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选取年龄、胎次、品种基本一致的哺乳母猪20头,随机分成对照组和试验组,试验分两批进行,每批10头.在相同的环境条件下,母猪自由饮水,饲喂同一哺乳母猪料.母猪分娩后4 d内,采用原场饲养制度,逐步增加哺乳母猪采食量,第5天开始,对照组采用不限量饲喂,试验组采用限量和添加哺乳母猪补充料的饲喂方式,仔猪21日龄断奶.结果显示两组仔猪21日龄的断奶窝重、母猪断奶后再发情时间和母猪哺乳失重等差异均不显著(P>0.05). 相似文献
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B. Birzele A. Meier H. Hindorf J. Krämer H.-W. Dehne 《European journal of plant pathology / European Foundation for Plant Pathology》2002,108(7):667-673
Details of our long-term research programme concerning the epidemiology of Fusarium spp. and mycotoxin production are summarized. Evaluation of the occurrence of Fusarium spp., mainly on winter wheat (Triticum aestivum), was carried out by investigating Fusarium infection and mycotoxin contamination. Two to 15% of grains were infested during 1995–1998 at three climatologically differing localities of the Rhineland, Germany. Disease progress was accelerated by rainfall during the flowering season. The species most frequently isolated were Fusarium avenaceum, F. poae, F. culmorum and F. graminearum. The mean deoxynivalenol (DON) content varied from 19gkg–1 (1995) to 310gkg–1 (1998) and was not always correlated with disease severity. Organic farming systems showed lower rates of infection with ear blight and lower mycotoxin contamination than conventional farming systems. 相似文献
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Joachim Dehne Kurt Skiebe Martin Stein Hagen Herdam Rolf Franke Heinz Leike 《Genetic Resources and Crop Evolution》1988,36(1):247-274
Zusammenfassung Für die Pflanzenzüchtung ist die genetische Variabilität eine entscheidende Voraussetzung. Sie basiert auf Genmutationen, Chromosomenmutationen, Introgressionen, Autopolyploidie, Alloplasmie, transponiblen DNA-Elementen und der Kombination. Diese Variabilitätskomponenten werden charakterisiert. Außerdem wird anhand von Beispielen ihr Einfluß auf die Entwicklung der Kulturpflanzen demonstriert.Um effektiv in der Pflanzenzüchtung arbeiten zu können, bedarf es der Analyse von genetischer Variabilität. Dies geschieht mit Hilfe von Kreuzungsexperimenten und Parameterschätzungen. In zunehmendem Maße werden auch Marker bei der Analyse eingesetzt.Einen wichtigen Faktor zur zielgerichteten Beeinflussung der genetischen Variabilität stellt die Selektion dar. Sie führt zur Einschränkung der Kombination. Die Selektion ist für die Entwicklung von Sorten notwendig. Sie hat aber auch eine Verarmung an genetischer Information zur Folge.Für den weiteren Fortschritt in der Pflanzenzüchtung gilt es, eine genügende genetische Variabilität zu gewährleisten. Dazu bedarf es auch Maßnahmen zu ihrer Erhaltung. Genetische Variabilität muß außerdem verstärkt charakterisiert und analysiert werden.
Genetic variability
Summary Genetic variability is a decisive prerequisite to plant breeding. It is based on gene mutations, chromosome mutations, introgressions, autopolyploidy, alloplasmy, transposible DNA elements and recombinations. These components of the genetic variability are illustrated and examples are given to demonstrate their contributions to the evolution of crop plants.Effective plant breeding requires the analysis of the genetic variation. It is analysed by hereditary studies and parameter estimations. The utilization of markers is continuously increasing in studies of the genetic variation.Selection is an important factor to a purposive influence on the genetic variability and leads to a restriction of the recombinations. Selection is necessary for the development of varieties but results also in an impoverishment of genetic information.For the continuous progress in plant breeding an adequate genetic variability is to secure. That includes also activities to its conservation. Furthermore the characterization and analysis of the genetic variability has to be intensified.
