Epidemiology and diagnosis of Mycobacterium tuberculosis in captive Asian elephants (Elephas maximus).     

S K Mikota  L Peddie  J Peddie  R Isaza  F Dunker  G West  W Lindsay  R S Larsen  M D Salman  D Chatterjee  J Payeur  D Whipple  C Thoen  D S Davis  C Sedgwick  R J Montali  M Ziccardi  J Maslow 《Journal of zoo and wildlife medicine》2001,32(1):1-16

The deaths of two Asian elephants (Elephas maximus) in August 1996 led the United States Department of Agriculture to require the testing and treatment of elephants for tuberculosis. From August 1996 to September 1999. Mycobacterium tuberculosis infection was confirmed by culture in 12 of 118 elephants in six herds. Eight diagnoses were made antemortem on the basis of isolation of M. tuberculosis by culture of trunk wash samples; the remainder (including the initial two) were diagnosed postmortem. We present the case histories, epidemiologic characteristics, diagnostic test results, and therapeutic plans from these six herds. The intradermal tuberculin test, enzyme-linked immunosorbent assay serology, the blood tuberculosis test, and nucleic acid amplification and culture are compared as methods to diagnose M. tuberculosis infection in elephants.  相似文献   

High prevalence and antimicrobial resistance of mecA Staphylococcus aureus in dairy cattle,sheep, and goat bulk tank milk in Jordan     

Obaidat  Mohammad M.  Bani Salman  Alaa E.  Roess  Amira A. 《Tropical animal health and production》2018,50(2):405-412

Tropical Animal Health and Production - The aim of this study was to determine the prevalence and antimicrobial resistance of mecA and mecC methicillin-resistant Staphylococcus aureus (MRSA) in...  相似文献   

Citations of plenary-session articles published in the Proceedings of the International Symposium on Veterinary Epidemiology and Economics     

Salman MD  Dewell R  Willeberg P  GrandMaison N  Schoenbaum M  Moothart T 《Preventive veterinary medicine》2007,80(1):82-86

We measured the potential impact of articles representing the International Symposium on Veterinary Epidemiology and Economics (ISVEE) plenary-session presentations in subsequent published literature. Between July 1, 2004 and November 9, 2004, we searched the Web of Science for citations in the scientific literature to all 99 plenary-session articles published in the proceedings of the previous nine ISVEEs (or in journal special issues dedicated to the ISVEE plenary articles). We used a 4-year window around the publication of each of the ISVEE proceedings. We located 187 citations for 37 (of the 99) articles. We infer that the ISVEE proceedings represent an important resource for veterinary epidemiology.  相似文献   

Integrated Management of Organic Manures and Chemical Fertilizers for Enhancing Paddy Yield and the Nutrient Content of Rice Cultivars     

Ataollah Ashrafi Esfahani  Hormoz Fallah  Salman Dastan 《Communications in Soil Science and Plant Analysis》2019,50(5):570-585

The integrated nutrient management with organic manure and chemical fertilizers can improve rice (Oryza sativa L.) production, soil health, and fertility. Hence, this study aimed to evaluate the combined effects of organic manures and chemical fertilizers on the yield and nutrient content of wetland rice under field conditions. It was conducted in northern Iran in two consecutive years, 2015 and 2016 as a factorial experiment based on a randomized complete blocks design (RCBD) with three replications. Two local rice cultivars, Tarom Hashemi and Tarom Mahalli, were chosen as the first factor; mineral fertilizers in four levels: 100% recommended nitrogen-phosphorus-potassium (NPK), 75% recommended nitrogen with 100% phosphorus-potassium (PK), 75% recommended phosphorus with 100% nitrogen-potassium (NK), and 75% recommended potassium with 100% nitrogen-phosphorus (NP), were selected based on soil analysis and were arranged as the second factor; and three levels of organic manures, namely zero and eight tons of vermicompost per hectare, and 10 tons of rotted manure per hectare was the third factor. Results revealed that the highest paddy yield occurs with vermicompost and manure consumption for both cultivars. Moreover, the highest paddy yield (3962 kg ha^?1) occurs with 100% recommended NPK and vermicompost consumption. For both cultivars, the most chlorophyll a and chlorophyll ab content was produced with application of 100% recommended NPK. The most grain nitrogen content, grain nitrogen uptake, protein content, and protein yield were observed with 100% recommended NPK applied with 10 tons of rotted manure and eight tons of vermicompost usage per hectare. Therefore, in view of the ever-increasing use of chemical fertilizers and irreversible damage thereby, the additional use of these compounds can benefit the environment and human health. The global attention to sustainable agricultural concepts and organic manure can, in addition to producing reasonable yields, be considered as an appropriate alternative to chemical fertilizers.  相似文献   

Comparative effects of applying leguminous and non-leguminous green manures and inorganic N on biomass yield and nitrogen uptake in flooded rice (<Emphasis Type="Italic">Oryza sativa</Emphasis> L.)     

