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1.
Teewara Phongnimitr Yuanyuan Liang Kanokwan Srirattana Kanchana Panyawai Nucharin Sripunya Chatchai Treetampinich Rangsun Parnpai 《Animal Science Journal》2013,84(11):719-725
In this study, the effects of the addition of L‐carnitine in in vitro maturation (IVM) medium for bovine oocytes on their nuclear maturation and cryopreservation were investigated; they were matured in IVM medium supplemented with 0.0, 0.3, 0.6 and 1.2 mg/mL of L‐carnitine (control, 0.3, 0.6 and 1.2 groups, respectively) and some of them were vitrified by Cryotop. Moreover, the effects of L‐carnitine during in vitro fertilization (IVF) and in vitro culture (IVC) on the developmental potential and quality of IVF embryos were also examined. A significantly higher maturation rate of oocytes was obtained for 0.3 and 0.6 mg/mL groups compared with the control (P < 0.05). The blastocyst formation rate in the 0.6 group was significantly improved, whereas the rate in the 1.2 group was significantly decreased when compared with the control group (P < 0.05). No significant difference was found in embryo development between the control and the L‐carnitine group after oocyte vitrification. Supplementation of IVF and IVC media with L‐carnitine had no effect on development to the blastocyst stage of IVM oocytes treated with 0.6 mg/mL L‐carnitine. In conclusion, the supplementation of L‐carnitine during IVM of bovine oocytes improved their nuclear maturation and subsequent embryo development after IVF, but when they were vitrified the improving effects were neutralized. 相似文献
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Ashit Kumar Paul Yuanyuan Liang Kanokwan Srirattana Takashi Nagai Rangsun Parnpai 《Animal Science Journal》2018,89(2):307-315
In the present study, we aimed to determine the applicability of a paper container for the vitrification of in vitro matured (IVM) bovine oocytes. In experiment 1, IVM oocytes were exposed to vitrification solution (20% dimethylsulfoxide (DMSO), 20% ethylene glycol (EG), and 5 mol/L sucrose), using a two‐step method, for 30 s; loaded onto either a paper container or Cryotop; and stored in liquid nitrogen. No significant difference (P < 0.05) in the survival and blastocyst formation rates after in vitro vitrification was observed between the paper container and Cryotop. In experiment 2, IVM oocytes were exposed to either a two‐ or three‐step vitrification solution. The three‐step vitrification solution was not significantly different from the two‐step solution in terms of oocyte survival, cleavage and blastocyst rates. In experiment 3, in vitro produced blastocysts were graded according to the manual of the International Embryo Transfer Society (grades 1 and 2) and vitrified using the two‐ and three‐step methods. For grade 2 blastocysts, the three‐step method showed significantly higher (P < 0.05) survival and hatched blastocyst rates than the two‐step method, whereas for grade 1 blastocysts, no significant difference was observed. In conclusion, the paper device and three‐step technique are suitable for oocytes and embryo vitrification. 相似文献
3.
Changwichit K Khorana N Suwanborirux K Waranuch N Limpeanchob N Wisuitiprot W Suphrom N Ingkaninan K 《Fitoterapia》2011,82(6):798-804
The ethanolic extract from stems of a Thai medicinal plant, Alstonia macrophylla Wall. ex G. Don (Apocynaceae) showed a significant inhibitory effect on acetylcholinesterase (AChE) determined by using Ellman assay. Four compounds i.e., a bisindole alkaloid, macralstonine (1), a new bisindole alkaloid, thungfaine (2), a secoiridoid glycoside, sweroside (3) and a new secoiridoid glycoside, naresuanoside (4) were isolated. Compound 4 showed moderate AChE and butyrylcholinesterase (BChE) inhibitory effects. Interestingly, compound 4 inhibited cell growth on human androgen-sensitive prostate cancer cell line (LNCaP) but no effect on viability of human foreskin fibroblast cells (HF). 相似文献
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Imsoonthornruksa S Lorthongpanich C Sangmalee A Srirattana K Laowtammathron C Tunwattana W Somsa W Ketudat-Cairns M Nagai T Parnpai R 《The Journal of reproduction and development》2011,57(3):385-392
The aim of this study was to investigate if reconstructed felid embryos obtained by intraspecies or intergeneric cloning can develop in vitro. Fibroblast cells (f) from a domestic cat (DCf), marbled cat (MCf) and bovine (Bf) were used as donor cells, and oocytes (o) from domestic cats (DCo) and bovine (Bo) were used as recipient cytoplasts. There were two intraspecies (donor cell + recipient cytoplast: DCf + DCo and Bf + Bo) and three intergeneric (MCf + DCo, DCf + Bo and MCf + Bo) cloning groups in the study. In Experiment 1, the effects of manipulation media, modified TCM-199 (199H) or Emcare holding medium (EHM), on in vitro development of DCf + DCo embryos were investigated. The blastocyst formation rate (BFR) of the embryos manipulated in EHM (33.3%) was higher (P<0.05) compared with those manipulated in 199H (18.1%). In Experiment 2, DCf + DCo and MCf + DCo embryos were cocultured with or without domestic cat oviductal epithelium cells. Irrespective of coculture, the same BFR was obtained for DCf + DCo embryos (44.4 vs. 38.0%), while MCf + DCo embryos could not develop beyond the morula stage. In experiment 3, although the development of MCf + DCo and DCf + Bo embryos was arrested at the morula stage, 8.6% of MCf + Bo embryos were able to develop to the blastocyst stage. These results demonstrated that EHM was superior to 199H as an embryo manipulation medium and that the DCo and Bo could support the early embryonic development of intergeneric cloned marbled cat embryos up to the morula stage. However, postimplantation development still needs to be investigated. 相似文献
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Kanokwan KAEWMUNGKUN Keasinee TONGMARK Sriprapai CHAKHONKAEN Numphet SANGARWUT Thiwawan WASINANON Natjaree PANYAWUT Khanittha DITTHAB Kannika SIKAEWTUNG QI Yong-bin Sukanya DAPHA Atikorn PANYA Natthaporn PHONSATTA Amorntip MUANGPROM 《农业科学学报》2023,22(3):679-690
Rice is the staple food for about half of the world’s population.Preferred by consumers,aromatic rice is a special type of rice with great commercial value.Cooking and eating qualities and aroma are the major grain qualities favored by most consumers.Currently,most of the available aromatic varieties have low yields and some undesirable agronomic traits.Thus,there is an urgent need to develop better aromatic rice varieties.This work aims to identify rice germplasm lines that have good grain qual... 相似文献
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Singasa Kanokwan Songserm Taweesak Lertwatcharasarakul Preeda Arunvipas Pipat 《Tropical animal health and production》2017,49(7):1523-1529
Tropical Animal Health and Production - Bovine coronavirus (BCoV) is involved mainly in enteric infections in cattle. This study reports the first molecular detection of BCoV in a diarrhea outbreak... 相似文献
7.
