黑胫病菌（Leptosphaeria biglobosa）在油菜叶片和茎中的侵染过程观察     

史志丹  宋培玲  郝丽芬  皇甫海燕  燕孟娇  杨永青  吴晶  赵丽丽  李子钦 《中国油料作物学报》2021,43(2):286-292

为明确黑胫病菌（Leptosphaeria biglobosa）在甘蓝型油菜叶片和茎中的侵染及扩展过程，利用绿色荧光蛋 白（GFP）标记的黑胫病菌株接菌油菜叶片，利用激光共聚焦显微镜观察菌株在油菜叶片和茎中的侵染过程。结果 表明，接种油菜叶片7 h后，分生孢子萌发并长出芽管；17 h后，芽管侵入气孔；24 h后，分生孢子全部萌发；36 h后萌 发的芽管形成菌丝；120 h后，菌丝在叶片表皮细胞间隙蔓延，并侵入叶肉细胞。13 d后，菌丝侵入茎部皮层组织； 15 d后，菌丝在皮层细胞间隙蔓延，并侵染至茎表皮；21 d后，菌丝侵染至维管组织；23 d后，菌丝侵染至茎韧皮部； 25 d后，茎导管被侵染，并向木质部扩展。本研究发现的L. biglobosa 在油菜叶片和茎中的侵染过程，可为油菜与黑 胫病菌互作的研究、黑胫病致病机理及防治提供参考。  相似文献   

Effect of GYY4137 on cytosolic lipid decomposition in mouse primary hepatocytes     

WANG Hong-gang  ZHANG You-jing  FU Chao-shen  WANG Jun  WU Dong-dong  ZHONG Pei-yu  WANG Guo-ying  LI Yan-zhang 《园艺学报》2017,33(8):1520-1523

AIM: To evaluate the effect of GYY4137, a novel hydrogen sulfide (H₂S) donor, on cytosolic lipid decomposition in mouse primary steatosis hepatocytes. METHODS: Oleic acid (OA) was used to induce hepatic steatosis model in vitro. The C57BL/6 mouse primary hepatocytes isolated and cultured by 2-step in situ perfusion were divided into 4 groups:the cells in control group were incubated with normal medium for 54 h; the cells in model group were incubated with OA at 1.2 mmol/L for 48 h followed by serum-free phenol red-free RPMI-1640 for 6 h; the cells in H₂S group or DL-propar-gylglycine (PAG; an inhibitor of cystathione γ-lysase, inhibiting H₂S synthesis) group were incubated with OA at 1.2 mmol/L for 48 h followed by serum-free phenol red-free RPMI-1640 which contained 1 mmol/L GYY4137 or 200 μmol/L PAG for 6 h. The glycerin release and the protein expression of hormone-sensitive lipase (HSL) in the cells were mea-sured. RESULTS: Compared with model group, the glycerin release and the protein expression of phosphorylated HSL (p-HSL) in H₂S group decreased significantly, while those increased significantly in PAG group. CONCLUSION: In steatosis hepatocytes, exogenous H₂S possibly decreases cytosolic lipid decomposition by decreasing the protein level of p-HSL.  相似文献   

南水北调东线淮安段里运河输水水质风险控制机制研究与应用     

皇甫全欢  王道虎  谢亚军 《国际沙棘研究与开发》2017,(11)

南水北调东线工程实施后,自2013年正式通水,近4年的运行,目前已为山东调水近19亿m3,工程始终处于安全稳定状态,输水干线水质按规定稳定达到地表水Ⅲ类标准,13个设区市的规划供水范围目标全部实现.但淮安段里运河穿越城区,河道沿线情况复杂,存在水质污染的风险.本文旨在通过对里运河沿线风险源进行分析研究,并对各风险源提出相应的可能解决方案,以保证调水水质稳定达标.  相似文献   

Effect of exogenous hydrogen sulfide on lipophagy in mouse primary hepatocytes     

WANG Hong-gang  ZHANG You-jing  HUANGFU Chao-shen  CHEN Ming-liang  WANG Jun  WU Dong-dong  XIE Zhen-xing  LI Yan-zhang  JI Ai-ling 《园艺学报》2017,33(10):1901-1905

AIM: To evaluate the effect of exogenous hydrogen sulfide (H₂S) from GYY4137 on lipophagy in mouse primary hepatocytes. METHODS: The C57BL/6 mouse primary hepatocytes isolated and cultured by 2-step in situ perfusion method were divided into 4 groups: the cells in control group were incubated with normal medium; the cells in model group were incubated with 1.2 mmol/L oleic acid (OA) for 48 h; the cells in H₂S group or propargylglycine (PAG) group were incubated with 1.2 mmol/L OA for 48 h followed by serum-free phenol red-free RPMI-1640 medium which contained 1 mmol/L GYY4137 or 200 μmol/L PAG for 6 h. The cells were collected to conduct immunofluorescence staining of LC3 and photography under fluorescence microscope, phase-contrast microscope or transmission electron microscope. The protein expression of LC3-Ⅰ/Ⅱ in the hepatocytes was determined by Western blot. RESULTS: In contrast with the model group, the fluorescent particles of LC3, the protein expression of LC3, the number of autophagic lysosome and vacuoles in hepatocytes in H₂S group increased. CONCLUSION: In steatosis hepatocytes, exogenous H₂S promotes the lipophagy.  相似文献   

