Field evaluation of a coproantigen enzyme-linked immunosorbent assay for diagnosis of canine echinococcosis in a rural Andean village in Peru     

Lopera L  Moro PL  Chavez A  Montes G  Gonzales A  Gilman RH 《Veterinary parasitology》2003,117(1-2):37-42

One hundred and six dogs (61 males and 45 females) were examined for Echinococcus granulosus infection in a farming cooperative in the central highlands of Peru during November 1998. Canine echinococcosis was diagnosed using direct microscopic examinations of purged feces following arecoline purging and a coproantigen-detection enzyme-linked immunosorbent assay (ELISA) for E. granulosus. Mean age was 2 years with a range of 3 months to 9 years. The overall prevalence of canine echinococcosis using the ELISA test was 79% (84/106). Seventy-four dogs were successfully purged with arecoline. The frequency of canine echinococcosis was 82 (61/74) and 34% (25/74) by the coproantigen ELISA test and arecoline purging, respectively. The sensitivity and specificity of the coproantigen ELISA test was 88 and 95%, respectively. We found this assay to be especially advantageous in remote geographical areas. In future control programs against echinococcosis in Peru and other areas where E. granulosus is endemic the coproantigen ELISA should be used for the surveillance of the dog population.  相似文献   

Morphology and amplitude values of the electrocardiogram of Spanish-bred horses of different ages in the Dubois leads system     

Ayala I  Gutierrez-Panizo C  Benedito JL  Prieto F  Montes A 《Veterinary research》2000,31(3):347-354

The aim of this work was to record the modifications of the form and amplitude of the electrocardiographic tracings during growth using the Dubois system and to study its sensibility to these physiological changes. This work was carried out on 179 healthy, young and adult Spanish-bred horses (98 females and 81 males). One electrocardiogram (ECG) was obtained from each horse using the Dubois leads system. The bifid shape deflection of the P wave was generally more frequent than the simple one in animals at 4 months of age or older. Amplitudes of the P2 component and of the simple positive wave presented significant differences (p < 0.01) between the age groups studied: animals up to 3 months in age and older than 2 years of age (highest amplitudes), and the rest of the animals (lowest amplitudes). Significant variations (p < 0.05) were found for the QS amplitude between animals of 1 month of age, and all the other groups. The multiple range analysis did not define a clear distribution of age groups for the amplitude of the T wave. It may therefore be concluded, that in the Spanish-bred horse up to 1 month of age, the form and amplitude of the QRS complex in the Dubois leads system differ from those of older animals. Thus, this study shows the sensibility of the Dubois leads system for detecting the electrocardiographic changes related to the growing process in the Spanish-bred horse. In addition, it provides standard values of electrocardiographic parameters in the Spanish-bred horse at different stages of growth.  相似文献   

Sodicity and salinity in a Brazilian Oxisol cultivated with sugarcane irrigated with wastewater   总被引：2，自引：0，他引：2  

Rafael Marques Pereira Leal  Uwe Herpin  Lilian Pittol Firme  Célia Regina Montes  Adolpho José Melfi 《Agricultural Water Management》2009,96(2):307-316

