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1.
Phytoplasmas associated with lettuce phyllody (LP) and wild lettuce phyllody (WLP) in southern Iran were partially characterized by molecular analyses and host-range studies. Agents of both diseases were transmitted by Neoaliturus fenestratus , a leafhopper colonizing lettuce and wild lettuce, to lettuce, wild lettuce, sowthistle and periwinkle, but not to safflower, sunflower, calendula and sesame. Both phytoplasmas induced bud proliferation, virescence, phyllody and witches' broom in infected plants. Total DNA extracted from infected lettuce and wild lettuce or from vector tissues was subjected to PCR using phytoplasma-specific primer pair P1/P7 or nested PCR using P1/P7 followed by R16F2n/R16R2. PCR product of nested PCR (1·2 kbp) was subjected to restriction fragment length polymorphism (RFLP). RFLP analysis of nested PCR product identified the LP, WLP and N. fenestratus -associated phytoplasmas as members of the pigeon pea witches' broom group, 16SrIX. Phylogenetic analysis of the 16S rRNA gene sequence also clustered LP and WLP phytoplasmas with other known members of the 16SrIX group. While no significant differences could be detected between LP and WLP phytoplasmas, both isolates differed from Lebanese wild lettuce phyllody in molecular properties.  相似文献   
2.
Microbial indices and their spatial patterns are strongly affected by environmental factors. Spatial variability of soil properties is one of the most important causes of variability in soil microbial indices. This research was conducted in the Caspian forest to assess spatial variabilities and frequency distributions of microbial properties.Ninety soil samples were taken using a grid sampling design 40 9 40 m. Microbial indices, organic carbon,nitrogen and pH were determined. Soil variable distributions showed that microbial indices had abnormal distributions. Logarithmic transformation produced normal distribution. Spatial continuity using geostatistical(variogram) was studied and maps obtained by point kriging.The variograms revealed the presence of spatial autocorrelation. The results indicate that spatial dependence of soil microbial indices was affected by non-intrinsic factors and forest management procedures. The maps show that soil microbial indices and soil properties have spatial variability. The spatial pattern of microbial indices was correlated to organic carbon and nitrogen.  相似文献   
3.
This study aims to investigate the land cover changes in relation to traditional utilization and livestock grazing, socioeconomic development, and demographic changes during recent decades in a forested watershed in the southern Zagros, Iran. Data on land cover change were collected through stereo interpretation with digital photogrammetric techniques applied to sets of scanned aerial photographs from 1969 and 1993 with a systematic grid of sample plots provided by GIS techniques. Efforts were made to mitigate errors in the interpretation. Field data were gathered in 2003 from woodland inventory, biophysical, and demographic data and in 2006 from interviews. The results show stability in crown cover density (%) and in the number of large trees, and an increase in the woodland class. The results may be related to a change in woodland utilization. Socioeconomic development, decrease in human and livestock population, and a change in the type of livestock have influenced the traditional pattern. Steep slopes partially limit human accessibility to woodland resources. Around 54% of the oak trees are in coppice form and natural regeneration of the trees is rare. Overgrazing and seed gathering have a negative effect on regeneration and endanger the future survival of the woodland. Copyright © 2008 John Wiley & Sons, Ltd.  相似文献   
4.
In the present study, naringin, a flavonoid isolated from the grape and citrus fruit species, was incorporated with poly(ε-caprolactone)/gelatin composite mats in order to develop a potential wound dressing. The composite mats were prepared by electrospinning of poly(ε-caprolactone)/gelatin (1:1 (w/w)) solution incorporated with 1.50 %, 3 % and 6 % (w/w) of naringin. The electrospun mats were evaluated regarding their morphology, contact angle, water-uptake capacity, water vapor transmission rate, tensile properties, drug release, cellular response and in vivo wound healing activity. The study showed that after 2 weeks, the full-thickness excisional wounds of Wistar rats treated with the naringin-loaded dressings achieved a wound closure of higher than 94 % and the dressing containing 6 % (w/w) naringin had almost 100 % wound closure. The sterile gauze, as the control group, showed nearly 86 % of wound closure after this period of time. Our results provided evidence that supports the possible applicability of naringin-loaded wound dressing for successful wound treatment.  相似文献   
5.
Ornithobacterium rhinotracheale is a gram negative bacterial pathogen causing respiratory tract infections in poultry. Tracheal, lung and serum samples were obtained from 21 broiler flocks of 8 farms from a slaughterhouse located in south-eastern of Iran. Among 630 tracheal and lung samples from samples resulting from 315 chickens, 11 (3.5%) ORT isolates were identified using biochemical tests. The isolates originated from 9 (42.9%) flocks out of 4 farms. All of the isolates were recovered from tracheal swabs and showed an API 20NE identification biocode 0-2-2-0-0-0-4. Of the 420 serum samples examined by ELISA, 134 (31.9%) sera from 17 (81.0%) flocks were positive for ORT antibodies. These results indicate that ORT is present in most broiler flocks with respiratory disorders in southeast Iran.  相似文献   
6.
ABSTRACT

Litopenaeus vannamei postlarvae were exposed to 0, 6, 13, and 19 mg/L total ammonia nitrogen (TAN) treatments. After 45 days, shrimp weight and length were lowest under TAN concentrations of 13 and 19 mg/L (P ≤ 0.05). Maximum weight gain was observed in control and 6 mg/L treatments. Mortality was highest (80.55 ± 4.80%) under 19 mg/L reared in 35 ppt salinity. Average intermolt periods of PLs exposed to 0, 6, 13, and 19 mg/L TAN were 11.5 ± 0.7, 10.8 ± 1.3, 9.4 ± 1.0, and 8.7 ± 0.6 days under 35 ppt and 11.1 ± 0.5, 10.7 ± 0.6, 10.1 ± 0.5, and 9.5 ± 0.2 days under 45 ppt salinity. Although TAN increased postlarvae molting frequency, its negative effects on the shrimp growth and survival of PLs was directly linked to its concentration and exposure duration. Higher salinity reduces the effects of ammonia and increases the survival.  相似文献   
7.