不同部位西门塔尔牛肉理化品质分析及烤制加工适宜性的研究     

不同部位西门塔尔牛肉理化品质分析及烤制加工适宜性的研究 《畜牧与饲料科学》2021,42(3):97-101

[目的]研究不同部位西门塔尔牛肉原料肉的理化品质,评价烤制加工后的食用品质和感官品质。[方法]选取体重为550 kg左右的西门塔尔肉牛10头,屠宰分割后取不同部位牛肉（里脊、外脊、前腿、后腿、眼肉、米龙）。4 ℃排酸72 h后,采用常规方法测定不同部位牛肉的蛋白质含量、脂肪含量、水分含量、pH值以及色泽指标,并对大理石花纹等级进行评分;220 ℃烤制10 min后,对不同部位牛肉进行剪切力和加工损失率测定,并对感官品质指标进行评价。[结果]不同部位牛肉的脂肪含量、大理石花纹评分、L^*值、a^*值、剪切力、色泽评分、嫩度评分、多汁性评分、香气评分和总体可接受性评分差异显著（P<0.05或P<0.01）,不同部位牛肉中的蛋白质含量、水分含量、pH值、b^*值、加工损失率、滋味评分差异不显著（P>0.05）。眼肉中脂肪含量最高（P<0.01）,前腿、后腿脂肪含量相同,均为最低（P<0.01）;眼肉、外脊的大理石花纹评分显著（P<0.01）高于其他部位;眼肉、外脊的L^*值显著（P<0.05）高于其他部位,里脊、后腿的L^*值显著（P<0.05）低于其他部位;外脊和眼肉的a^*值显著（P<0.01）低于其他部位;眼肉、外脊的剪切力显著（P<0.01）低于其他部位;眼肉的总体可接受性评分显著（P<0.05）高于其他部位,前腿、后腿和米龙的总体可接受性评分相同,均为最低（P<0.05）。[结论]外脊、眼肉的肌内脂肪含量高于其他部位,烤制后产品的嫩度、滋味及总体可接受性较好。眼肉和外脊是西门塔尔牛肉中最适宜加工烤制产品的部位。  相似文献   

Morphological disparities in the epidermal and anatomical features of the leaf among wild Brassica juncea populations     

CHAOHE HUANGFU  XIAOLING SONG  SHENG QIANG 《Weed Biology and Management》2009,9(3):234-242

In the present article, we investigated the epidermal and anatomical features of 10 wild Brassica juncea populations with different susceptibilities to glyphosate in detail. The characteristics of the leaf surface and anatomy could affect the efficacy of glyphosate uptake in weeds through the influence of its adherence and penetration. Our results showed that herbicide-resistant wild populations generally had greater leaf thickness, adaxial cutin thickness, and trichome density in the upper epidermis, but lower spongy tissue thickness and vascular bundle numbers, as compared with the susceptible populations, suggesting that differences in morphological traits among populations might contribute to their variable response to glyphosate. The possible morphological involvement in the differential susceptibility of wild B. juncea populations to glyphosate also was discussed.  相似文献   

一例奶牛皱胃溃疡伴低血钾综合征的临床治疗     

石冬梅  张华  皇甫和平  郭永刚 《中国畜牧兽医》2007,34(3):35-37

2005年12月郑州牧业工程高等专科学校附属兽医院接诊了1例皱胃溃疡伴低血钾综合征的奶牛，该牛同时患有低血钙、酮病等。病情发展中患牛在血钾含量为2.28 mmol/L时四肢无力，卧地不起，通过补钾及综合治疗血钾升至3.45 mmol/L时又能自主站立，对皱胃溃疡采取消炎、抑制胃酸分泌、收敛止血等方法进行治疗。  相似文献   

