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1.
Whereas the rise in temperature during the past 30–40 years has already had clear impacts on the phenology of fruit trees and pathogenic insects, there is a lack of such correlations for fungal pathogens. An examination of fruit rots indicates that pathogenic fungi react differentially to climate change due to their complex infection biology. The appearance of the black rot fungus Diplodia seriata in Northwestern Europe is best explained by rising temperatures during the vegetation period. An increase in fruit rot caused by Nectria galligena is anticipated when milder and more humid winter months favour canker formation on twigs and branches, thereby increasing inoculum for fruit infections. An increasing importance of Neofabraea alba and Glomerella cingulata and/or G.??acutata as storage rots of apples in Northern Germany cannot be safely correlated with the climate change at present. Research on fungi is currently being expanded at the OVB Jork in order to ensure a faster identification of new pathogens and a more thorough investigation of relevant features of their infection biology.  相似文献   
2.
Intensive vegetable production in greenhouses has rapidly expanded in China since the 1990s and increased to 1.3 million ha of farmland by 2016, which is the highest in the world. We conducted an 11‐year greenhouse vegetable production experiment from 2002 to 2013 to observe soil organic carbon (SOC) dynamics under three management systems, i.e., conventional (CON), integrated (ING), and intensive organic (ORG) farming. Soil samples (0–20 and 20–40 cm depth) were collected in 2002 and 2013 and separated into four particle‐size fractions, i.e., coarse sand (> 250 µm), fine sand (250–53 µm), silt (53–2 µm), and clay (< 2 µm). The SOC contents and δ13C values of the whole soil and the four particle‐size fractions were analyzed. After 11 years of vegetable farming, ORG and ING significantly increased SOC stocks (0–20 cm) by 4008 ± 36.6 and 2880 ± 365 kg C ha?1 y?1, respectively, 8.1‐ and 5.8‐times that of CON (494 ± 42.6 kg C ha?1 y?1). The SOC stock increase in ORG at 20–40 cm depth was 245 ± 66.4 kg C ha?1 y?1, significantly higher than in ING (66 ± 13.4 kg C ha?1 y?1) and CON (109 ± 44.8 kg C ha?1 y?1). Analyses of 13C revealed a significant increase in newly produced SOC in both soil layers in ORG. However, the carbon conversion efficiency (CE: increased organic carbon in soil divided by organic carbon input) was lower in ORG (14.4%–21.7%) than in ING (18.2%–27.4%). Among the four particle‐sizes in the 0–20 cm layer, the silt fraction exhibited the largest proportion of increase in SOC content (57.8% and 55.4% of the SOC increase in ORG and ING, respectively). A similar trend was detected in the 20–40 cm soil layer. Over all, intensive organic (ORG) vegetable production increases soil organic carbon but with a lower carbon conversion efficiency than integrated (ING) management.  相似文献   
3.
Journal of Plant Diseases and Protection - Comparative studies of different isolates of Neonectria ditissima obtained from canker lesions and rotten fruit showed that both five-septate macroconidia...  相似文献   
4.
In double rice-cropping system in China, zero tillage in late-season rice with straw return from early season rice is an emerging technology for saving inputs, shortening the lag time between rice crops, avoiding straw burning, and conserving natural resources. The objective of this 2-year field study was to determine the effects of tillage and straw return on N uptake, grain yield, and N use efficiency of late-season rice. Treatments were arranged in a split-plot design with four combinations of tillage and straw return as main plots and three N management practices as subplots. Tillage was either conventional soil puddling or zero tillage with newly harvested crop residue from early season rice either removed or placed on the soil surface without incorporation. The N treatments were zero-N control, site-specific N management (SSNM), and farmers’ N-fertilizer practice (FFP). Straw return regardless of tillage or N management did not reduce rice yield. In the second year, straw return significantly increased grain yield in the zero-N control. Chlorophyll meter readings at heading and total N uptake at maturity were higher with straw return in the zero-N control, suggesting that straw provides nutrients to rice in the late growing period. Zero tillage did not reduce N uptake, grain yield, and N use efficiency compared with conventional tillage. Despite large differences in timing and rate of N application between FFP and SSNM, these two N treatments resulted in comparable N uptake and grain yield of late-season rice regardless of tillage and straw return. These results suggest that zero tillage after early rice with straw return could replace conventional tillage for late rice in the double rice-cropping system in China.  相似文献   
5.
A total of 55 non-surgical collections, 7 days after spontaneous heat, was made in 19 heifers. Recovery rate was 57% (17/30) from 10 repeat breeder heifers and 72% (18/25) from 9 virgin heifers, this difference was not significant. Fertilization rate in both groups was 94%. The eggs were always recovered in the first 100 ml of flushing medium. Mean lengths of oestrous cycle after non-surgical collection were, respectively, 16.9 and 18.1 days in repeat breeder and virgin heifers. This difference was not significant. However, the cycle in which the first collection was made was significantly shorter (P<.005) than the cycle immediately before the collections. Only 61% of possible eggs were non-surgically collected from 4 superovulated repeat breeder cows and no further eggs were found after slaughter, one to two hours later.  相似文献   
6.