Research has shown that fresh fish is an extremely perishable food as compared to other food commodities. In this study, quality deterioration during storage of golden grey mullet (GGM) iced for 0, 6, and 12 h after landing was monitored. Microbiological indices (total viable count, TVC; psychrophilic counts, PTC; lactic acid bacteria, LAB; and Enterobacteriaceae counts, EBC), chemical parameters (pH; total volatile basic nitrogen, TVB-N; peroxide value, PV; thiobarbituric acid, TBA; and free fatty acids, FFA) values increased over the preicing duration. Delayed icing did not lead to significant increases (p > 0.05) in TVC, PTC, LAB, and EBC throughout the period of storage. All chemical indices increased during storage (p < 0.05). This study showed that sensorial analysis of GGM correlated well with microbiological analysis. Results of this study based on the microbiological and raw fish sensorial data indicated that the shelf life of GGM stored in ice immediately after catch was approximately 10–11 days, while the 6- and 12-h delays in icing shortened the shelf life to approximately 6–7 and 2–3 days, respectively.  相似文献   
8.
Salinity is one of the major agricultural problems in arid and ?semi-arid regions. Considering the variation of plant’s ?sensitivity to salinity during growth, a greenhouse study with completely randomized design? was conducted to determine the relative salinity tolerance of Quinoa (Chenopodium quinoa Willd.) at different growth stages from seedling establishment to maturity (establishment, flowering and seed filling) by evaluating the Salinity Threshold Value (STV). Eight levels of ECi (i.e., Non-saline, 2, 4, 8, 12, 15, 20, 25 dSm?1) with four replications and five levels (i.e., Non-saline, 10, 15, 20, 25 dSm?1) with three triplications were applied at first and two last growth stages, respectively. A ?comparison was performed on some growth and yield parameters of plants irrigated by considering STV (T) and ?plants irrigated permanently by mentioned salinity levels regardless of STV (P) to choose ?which method (P or T) is better at each salinity level. The STV was ?evaluated 8, 20 and 15 dSm?1 at each growth stage, respectively. Seedling of Quinoa was more sensitive to salinity than the mature plant. Therefore, after establishment Quinoa has the ?feasibility of irrigation by high-saline waters. The (P) ?method was suitable only if the freshwater was available during all growth period of the plant; otherwise at higher salinities irrigation should be performed by considering STV (T method) to minimize the intensity of growth and yield reduction and to prevent yield loss at very high salinities. To achieve this, if high-saline water is available it’s possible to ?use plant propagation techniques or cultivating Quinoa simultaneously with seasonal rainfall.?  相似文献   
9.
Topography has an important influence on the distribution of soils and their properties, especially in hilly lands, and related data are easily available, measurable and recognizable from digital elevation models (DEMs). To our knowledge, little attention has previously been paid to the effect of DEM attributes on the distribution of soils, using ordination methods. The objective of this study was to analyze relationships between topographical properties derived from DEM and soil distribution and to discuss their applicability in Digital Soil Mapping (DSM). The study was carried out in the Borujen area of central Zagros, Iran. A total of 13 plots (each one of 6.75 ha) were set up to calculate the percentages of the dominant soil series. Fifteen DEM attributes, including slope, aspect, curvature, maximum and minimum curvature, planform curvature, profile curvature, tangent curvature, wetness index, power index, sediment index, area solar radiation, direct radiation, diffuse radiation and direct duration were also computed. Canonical Correspondence Analysis (CCA) was used to summarize the data set and to evaluate the expected relationships. The results obtained show that there was a relatively strong correspondence between soils' series distribution and topographical properties. The DEM attributes that best related to the first axis were maximum curvature, slope and sediment index, all of which significantly positive correlated, and wetness index, direct duration and minimum curvature, all of which were negatively related. The second axis showed a negative trend with wetness index, direct duration and aspect, and a positive trend with sediment index and slope. These gradients were closely related to the first three canonical axes and explained 71.8% of the total variance of the soil series. The residual variance (28.2% of the total variance) was related to other soil forming factors, like parent material and vegetation cover, which were not investigated in this study. Considering that DEMs are still the most important source of environmental information, understanding the role of topographical factors in a region should help us to identify soils and their properties better and enable us to apply these derivates as input data in DSM.  相似文献   
10.
Crop residues are beneficial substances affecting crop production and soil properties. A field experiment was carried out to evaluate the effects of wheat (Triticum aestivum L.) residue rates (0, 25, 50 and 75%) combined with N levels (0, 34.5, 69, 103.5 kg ha?1) on yield and yield components of two red common bean (Phaseolus vulgaris L.) cultivars and to monitor chemical soil parameters. The experiment was conducted at Research Center, College of Agriculture, Shiraz University, Shiraz, Iran for two years (2008–2009). The experiment was conducted as a split–split plot arranged in a randomized complete blocks design with three replications. The highest seed yield was obtained when 25–50% of residues were incorporated. The highest seed yield, seed weight per plant, 100-seed weight and seed number per pod were obtained with 103.5 kg N ha?1 with no significant difference to 69 kg N ha?1. Residue incorporation significantly increased soil organic carbon (SOC) as well as available K and P content. It is possible to sow red common bean as a double cropping by soil incorporation of 25–50% wheat residues with application of 69 kg N ha?1.  相似文献   
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