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1.
Cross sections of woody stems of 309 diseased grapevines collected in France showed two kinds of necrosis typical of esca: a) A central light-colored necrosis of soft consistency, consisting of three zones, preceded by a centrally discolored wood, and b) a sectorial light-colored necrosis composed of two zones preceded by a sectorial brown necrosis. Isolations showed that different microflora was associated with each necrosis. Phaeoacremonium aleophilum and Phaeoacremonium chlamydosporum occurred in the discolored wood and the zones bordering the central decayed wood. Eutypa lata was the main fungus isolated from sectorial brown necrosis and the zones adjoining the decay wood. Phellinus punctatus was isolated from the sectorial and central decayed wood. Stereum hirsutum was present in decayed wood of 15 grapevines with esca symptoms not inhabited by P. punctatus. Wood decay tests and pathogenicity tests showed that S. hirsutum and P. punctatus were responsible for the decayed wood. Phaeoacremonium chlamydosporum and S. hirsutum produced a centrally discolored wood similar to that found in esca-affected vines. Phaeoacremonium aleophilum caused a sectorial brown necrosis of soft texture. From these studies, it was found that esca is a complex disease involving several microorganisms whose role in the process leading to wood degradation is discussed.  相似文献   
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Toxic factors in enzymes used in laundry products   总被引:1,自引:0,他引:1  
Autolyzates of Bacillus subtilis used as enzymes in laundry products contain several potentially toxic substances.  相似文献   
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A collection of 55 Fusarium graminearum (Gibberella zeae) strains isolated between 1969 and 2009 in Belgium, Canada, Germany, Italy, Luxembourg, or the USA belonging to the three known chemotypes (3-acetylated deoxynivalenol, 15-acetylated deoxynivalenol and nivalenol) were screened for their sensitivity towards the fungicide trifloxystrobin using a liquid culture assay. None of the isolates was completely inhibited by trifloxystrobin concentrations up to 3 mM. For comparison, prothioconazole completely inhibited fungal growth of a standard isolate at concentrations as low as 0.007 mM. The maximum level of inhibition, which could be obtained by trifloxystrobin, ranged from 14 to 65% among the strains tested and was not significantly affected by the country of origin or by the chemotype. The absence of significant differences in resistance levels between the countries of origin and chemotypes as well as the fact that strains isolated before the market introduction of strobilurins in 1996 also showed a high level of resistance is evidence that this is largely a case of natural resistance and not primarily related to strobilurin use in agriculture.  相似文献   
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ABSTRACT Change in relative frequencies of the three main genetic types of Botrytis cinerea (Group I, Group II vacuma, and Group II transposa) were monitored over time from 1998 to 2000 in three Bordeaux vineyards not treated with fungicides. During 2000, Group I isolates, detected mostly at flowering comprised only 2.5% of the entire population. Within Group II, the complementary frequencies of vacuma and transposa isolates differed significantly depending on grapevine phenological stages and organs. Every year and at all sites, including one noble rot site, transposa isolates dominated at every stage, particularly on overwintering canes and at harvest (greater than 86.7% on berries). The complementary frequency of vacuma isolates reached a maximum on senescing floral caps (between 23.5 and 71.4%) and then decreased significantly until harvest on leaves and berries. In pathogenicity tests on grape berries, transposa isolates were significantly more virulent than were vacuma isolates. Mycelial growth rate was negatively correlated with virulence, notably on leaves in transposa and with double resistance to the fungicides carbendazim and vinclozolin. In vacuma, this double resistance was positively correlated with virulence on leaves. Change in the vacuma and transposa frequencies was most likely caused by differences in saprotrophic and pathogenic fitness. Possible interactions between fungicide resistance profiles and fitness are discussed.  相似文献   
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A third species of peccary, discovered in the Chaco of Paraguay, is added to the living members of family Tayassuidae. It is assigned to the genus Catagonus Ameghino, heretofore considered confined to the Pleistocene. The new peccary is conspecific with Catagonus wagneri (Rusconi), a species placed in the related extinct genus Platygonus LeConte when it was described from pre-Hispanic archeological deposits of Argentina.  相似文献   
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Landscape Ecology - Prioritising is likely to differ depending on the species considered for connectivity assessments, leading to a lack of consensual decisions for territorial planning. The...  相似文献   
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One hundred and twenty-one single-spore strains of Botrytis cinerea isolated from Bordeaux vineyards were molecularly characterized as either transposa or vacuma, two subpopulations of B. cinerea distinguished by the presence of transposable elements. Forty-three vacuma and 68 transposa strains were distributed into two main classes (mycelial or sclerotial) by morphological phenotype according to the organ of origin. Strains isolated from overwintering sclerotia produced exclusively sclerotial colonies. The mycelial growth rate of 21 transposa and 13 vacuma strains was significantly influenced by agar-medium and temperature. The mycelial growth rate was significantly strain-dependent at favourable temperatures (15, 20 and 25 °C), but not at limiting ones (5 and 28 °C): vacuma strains showed the fastest growth rates. The strains of the two subpopulations were similar in virulence on both host species tested (Vitis vinifera and Nicotiana clevelandii). The grapevine leaves were significantly more susceptible to B. cinerea than those of tobacco. A significant negative correlation was established between virulence and mycelial growth rate. The epidemiological consequences concerning population structure of B. cinerea in vineyards are discussed.  相似文献   
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