M.?AshrafEmail author  T.?Mahmood  F.?Azam  R.?M.?Qureshi 《Biology and Fertility of Soils》2004,40(2):147-152

The beneficial role of green manures in rice production is generally ascribed to their potential of supplying plant nutrients, particularly nitrogen (N). However, the mechanisms through which green manures enhance the crop productivity are poorly understood. Pot experiments were conducted using a ¹⁵N-tracer technique: (1) to compare the biomass production potential of sesbania (Sesbania aculeata Pers.) and maize (Zea mays L.) as green manuring crops for lowland rice and (2) to compare the effect of the two types of green manure and inorganic N on the dry matter accumulation and N uptake by two rice (Oryza sativa L.) cultivars, viz. IR-6 and Bas-370. Although maize produced three times higher shoot biomass compared with sesbania, the latter showed higher N concentration; and thus the total N yield was similar in the two types of plants. Applying the shoot material of the two plants to flooded rice significantly enhanced the dry matter yield and N uptake by the two rice cultivars, the positive effects generally being more pronounced with sesbania than with maize amendment. The difference in the growth-promoting potential of the two plant residues was related more to an increased uptake of the native soil N rather than to their direct role as a source of plant-available N. A positive added nitrogen interaction (ANI) was observed due to both plant residues, the effect was much more pronounced with the application of sesbania than with maize residues. In both rice cultivars, inorganic N also caused a substantial ANI, particularly at higher application rate. Losses from the applied N were 2–3 times lower from sesbania, compared with maize treatment. Green manuring with sesbania also caused much lower N losses than the inorganic N applied at equivalent or higher rates. The overall benefit of green manuring to rice plants was higher than inorganic N applied at comparable rates. The two rice cultivars differed in their response to green manuring, IR-6 generally being more responsive than Bas-370.  相似文献   

Genome-wide association analysis for stripe rust resistance in spring wheat(Triticum aestivum L.) germplasm          下载免费PDF全文

Sher MUHAMMAD  Muhammad SAJJAD  Sultan Habibullah KHAN  Muhammad SHAHID  Muhammad ZUBAIR  Faisal Saeed AWAN  Azeem Iqbal KHAN  Muhammad Salman MUBARAK  Ayesha TAHIR  Muhammad Umer  Rumana KEYANI  Muhammad Inam AFZAL  Irfan MANZOOR  Javed Iqbal WATTOO  Aziz-ur REHMAN 《农业科学学报》2020,19(8):2035-2043

Stripe rust is a continuous threat to wheat crop all over the world. It causes considerable yield losses in wheat crop every year. Continuous deployment of adult plant resistance(APR) genes in newly developing wheat cultivars is the most judicious strategy to combat this disease. Herein, we dissected the genetics underpinning stripe rust resistance in Pakistani wheat germplasm. An association panel of 94 spring wheat genotypes was phenotyped for two years to score the infestation of stripe rust on each accession and was scanned with 203 polymorphic SSRs. Based on D' measure, linkage disequilibrium(LD) exhibited between loci distant up to 45 c M. Marker-trait associations(MTAs) were determined using mixed linear model(MLM). Total 31 quantitative trait loci(QTLs) were observed on all 21 wheat chromosomes. Twelve QTLs were newly discovered as well as 19 QTLs and 35 previously reported Yr genes were validated in Pakistani wheat germplasm. The major QTLs were QYr.uaf.2 AL and QYr.uaf.3 BS(PVE, 11.9%). Dissection of genes from the newly observed QTLs can provide new APR genes to improve genetic resources for APR resistance in wheat crop.  相似文献   

Oxidative responses in ferret macrophages     

Cross ML  Qureshi T  Mackintosh CG 《Veterinary immunology and immunopathology》1999,67(2):171-184