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Induced resistance to the apple scab fungus Venturia inaequalis was demonstrated in greenhouse tests with 12-day-old seedlings of the apple cultivar Golden Delicious treated with methyl 2,6-dichloro-isonicotinate or 3,5-dichlorosalicylic acid prior to inoculation with the causal fungus. Studies of the dose-response of flusilazole on induced resistant plants revealed synergistic effects between both crop protection principles. Therefore, the use of such resistance-inducing compounds in the field might allow a reduction in the number of fungicide applications, and possibly a reduction in dose, thus resulting in improved efficacy of fungicides. There was also evidence that induced resistance could prove to be a valid strategy for the treatment of pathogen populations with reduced sensitivity to a given fungicide. © 1998 SCI 相似文献
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文章着重分析了内蒙古当前林业发展形势及国民经济发展对林业的需求 ,并客观的论述了自治区林业发展的有利条件和制约因素 ,希望能够对今后的林业建设有所帮助 ,从而把握机遇 ,加快环境建设步伐 ,早日改善环境状况。 相似文献
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HW Michelmann D Rath E Töpfer-Petersen P Schwartz 《Reproduction in domestic animals》2007,42(6):594-602
Porcine oocytes and pre-implantation embryos from the same, as well as from different animals, have an extremely heterogeneous morphology of the zona pellucida (ZP) surface, as shown by scanning electron microscopy. For years, it has been believed that this heterogeneous morphology plays an important role in the sperm-oocyte interaction. The aim of this study was to analyse the zona morphology and sperm-binding patterns on the porcine ZP. Oocytes were divided into four categories: immature, matured in vivo, or matured in vitro over a time period of 24 or 48 h. The zona morphology of early embryos grown in vivo or in vitro was also investigated. Four different types of zona morphology were detectable. They ranged from a porous, net-like structure to a nearly smooth and compact surface. No correlation could be established between the different kinds of maturation in terms of these zona types. All oocytes exhibited extremely heterogeneous zona morphologies, with no clear trend. During subsequent in vivo embryo development, the zona surface changes from a porous structure to one with a compact surface, while the morphology of in vitro embryos remained compact at all stages of development. The analysis of the number and distribution patterns of spermatozoa trapped in the ZP revealed extremely variable patterns, regardless of the zona morphology. Differences were only present if sorted or unsorted spermatozoa were used for insemination. Regardless of the number of inseminated spermatozoa after sorting, only a few (1-2) could be detected on the ZP. Whether oocytes were matured in vivo or in vitro was not a relevant factor. Unsorted spermatozoa bound in higher numbers than sorted ones. The number was directly dependent on the number of spermatozoa used for insemination. 相似文献
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Adhesion to the host surface is the first step for successful plant pathogen development and has been reported to be associated with both passive and active processes. For conidia of Venturia inaequalis, which depend on leaf wetness for germination, this process has not yet been described. Conidia of V. inaequalis adhered to wet hydrophobic surfaces immediately after contact to the surface, hours before initiation of germination. Attachment of nongerminated conidia was much better on hydrophobic surfaces, such as apple leaves and polystyrene, than on hydrophilic glass. Conidia released adhesive material localized in a droplet named spore tip glue (STG) at the spore apex which interacted with a contact surface only when water was present. Histochemical investigations indicated the presence of proteins and carbohydrates in STG, lectin labeling the presence of beta-galactose and N-acetylglucosaminyl residues. Transmission electron microscopy revealed two phases in the STG at the tip of dry mature conidia; as STG was present on the outer side of the intact fungal cell wall its formation should be associated with the secretion of glue through pores of the conidial wall. Surface-active substances affected the adhesion of conidia to hydrophobic surfaces stressing the importance of hydrophobic interactions. The use of protein biosynthesis inhibitors did not affect adhesion of conidia indicating that the adhesive material was preformed. It is concluded that the coincidence of STG, contact to a hydrophobic surface, and free water are essential for the adhesion of V. inaequalis conidia. 相似文献
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Leaf rust caused by the fungus Puccinia triticina is one of the most important diseases of wheat (Triticum aestivum) worldwide. The use of resistant wheat cultivars is considered the most economical and environment-friendly approach in controlling
the disease. The Lr38 gene, introgressed from Agropyron intermedium, confers a stable seedling and adult plant resistance against multiple isolates tested in Europe. In the present study, 94
F2 plants resulting from a cross made between the resistant Thatcher-derived near-isogenic line (NIL) RL6097, and the susceptible
Ethiopian wheat cultivar Kubsa were used to map the Thatcher Lr38 locus in wheat using simple sequence repeat (SSR) markers. Out of 54 markers tested, 15 SSRs were polymorphic between the
two parents and subsequently genotyped in the population. The P. triticina isolate DZ7-24 (race FGJTJ), discriminating Lr38 resistant and susceptible plants, was used to inoculate seedlings of the two parents and the segregating population. The
SSR markers Xwmc773 and Xbarc273 flanked the Lr38 locus at a distance of 6.1 and 7.9 cM, respectively, to the proximal end of wheat chromosome arm 6DL. The SSR markers Xcfd5 and Xcfd60 both flanked the locus at a distance of 22.1 cM to the distal end of 6DL. In future, these SSR markers can be used by wheat
breeders and pathologists for marker assisted selection (MAS) of Lr38-mediated leaf rust resistance in wheat. 相似文献
10.
The spatial pattern of Fusarium‐infected kernels and their mycotoxin contamination was studied in four wheat fields in Germany using geo‐referenced sampling grids (12–15 × 20–30 m, 28–30 samples per field) at harvest. For each sample, frequency of Fusarium‐infected kernels and spectrum of species were assessed microbiologically; mycotoxin contents were determined by HPLC‐MS/MS analysis. Spatial variability of pathogens and mycotoxins was analysed using various parameters including Spatial Analysis by Distance IndicEs (sadie® ). Microdochium majus, the most frequent head blight pathogen in 1998, was less frequent in 1999 and could not be detected in kernels from two fields in 2004. Fusarium avenaceum, F. graminearum and F. poae were the most frequent Fusarium species, with 7–8 species per field. The frequency of Fusarium‐infected kernels was 3–15% and the incidence of species showed considerable within‐field variability. Spatial patterns varied among Fusarium species as well as from field to field. Although pathogens and mycotoxin were often distributed randomly in the field, F. avenaceum, F. graminearum, F. poae, F. sporotrichioides, F. tricinctum and the mycotoxin moniliformin had an aggregated pattern in at least one field. Patterns are discussed in relation to spread of Fusarium species depending on inoculum sources, spore type, kind of dispersal, availability of susceptible host tissue and micro‐climate. Sampling of wheat fields for representative assessment of mycotoxins is complicated by random patterns of Fusarium‐infected kernels, especially where the frequency of infection is small. 相似文献