A method for preparation of chromosomes from bovine zygotes and blastocysts   总被引:2,自引:0,他引:2  
A simple technique for making chromosome preparations from zygotes and early blastocysts is described. The morphological features of blastocysts and total number of cells greatly influence the quality of the preparation.  相似文献   
7.
The cause of death in a postsmolt, Atlantic salmon population with elevated levels of mortalities was investigated. Diagnosis of a rickettsia-like organism was based on gross pathology, histopathology, differential staining, electron microscopy and fluorescent antibody tests. The course of the infection and response to treatment are discussed. This is the first reported occurrence of salmon rickettsias in the Atlantic coast of North or South America.  相似文献   
8.
Studies were carried out to determine the cause of death in a prematurely born Thoroughbred foal that died 24 hours after birth. Necropsy revealed gross lesions suggestive of septicemia. A commercial Leptospira polymerase chain reaction (PCR) assay designed to specifically amplify the hemolysis-associated protein 1 (hap1) gene present only in pathogenic Leptospira strains detected the presence of Leptospira DNA in various tissues of the foal. Histologic examination of lung, liver, kidney, and myocardium revealed numerous spirochetes in Warthin-Starry-stained tissue sections. Results of PCR analysis and histologic examination suggested a leptospiral infection in the newborn foal. At the moment of death, the infection coexisted with a streptococcal-associated aspiration bronchopneumonia and postpartum septicemia. These findings indicate that the PCR assay based on the amplification of the hap1 gene represents a useful tool for specific detection of pathogenic leptospira in field samples taken from horses.  相似文献   
9.
The development and use of modern techniques, such as intracytoplasmic sperm injection (ICSI), gene knockout and sperm fluorescence in situ hybridization with chromosome- specific probes, have significantly increased our knowledge about sperm defects. We describe a new oligoasthenoteratozoospermic defect in a bull. Because of its morphological characteristics the defect was named the multinuclear-multiflagellar sperm defect. All spermatozoa in the ejaculate were abnormal. Many of the spermatozoa had multiple nuclei and multiple sperm tails. All spermatozoa lacked an acrosome, and only seldom did spermatozoa have a mitochondrial helix in the midpiece area. Meiosis and spermiogenesis were severely affected in this otherwise phenotypically normal bull. The sperm defects resembled the phenotype of a targeted gene knockout Hrb(-/-) (HIV-1 Rev-binding/interacting protein) mutant mouse strain, which is expressed as sterility in males, while females remain fertile. Since the father of this bull has been extensively used in at least three countries the defective gene has possibly become widespread in the red and white breeds (Ayrshire, Swedish Red and White, Norwegian Red) in the Nordic countries. However, it is not proved that the father of this bull is a carrier of this defect.  相似文献   
10.
A study was conducted to investigate the possibilities to develop models for predicting the relative silage dry matter intake (SDMI) in dairy cows utilising information on chemical composition and in vitro gas production (GP) kinetics of silages. In five experiments, each with an average of 38 lactating dairy cows, SDMI was recorded for 15 grass silages made from primary growth and regrowth swards of timothy (Phleum pratense L.). The silages were characterised by chemical analysis and by utilising an automated in vitro GP recording technique with end point measurements of substrate residues. The silage samples were analysed both as dried and wet samples to evaluate the effects of sample preparation techniques on GP kinetics and their relations to SDMI. Relationships between feed variables and SDMI were investigated utilising simple linear and multiple regression. The wet silage samples had higher cumulative GP and different GP curves compared to the dried samples. The linear relationships between, GP variables, harvest number (first or second cut) of the grass, chemical characteristics of the silages and the relative SDMI show that the GP technique is a powerful tool to detect silage quality. By using the parameters from the dried samples the multiple regression analysis resulted in a relationship, relative SDMI (kg per 100 kg live weight (LW)) per day = 0.071 + 0.0029 × NDFD − 0.266 × C (R2 = 0.82, S.D. = 0.06). NDFD is the degradability of the neutral detergent fibre (NDF) (g/kg NDF) and C is the variable that regulates the switching characteristics of the GP profiles. By using the wet silage samples the multiple relationship did not include any GP variable; relative SDMI (kg per 100 kg LW per day) = 1.86 − 0.008 × acetic acid (g/kg DM) + 0.024 × ethanol (g/kg DM) (R2 = 0.62, S.D. = 0.08). The results from the regression analysis and the experience of the laborious sample preparation technique for wet samples, give the conclusion that dried silage samples are recommended for determining feed characteristics using the GP technique in intake studies.  相似文献   
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