Although the basic function of T and B lymphocytes in ferrets has been known for some time, the function of mononuclear phagocytes has not been described in this species. The present study has characterised basic oxidative responses in ferret macrophages, and has investigated the effects of endogenous and exogenous modulators of macrophage function on oxidative capacity in vitro. Macrophages derived from the blood or lungs of ferrets were shown capable of generating the reactive oxygen intermediate (ROI) molecules superoxide and hydrogen peroxide, and secreting a lysosomal enzyme (acid phosphatase), in response to appropriate stimuli. A T cell supernatant (derived from mitogen-stimulated peripheral blood lymphocytes) was able to activate both blood- and lung-derived macrophages for enhanced ROI production, while specific ROI inhibitors (superoxide dismutase and catalase) were able to partially ablate ROI activity. The accumulation of nitrite in culture supernatants, as an indicator for the production of reactive nitrogen intermediates, could not be demonstrated by ferret macrophages derived from either tissue source. In contrast to the enhancing effects of TCS on the oxidative function of blood-derived macrophages, exposure to bacterial LPS caused marked suppression of ROI and lysosomal enzyme production by these cells. Finally, the generation of superoxide anion, following phagocytosis of live or heat-killed Mycobacterium bovis or zymosan, indicated that ROI production in response to phagocytic stimulation was relatively weak in ferret blood-derived macrophages. These results are discussed in relation to the study of immune function in a novel species, and with particular reference to research into tuberculosis (Tb), since ferrets are important wildlife vectors of bovine Tb in New Zealand.  相似文献   

Effects of organic forms of zinc on growth performance,tissue zinc distribution,and immune response of weanling pigs   总被引：9，自引：0，他引：9  

van Heugten E  Spears JW  Kegley EB  Ward JD  Qureshi MA 《Journal of animal science》2003,81(8):2063-2071

This study was conducted to determine the effect of zinc level and source on growth performance, tissue Zn concentrations, intracellular distribution of Zn, and immune response in weanling pigs. Ninety-six 3-wk-old crossbred weanling pigs (BW = 6.45 +/- 0.17 kg) were assigned to one of six dietary treatments (four pigs per pen, four replicates per treatment) based on weight and litter origin. Treatments consisted of the following: 1) a corn-soybean meal-whey diet (1.2% lysine) with a basal level of 80 ppm of supplemental Zn from ZnSO4 (control; contained 104 ppm total Zn); 2) control + 80 ppm added Zn from ZnSO4; 3) control + 80 ppm added Zn from Zn methionine (ZnMet); 4) control + 80 ppm added Zn from Zn lysine (ZnLys); 5) control + 40 ppm added Zn from ZnMet and 40 ppm added Zn from ZnLys (ZnML); and 6) control + 160 ppm added Zn from ZnSO4. Zinc supplementation of the control diet had no effect on ADG or ADFI. Gain efficiency was less (P < 0.05) for pigs fed 80 ppm of Zn from ZnSO4 than for control pigs and pigs fed 160 ppm of Zn from ZnSO4. Organ weights, Zn concentration, and intracellular distribution of Zn in the liver, pancreas, and spleen were not affected (P = 0.12) by Zn level or source. Skin thickness response to phytohemagglutinin (PHA) was not affected (P = 0.53) by dietary treatment. Lymphocyte proliferation in response to PHA was greater (P < 0.05) in pigs fed ZnLys than in pigs fed the control diet or the ZnML diet; however, when pokeweed mitogen was used, lymphocyte proliferation was greatest (P < 0.05) in pigs fed the ZnMet diet than pigs fed the control, ZnLys, ZnML, or 160 ppm ZnSO4 diets. Antibody response to sheep red blood cells was not affected by dietary treatments. Supplementation of 80 ppm of Zn from ZnSO4 or ZnMet and 160 ppm of Zn from ZnSO4 decreased (P < 0.05) the antibody response to ovalbumin on d 7 compared with control pigs, but not on d 14. Phagocytic capability of peritoneal exudate cells was increased (P < 0.05) when 160 ppm of Zn from ZnSO4 was supplemented to the diet. The number of red blood cells ingested per phagocytic cell was increased (P < 0.05) in pigs fed the diet supplemented with a combination of ZnMet and ZnLys and the diet with 160 ppm of Zn from ZnSO4. Results suggest that the level of Zn recommended by NRC for weanling pigs was sufficient for optimal growth performance and immune responses, although macrophage function may be enhanced at greater levels of Zn. Source of Zn did not alter these measurements.  相似文献   

Analysis methods for evaluating bacterial antimicrobial resistance outcomes     

Wagner BA  Dargatz DA  Morley PS  Keefe TJ  Salman MD 《American journal of veterinary research》2003,64(12):1570-1579

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Think screening unnecessary,unrealistic for all blood donors     

Whittemore JC  Salman MD 《Journal of the American Veterinary Medical Association》2003,222(11):1502; author reply 1502

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