Latiporn Udomsuk Waranya Chatuphonprasert Orawan Monthakantirat Yaowared Churikhit Kanokwan Jarukamjorn 《Fitoterapia》2012
Miroestrol (MR) is a highly active phytoestrogen isolated from tuberous root of Pueraria candollei var. mirifica (PM). Modulatory effects of PM and MR on osteoprotegerin (OPG) and receptor activator of nuclear factor kappa B ligand (RANKL) mRNAs which are bone-specific genes were investigated in ovariectomized female ICR mice. After ovariectomy, expression of OPG mRNA was suppressed but that of RANKL was induced. Estradiol benzoate (E2) recovered OPG expression to the level comparable to the sham while that of RANKL was suppressed in ovariectomized mice. PM crude extract (PME) significantly down-regulated the expression of RANKL mRNA with no change in the OPG level whereas MR elevated the expression of OPG mRNA with lowering level of RANKL mRNA, resulting in the increased OPG/RANKL ratio, and consequently lead to lowering progression of osteoporosis at molecular level. These findings revealed potential of PME and MR on bone loss prevention via increasing the ratio of OPG to RANKL (osteoformation/osteoresorption) in liver of ovariectomized mice. Therefore, using PME and MR as alternative hormone replacement therapy of E2 might be beneficial recommended due to advantageous on regulation of osteoporosis related genes. 相似文献
8.
Impacts of berberine, a major isoquinoline alkaloid in herbal plants, on beta-naphthoflavone (BNF)-induced CYP1A expression were determined both in primary mouse hepatocytes and in vivo. Berberine concentration-dependently suppressed BNF-induced CYP1A expression in mouse hepatocyte and it significantly down-regulated BNF-induced CYP1A in mouse liver via suppression of mRNA and protein expression, and decreases of EROD and MROD activities. Moreover, berberine showed significant potential on suppression of BNF-induced lipid peroxidation in mouse hepatic microsome. Therefore, using berberine as a health supplement or an alternative medication might provide extra-benefit due to its inhibitory regulation on CYP1A expression and anti-lipid peroxidation activity. 相似文献
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Kanokwan SRIRATTANA Kazutsugu MATSUKAWA Satoshi AKAGI Mariko TASAI Takahiro TAGAMI Keijiro NIRASAWA Takashi NAGAI Yukio KANAI Rangsun PARNPAI Kumiko TAKEDA 《Animal Science Journal》2011,82(2):236-243
Although interspecies/intergeneric somatic cell nuclear transfer (iSCNT) has been proposed as a tool to produce offspring of endangered species, conflict between donor nucleus and recipient cytoplasm in iSCNT embryos has been identified as an impediment to implementation for agricultural production. To investigate the nuclear–mitochondrial interactions on the developmental potential of iSCNT embryos, we analyzed the mtDNA copy numbers in iSCNT embryos reconstructed with water buffalo (swamp type) fibroblasts and bovine enucleated oocytes (buffalo iSCNT). As controls, SCNT embryos were derived from bovine fibroblasts (bovine SCNT). Buffalo iSCNT and bovine SCNT embryos showed similar rates of cleavage and development to the 8‐cell stage (P > 0.05). However, buffalo iSCNT embryos did not develop beyond the 16‐cell stage. Both bovine and buffalo mtDNA content in buffalo iSCNT embryos was stable throughout the nuclear transfer process, and arrested at the 8‐ to 16‐cell stage (P > 0.05). In bovine SCNT embryos that developed to the blastocyst stage, mtDNA copy number was increased (P < 0.05). In conclusion, both the donor cell and recipient cytoplast mtDNAs of buffalo iSCNT embryos were identified and maintained through the iSCNT process until the 8–16‐cell stage. In addition, the copy number of mtDNA per embryo was a useful monitor to investigate nuclear–mitochondrial interactions. 相似文献