Changes in soil sodicity-salinity parameters are one of the most characteristic alterations after treated sewage effluent (TSE) irrigation in agro-systems. Considering the importance of these parameters for agricultural management, as well as the economical value of sugarcane for Brazil, the present study aimed at evaluating effects on soil sodicity and salinity under tropical conditions over 16 months of TSE irrigation in a sugarcane plantation at Lins, São Paulo State, Brazil. Soil samplings were carried out in February 2005 (before planting), December 2005 (after 8 months of TSE irrigation) and September 2006 (after 16 months of TSE irrigation) following a complete block design with four treatments and four replicates. Treatments consisted of: (i) control, without TSE irrigation; (ii) T100, T150 and T200, with TSE irrigation supplying 100% (0% surplus, total of 2524 mm), 150% (50% surplus, total of 3832 mm) and 200% (100% surplus, total of 5092 mm) of crop water demand, respectively. Compared to initial soil conditions, at the end of the experiment increases of exchangeable sodium (from 2.4 to 5.9 mmol_c kg⁻¹), exchangeable sodium percentage (ESP) (from 8 to 18%), soluble Na (from 1.4 to 4.7 mmol L⁻¹) and sodium adsorption ratio (SAR) of soil solution (from 3.6 to 12.6 (mmol L⁻¹)^0.5) were found in the soil profile (0-100 cm) as an average for the irrigated plots due to high SAR of TSE. Associated with the increments were mostly significant increases in clay dispersion rates at depths 0-10, 10-20 and 20-40 cm. Electrical conductivity (EC) of soil solution increased during the TSE irrigation period whereas at the end of the experiment, after short term discontinuation of irrigation and harvest, EC in the topsoil (0-10 and 10-20 cm) decreased compared to the previous samplings. Moreover, despite increasing sodicity over time mainly insignificant differences within the different irrigated treatments were found in December 2005 and September 2006. This suggests that independent of varying irrigation amounts the increasing soil sodicity over time were rather caused by the continuous use of TSE than by its quantity applied. Moreover, also plant productivity showed no significant differences within the TSE irrigated plots. The study indicates that monitoring as well as remediation of soil after TSE irrigation is required for a sustainable TSE use in order to maintain agricultural quality parameters.  相似文献   

Management effects on carbon stocks and fluxes across the Orinoco savannas     

Jose J. San Jos  Rubn A. Montes 《Forest Ecology and Management》2001,150(3):293-311

Across the physiognomic types of the Orinoco llanos, periodic inventories and changes in land-use between 1982–1992 are estimated. Results indicate that the area under pastures and forest plantations is increased by 0.005337×10⁶ km², whilts reducing the area of croplands by 0.000119×10⁶ km². This is a net increase of 0.005218×10⁶ km². The gross carbon release is 174.66 Tg C per year to the atmosphere and transferring from cultivated and native vegetation to wood products (1.62 Tg C per year) and slash (1.18 Tg C per year). The processes of land preparation contribute 1.40 Tg C per year to the atmosphere. From the tree savannas, woodlands and forests 0.73 Tg C per year are estimated to have been transferred to the soil following clearance and burning over this period, and 1.05 Tg C per year from herbaceous savannas when were buried and decomposed at 0.84 Tg C per year. The estimate of carbon balance here by inventories and changes in land-use approach indicates that the Orinoco llanos is a sink of −17.53 Tg C per year. The carbon turnover time in the Orinoco system is 68 years, which provides a limited route for carbon sequestration. The calculated potential of the Orinoco llanos for storing carbon is 8300 Tg C. Ecological options to achieve this potential value are addressed. However, nutrient deficiency and seasonal water supply are serious drawbacks to take into account for increasing carbon accretion. These results are particular for the Orinoco llanos, even though described processes could be similar to world-wide savannas, where a gradient of carbon heterogeneity exists.  相似文献   

Effects of two calculolytic diets on parameters of feline mineral metabolism          下载免费PDF全文

C. Pineda  E. Aguilera‐Tejero  A. I. Raya  A. Montes de Oca  M. Rodriguez  I. Lopez 《The Journal of small animal practice》2015,56(8):499-504

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Is this the end? Dynamics of a relict stand from pervasively deforested ancient Iberian pine forests     

G. Gea-Izquierdo  F. Montes  R. G. Gavilán  I. Cañellas  A. Rubio 《European Journal of Forest Research》2015,134(3):525-536

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Stem mapping and estimating standing volume from stereoscopic hemispherical images     

Cristina Rodríguez-García  Fernando Montes  Federico Ruiz  Isabel Cañellas  Pilar Pita 《European Journal of Forest Research》2014,133(5):895-904

