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1.
Takuya Ban Mihoko Kugishima Tsuneo Ogata Shuji Shiozaki Shosaku Horiuchi Hisafumi Ueda 《Scientia Horticulturae》2007
Effect of ethephon (2-chloroethylphosphonic acid) application on rabbiteye blueberry fruit quality during the growth period was investigated. Ethephon treatment stimulated the decrement of titratable acidity, anthocyanin accumulation and fruit softening 4 days after treatment and the promoting effects continued through the investigation period. The ripening promotion effect of ethephon on total soluble solids content was observed only 8 days after treatment. Ethephon treatment did not affect the fruit enlargement during the investigation period. From these results, it is concluded that ethephon application for rabbiteye blueberry promote the fruit ripening, but the stimulatory effects of ethephon on fruit ripening were different in degree on each ripening characters. 相似文献
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Cleridy E. Lennert‐Cody Shelley C. Clarke Alexandre Aires‐da‐Silva Mark N. Maunder Peter J. S. Franks Marlon Romn Arthur J. Miller Mihoko Minami 《Fisheries Oceanography》2019,28(1):43-53
Recent large fluctuations in an index of relative abundance for the silky shark in the eastern Pacific Ocean have called into question its reliability as a population indicator for management. To investigate whether these fluctuations were driven by environmental forcing rather than true changes in abundance, a Pacific‐wide approach was taken. Data collected by observers aboard purse‐seine vessels fishing in the equatorial Pacific were used to compute standardized trends in relative abundance by region, and where possible, by shark size category as a proxy for life stage. These indices were compared to the Pacific Decadal Oscillation (PDO), an index of Pacific Ocean climate variability. Correlation between silky indices and the PDO was found to differ by region and size category. The highest correlations by shark size category were for small (<90 cm total length [TL]) and medium (90–150 cm TL) sharks from the western region of the equatorial eastern Pacific (EP) and from the equatorial western Pacific. This correlation disappeared in the inshore EP. Throughout, correlations with the PDO were generally lower for large silky sharks (>150 cm TL). These results are suggestive of changes in the small and medium silky indices being driven by movement of juvenile silky sharks across the Pacific as the eastern edge of the Indo‐Pacific Warm Pool shifts location with ENSO events. Lower correlation of the PDO with large shark indices may indicate that those indices were less influenced by environmental forcing and therefore potentially less biased with respect to monitoring population trends. 相似文献
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Mihoko FUSHII Rie YAMADA Jibak LEE Takashi MIYANO 《The Journal of reproduction and development》2021,67(5):300
Transzonal projections (TZPs) that maintain bidirectional communication between oocytes and granulosa cells or cumulus cells are important structures for oocyte growth. However, whether TZPs develop between TZP-free oocytes and granulosa cells, and whether reestablished TZPs support oocyte growth, is unknown. We first examined changes in TZPs after denudation of bovine oocytes collected from early antral follicles (0.5–0.7 mm). Twenty-four hours after denudation, almost all the TZPs disappeared. We also examined the reestablishment of TZPs by coculturing TZP-free denuded oocytes (DOs) with mural granulosa cells (MGCs) collected from early antral follicles. In addition, to confirm if the reestablished TZPs were functional, the reconstructed complexes (DO+MGCs) were subjected to in vitro growth culture and found that the MGCs adhered to TZP-free DOs and TZPs were reestablished. During in vitro growth culture, DO+MGCs developed and formed antrum-like structures. After culture, the number of TZPs in DO+MGCs increased, and the oocytes grew fully and acquired meiotic competence. These results suggest that reestablished TZPs are able to support oocyte growth. 相似文献
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Anagawa A Okazaki Y Murakami Y Tsubota K Ono M Matsumoto M Nakatsuji S Oishi Y 《Journal of toxicologic pathology》2009,22(3):205-208
