Immunohistochemical and qPCR determination of the expression and serum level of anti‐Müllerian hormone in pre‐pubertal,intact and ovarian remnant syndrome detected bitches     

Kubra Karakas Alkan  Ahmet Ceylan  Hasan Alkan  Dogukan Ozen  Alev Gurol Bayraktaroglu  Mustafa Kaymaz 《Reproduction in domestic animals》2019,54(7):979-986

The aim of this study was to determine the serum concentrations, ovarian presence and expression of anti‐Müllerian hormone (AMH) in pre‐pubertal, bitches with signs of ovarian remnant syndrome (ORS) and intact bitches. In addition, we aimed to verify the suitability of serum AMH concentrations for diagnostic purposes in sterilized bitches and/or in suspected cases of ORS in the field of veterinary medicine. For this purpose, 36 healthy female dogs divided into six groups: proestrus, oestrus, dioestrus, anoestrus, pre‐pubertal and ORS. Serum AMH concentrations were determined by electrochemiluminescence immunoassay, and ovarian presence and distribution of AMH was confirmed by immunohistochemical and qPCR techniques. According to the results of qPCR, while the expression values of AMH were at the highest concentrations in the proestrus and oestrus, there was a statistically significant decrease in these values at the later stages of the cycle (p < 0.05). According to hormone analysis, the serum AMH values of the ORS group had decreased significantly compared with the proestrus and oestrus (p < 0.05). Although serum AMH levels of ORS group were increased compared with anestrus and pre‐pubertal groups, this increase was statistically non‐significant (p > 0.05). Immunohistochemically, AMH expression was first observed in the granulosa cells of primordial follicles in folliculogenesis. Expression values were the highest in the proestrous and oestrus groups, but values from bitches in later stages of the cycle were statistically significant decrease in comparison with these groups (p < 0.05). As a result, AMH concentration and expression were found to be higher in proestrus and oestrus than in other periods (p < 0.05). In addition, the measurable level of AMH concentration in bitches with ORS is an indication that it can be used in the diagnosis of ORS.  相似文献   

The Effect of Total Anthocyanins Extracted From Sweet Cherry Cultivars on Carbonic Anhydrases and Antioxidant Activity     

Sonmez  Fatih  Gunesli  Zuhal  Demir  Taki  Cıkrıkcı  Kubra  Ergun  Adem  Gencer  Nahit  Arslan  Oktay 《Erwerbs-Obstbau》2022,64(2):145-153

Total anthocyanins were extracted from eleven sweet cherry cultivars grown in Sakarya, Turkey. In vitro inhibition effects of extracted total anthocyanins on purified carbonic anhydrase I and II were investigated by using CO₂ as a substrate. The results showed that all extracted total anthocyanins inhibited the hCA I and hCA II enzyme activities. Among all cultivars, ‘Merton Premier’ was found to be the most active one against both hCA I (IC₅₀?=?0.83?µg/mL) and hCA II (IC₅₀?=?1.16?µg/mL). Additionally, the DPPH activities of the extracts were investigated as antioxidant properties. The results exhibited that ‘Churchill’ and ‘Merton Premier’ have high antioxidant activity with 74.102% and 73.503% DPPH activity, respectively.

  相似文献   

A functional bread produced by supplementing wheat flour with high β-glucan hull-less barley flour     

Hamit Koksel  Buket Cetiner  Kubra Ozkan  Zeynep Hazal Tekin-Cakmak  Osman Sagdic  Francesco Sestili  Abderrazek Jilal 《Cereal Chemistry》2024,101(3):630-640

  相似文献   

Robust molecular detection of the new Tomato brown rugose fruit virus in infected tomato and pepper plants from Turkey          下载免费PDF全文

Hakan FIDAN  Pelin SARIKAYA  Kubra YILDIZ  Bengi TOPKAYA  Gozde ERKIS  Ozer CALIS 《农业科学学报》2021,20(8):2170-2179

  相似文献