Sustainable forest management requires knowledge of forest structure and dynamics as well as an estimation of growing stock. The forest inventory provides the data for estimating stand variables. The measurement device MU2005-01738, patented by the Center for Forest Research (INIA-CIFOR), provides stereoscopic hemispherical images which can allow the 3D restoration of the stand around the sampling point. The aim of this study is to develop a methodology for forest stand mapping as well as tree diameter and height measurement along with volume estimation from the stereoscopic hemispherical images provided by the MU2005-01738. Using the MU2005-01738, Eucalyptus globulus Labill. plantations were sampled. Distance, diameter, height and volume were derived from the stereoscopic hemispherical images of 30 trees located at distances ranging from 0 to 15 m from the device. These variables were then compared with field measurements and the estimation errors analyzed. The (root-mean-squared error) RMSE was 0.23 m (8.95 %) for tree position and 1.51 cm (10.43 %) for diameter at breast height measurement at distances of less than 8 m. In the case of stem height and individual tree volume estimation, the RMSE was 2.59 m (23.05 %) and 0.025 m³ (17.94 %), respectively. The analysis of measurement errors indicated that the measurement precision decreases beyond 8 m from the device as well as for directions close to the baseline (the line between the optical centers of the two images), whereas the precision was highest for directions near to the line which is perpendicular to the base line. Future research should focus on improving measurement accuracy and possible applications in the field of forestry of the techniques presented in this study.  相似文献   

Quality assessment of rapeseed accessions by means of near-infrared spectroscopy on combine harvesters     

J. M. Montes    C. Paul    A. E. Melchinger 《Plant Breeding》2007,126(3):329-330

Optimization of data collection processes in plant breeding programmes is of the highest importance for plant breeders. In rapeseed-breeding, data collection and selection must be carried out in a very restricted period of time because of the short time span between harvesting and sowing. Near-infrared spectroscopy on combine harvesters (NOCH) improves the data collection processes in breeding programmes of grain crops such as maize, and it may also improve the data collection processes in breeding programmes of rapeseed. The objective of this study was to assess the potential of NOCH for the determination of dry matter (DM), crude protein (CP), oil and glucosinolate (GSL) contents in rapeseed. A plot combine harvester equipped with a near-infrared diode-array spectrometer was used. Reference values for DM content were determined by the oven method. Reference values for the quality traits were established by laboratory near-infrared spectroscopy. NOCH showed a high potential for the determination of DM, CP, oil, and GSL contents. Use of NOCH in breeding may increase the efficiency of data collection processes and might dramatically accelerate the development of rapeseed cultivars.  相似文献   

Growth and Nutritional Disorders of Eggplant Cultivated in Nutrients Solutions with Suppressed Macronutrients     

Rilner Alves Flores  Bernardo Melo Montes Nogueira Borges  Hilário Júnior Almeida  Renato De Mello Prado 《Journal of plant nutrition》2015,38(7):1097-1109

The objective was to evaluate the effects of omitting macronutrients in the nutrients solution on growth characteristics and nutritional status of eggplants. The treatments were complete nutrients solution and solutions with nutrient omission: nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), and sulfur (S). The experiment was carried out under greenhouse conditions with three replicates in a completely random design. Plant height, number of leaves per plant, leaf area, relative chlorophyll index, photosynthesis rate, stomatal conductance, dry matter, concentration levels of macronutrients in plant aerial part and root system, and nutritional disorders were evaluated. Omitting elements interfered in the concentration of elements in the various plant tissues and this had as consequences limited vegetative growth, reduced dry matter and led to the development of the typical deficiency symptoms of each element. Although potassium was the most demanded of all elements, nitrogen and calcium were the most growth limiting ones.  相似文献   

Dermatophytosis due to Microsporum canis in a rhesus monkey   总被引：1，自引：0，他引：1  

H J Baker  L G Bradford  L F Montes 《Journal of the American Veterinary Medical Association》1971,159(11):1607-1611

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