A firm, tan, well-circumscribed mass that measured 25 × 30 × 35 mm was observed in the thoracic cavity of a 53-week-old male Crl:CD(SD) IGS rat. Histologically, the mass was encapsulated by fibrous tissue and contained fibrovascular septae. Tumor cells were compactly arranged, and most were oval to polygonal in shape with multivacuolated cytoplasm and a centrally located nucleus. In some parts of the tumor, marked cellular atypia and frequent mitoses were evident. Vacuoles in cytoplasm were positive for oil red O. The tumor cells were characterized ultrastructurally by abundant, round to oval mitochondria with transverse closely-packed cristae. Tumor cells were immunohistochemically positive for uncoupling protein 1 (UCP-1). Several thrombi and hemorrhagic or necrotic foci were also observed within the tumor mass. Vascular invasion of the tumor capsule was observed; however, invasion of surrounding tissues or metastases were not observed. Based on the pathology findings, this case was diagnosed as a malignant hibernoma. 相似文献
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Several successful in vitro culture experiments have used oocyte-cumulus cell-mural granulosa cell complexes (OCGCs) from early antral follicles (0.5–0.7 mm) for the growth of bovine oocytes. However, in studies related to in vitro oocyte maturation and in vitro embryo production, oocyte-cumulus cell complexes (OCCs) that have no mural granulosa cells have been widely used instead of OCGCs. The purpose of this study was to determine whether cumulus cells alone support oocyte growth. First, OCCs and OCGCs were cultured in vitro for 14 days to compare the integrity of the complexes as well as antrum formation. After 14 days, the diameter and meiotic competence of oocytes in OCCs and OCGCs were examined. Oocytes in OCCs grew fully and acquired meiotic competence similar to OCGCs, whereas antrum formation occurred later in OCCs as compared to OCGCs. Subsequently, the effects of follicle stimulating hormone (FSH) on in vitro growth of OCCs were examined for 14 days. When FSH was added to the culture medium, OCCs formed antrum-like structures one day earlier than those cultured without FSH. Oocytes cultured with 1 mIU/ml FSH grew fully and acquired meiotic competence. In contrast, when oocytes were cultured in media containing high concentrations of FSH, some of the OCCs collapsed and the number of degenerated oocytes increased. In conclusion, bovine oocytes in OCCs grow and acquire meiotic competence similar to OCGCs and, 1 mIU/ml FSH supports the development of OCCs and oocyte growth as observed in our culture system. 相似文献
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Mizuhiko Nishida Mihoko Moriizumi Kazunari Tsuchiya 《Soil Science and Plant Nutrition》2005,51(4):577-581
The N recovery from 15 N-labeled swine manure compost and rice bran with or without simultaneous application of unlabeled cattle manure compost was examined in a paddy field with direct-seeded rice during a 1-year period (1 crop season). In all the 15 N-labeled materials including (15 NH4 )2 SO4 , the processes of N recovery from the 15 N materials by rice plants were different between the plots with and without application of cattle manure compost. At the tillering stage, the N recovery rates from the 15 N materials in the plots with application of cattle manure compost were significantly lower than those in the plots without application of cattle manure compost. These recovery rates, however, became close and no significant differences were observed at the maturity stage. Thus, simultaneous application of cattle manure compost could impede the N recovery from swine manure compost, rice bran as well as (NH4 )2 SO4 . 相似文献
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Jones S Zhang X Parsons DW Lin JC Leary RJ Angenendt P Mankoo P Carter H Kamiyama H Jimeno A Hong SM Fu B Lin MT Calhoun ES Kamiyama M Walter K Nikolskaya T Nikolsky Y Hartigan J Smith DR Hidalgo M Leach SD Klein AP Jaffee EM Goggins M Maitra A Iacobuzio-Donahue C Eshleman JR Kern SE Hruban RH Karchin R Papadopoulos N Parmigiani G Vogelstein B Velculescu VE Kinzler KW 《Science (New York, N.Y.)》2008,321(5897):1801-1806