities against four human cancer cell lines SF-268, MCF-7, NCI-H460, and HepG-2 with IC50 values below 20 μM, while the IC50 values for other five napyradiomycins 1, 3, 5, 8 and 9 were above 20 μM. 相似文献
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Kumar N Jadhao SB Jha AK Kumar K Chandan NK Akhtar MS Aklakur M Kumar S Rana RS 《Fish physiology and biochemistry》2012,38(5):1343-1353
A 2-month preliminary study was conducted to delineate the effect of dietary methyl donors (choline, betaine, and lecithin) on the growth performance and metabolic status of Labeo rohita fingerlings subjected to endosulfan alone and in combination with elevated temperature. Four iso-caloric and iso-nitrogenous diets viz. basal diet, betaine-supplemented diet, choline-supplemented diet and lecithin-supplemented diet were prepared and fed to the different experimental groups throughout the experimental period as per the design. Two hundred and seventy fingerlings (average weight 7.95?±?0.04?g) were randomly distributed in six treatment groups each having three replicates. The experimental groups were as follows: fish subjected to normal water (without endosulfan) and fed with control diet (control group T(0)), fish subjected to endosulfan-treated water and fed with control diet (T(1)), fish subjected to concurrent exposure of endosulfan and elevated temperature and fed with control diet (T(2)), fish subjected to endosulfan and elevated temperature and fed with choline-supplemented diet (T(3)), fish subjected to endosulfan and temperature and fed with betaine-supplemented feed (T(4)), and fish subjected to endosulfan and temperature and fed with lecithin-supplemented feed (T(5)). The result shows that in both the groups, that is, endosulfan exposed and concurrent exposure to endosulfan and elevated temperature group of L. rohita the growth performance like percentage weight gain, feed conversion ratio and specific growth rates were significantly different (P?相似文献
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Kumar N Jadhao SB Chandan NK Kumar K Jha AK Bhushan S Kumar S Rana RS 《Fish physiology and biochemistry》2012,38(4):989-1000
A five-week experiment was conducted to delineate stress-mitigating effects of three different methyl donors in Labeo rohita fingerlings subjected to endosulfan toxicity. Four iso-nitrogenous and iso-caloric feed were prepared with and without supplementation of methyl donors. The feed were basal or control diet (i.e., without methyl donor supplementation), feed supplemented with choline, feed supplemented with betaine and feed supplemented with lecithin. Two hundred and twenty-five fishes were distributed randomly in five treatment groups each with three replicates. The experimental setup were normal water (without endosulfan) and fed with control diet (control group), endosulfan-treated water and fed with control diet (T?), endosulfan-treated water and fed with choline supplemented feed (T?), endosulfan-treated water and fed with betaine supplemented feed (T?) and endosulfan-treated water and fed with lecithin-supplemented feed (T?). The level of endosulfan in endosulfan treated water was maintained at the level of 1/10 of LC??, that is, 0.2 ppb. During the experiment, growth performances, metabolic enzyme activity and histological examination were done to assess the effect of treatments. The growth performance (percentage weight gain, feed conversion ratio, specific growth rate and protein efficiency ratio) and nutrient digestibility were significantly different (P<0.01) in lecithin, betaine and choline fed group when compared to endosulfan-exposed group fed with basal diet. The liver LDH and MDH activity were significantly (P<0.01) improved in the groups fed with methyl donor supplemented diet. The liver AST and ALT, brain AChE and muscle ALT did not change with supplementation in the diet, but muscle ALT and G6PDH significantly (P<0.01) changed with supplementation. The gill and liver ATPase and intestinal ALP were significantly (P<0.01) noticeably changed in supplemented group. After endosulfan exposure, histopathology alter like slight large vacuolation in hepatocyte and lipoid vacuole were observed and with supplementation normal appearance of liver were observed. The chromosome aberration (karyotype) was observed in endosulfan-exposed group. The result obtained in present study concluded that inclusion of methyl donors, particularly lecithin and betaine, in feed as nutritional supplements has a potential stress-mitigating effect in L. rohita fingerlings. 相似文献
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