升金湖消落带植被特征及与土壤特性的关系     

张黎明  皇甫超河  远勇帅  孟盈盈  贾璇 《草业科学》2022,38(1):52-62

探讨湖泊湿地土壤特性对植物多样性的影响,对于理解湿地植被分布及功能的维持机制具有重要意义.在安徽升金湖消落带沿水位梯度设立研究样方,测定植物的盖度、地上生物量以及土壤含水量、有机碳、全氮、全磷等指标,并结合Pearson相关性分析、冗余分析(redundancy analysis,RDA)探讨了土壤特性对植物多样性的影响.结果表明,随着地下水位上升,土壤含水量与全氮含量显著增加(P<0.05),土壤有机碳和土壤全磷含量显著降低(P<0.05).土壤有机碳、全磷含量以及C?N与土壤含水量极显著负相关(P<0.001),土壤全氮、N?P以及C?P与土壤含水量极显著正相关(P<0.001).土壤含水量、土壤全氮含量以及C?P和N?P是影响消落带植被物种多样性变化的关键因子,共同解释了变化的91.85％.总之,土壤含水量可以直接影响消落带植物多样性,也可以通过改变土壤中氮元素的转化和磷元素的有效性间接影响物种多样性.  相似文献   

种植密度对洛薯8号生物学性状及产量的影响     

皇甫庭  李艳梅 《河南农业科学》2014,43(7)

以洛薯8号为材料,采取随机区组设计,进行相同施肥管理水平下的密度试验,探索洛薯8号获得最好经济效益的栽培密度.结果表明:群体随密度增加而增大,但个体转弱,导致营养生长与生殖生长不协调,植株的抗病性、抗逆性变差;随着密度加大(45 000～90 000株/hm2),单株结薯个数由4.8个减少到3.6个,单株结薯质量由684.71 g减小到410.41 g,平均薯块质量由142.64 g减小到114.00 g,大薯率由75.26％减小到38.92％,商品率逐渐变差,由90.64％下降到63.54％;产量随着栽培密度的增加呈现先增加后减少趋势,栽植密度为45 000株/hm2时产量最低,为30 811.80 kg/hm2,密度为67 500株/hm2时产量达到峰值,为38 426.10 kg/hm2,随后产量逐渐降低.密度67 500株/hm2与密度为60 000、75 000、82 500株/hm2之间产量差异不显著,而与其他密度(45 000、52 500、90 000株/hm2)处理间存在显著差异.从而得出洛薯8号在河南两季作区的适宜栽植密度为60 000～82 500株/hm2,最适宜密度为67 500株/hm2.  相似文献   

Chronic alcohol intake-induced epithelial-mesenchymal transition contri-butes to hepatic fibrogenesis in mice     

SUN Yu-sheng  LIN Bo  WANG Si-qian  LIU Yue  ZHANG You-jing  ZHENG Nai-rui  HUANG-FU Chao-shen 《园艺学报》2014,30(1):77-84

AIM：To evaluate the effect of chronic alcohol intake on the histopathological changes of the liver and to determine the contribution of epithelial-mesenchymal transition (EMT) to hepatic fibrogenesis. METHODS：Thirty male C57BL/6 mice were randomly divided into 3 groups as following: the mice in control group was given (ig) water; the mice in low-dose alcohol group (2.0 g·kg -1·d -1) and high-dose alcohol group (4.0 g·kg -1·d -1) were given (ig) alcohol for 5 months. Alcohol-induced histopathological changes of the liver or development of hepatic fibrosis were evaluated using the histological methods with HE and Masson trichrome staining. The apoptosis of the liver was detected by TUNEL fluorometric staining (counterstained with DAPI). The activity of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) was measured by an automated biochemical analyzer. The expression of fibroblast-specific protein 1 (FSP-1), α-smooth muscle actin (α-SMA) and E-cadherin in the hepatic tissues was detected by immunofluorescence examination. The protein levels of E-cadherin, α-SMA, FSP-1, transforming growth factor β 1 (TGF-β 1) and hypoxia-inducible factor 1α (HIF-1α) were analyzed by Western blotting. RESULTS：Compared with control, the activity of serum ALT and AST, and apoptotic index of liver tissues were increased in the mice treated with alcohol for 5 months. The histopathological changes of the livers in the mice of low-dose alcohol group included steatosis and mild liver fibrosis, while severe liver fibrosis was observed in the high-dose alcohol-treated mice. Chronic alcohol consumption induced the increase in malondialdehyde (MDA) level, and the decreases in the activity of superoxide dismutase (SOD) and catalase (CAT) in the livers. It also reduced E-cadherin expression and increased α-SMA expression. FSP-1 immunostaining and albumn immunostaining positive cells were co-localized in the hepatocytes of low-dose alcohol group, but only FSP-1 positive hepatocytes were observed in high-dose alcohol group. Chronic alcohol consumption decreased E-cadherin expression and increased α-SMA, FSP-1, TGF-β 1 and HIF-1α expression in a dose-dependent manner, but the HIF-1α expression was not altered between the 2 alcohol-treated groups. CONCLUSION：Chronic alcohol intake induces the progression of hepatic fibrosis. Some fibroblasts derive from hepatocytes in liver fibrosis via EMT. The underlying mechanism is associated with the changes of the redox state, and increased TGF-β 1 generation and HIF-1α expression.  相似文献