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1.
Chhaya P. Sonekar Satyajit Kale Smita Bhoyar Neha Paliwal S. V. Shinde S. P. Awandkar Wiqar Khan S. P. Chaudhari N. V. Kurkure 《Tropical animal health and production》2018,50(1):91-96
Brucellosis is a zoonotic disease worldwide distributed and having the economic as well as public health importance. The prevalence of brucellosis among sheep flock having history of abortions was studied. A total of 229 samples comprising of 157 blood and 72 clinical samples (vaginal swabs) were collected from 157 animals. Clinical samples were processed for the isolation of Brucella melitensis. Serum samples (n = 157) were tested by Rose Bengal plate test (RBPT) and i-ELISA. A total of 68 (43.31%) and 104 (66.24%) samples were positive by RBPT and ELISA, respectively. Brucella isolates (n = 2) were recovered from clinical samples. Both isolates demonstrated amplification for bcsp 31 and IS711 genes. On AMOS PCR, both the isolates amplified at 731 bp, i.e., belongs to B. melitensis species. The incidence of B. melitensis in a migratory flock warns the thorough testing and culling of Brucella-infected sheep from the flock on a continuous basis; otherwise, such incidence will be routine and poor farmers will be at a loss. 相似文献
2.
Adam C. Margetts Emma Scurrell Smita Das Kerry M. Smith 《Veterinary ophthalmology》2023,26(3):238-242
A 10-month-old female, neutered French Bulldog presented with a history of unilateral right-sided intermittent conjunctivitis and exophthalmos. The patient suffered blunt force trauma to the right eye after a tennis ball impact approximately five months prior to presentation. Examination identified the patient was visual with exophthalmos, lateral strabismus, conjunctival hyperaemia, episcleral congestion, and papilloedema. Magnetic resonance imaging and ultrasonography identified an approximately two centimeter diameter fluid-filled structure directly posterior to the globe leading to displacement of the optic nerve and distortion of the posterior globe wall. Centesis of the lesion demonstrated neutrophilic and macrophagic inflammation with evidence of prior hemorrhage. Within four weeks, the structure had re-filled to its original size and was subsequently excised in its entirety, via a lateral orbitotomy. Histopathologic findings indicated a non-keratinising orbital cyst, the lining of which was consistent with a respiratory epithelial cyst. Recurrence had not occurred seven months' post-surgery. To the author's knowledge, previous reports of retrobulbar respiratory epithelial cysts have not been documented in animals. 相似文献
3.
Smita JainRafat S. Ahmed Vinod Kumar AroraBasu Dev Banerjee 《Pesticide biochemistry and physiology》2011,100(2):182-186
Triazophos, O,O-diethyl-1-H-1,2,4-triazol-3-yl phosphorothioate, (TZ) is an organophosphorus pesticide which is extensively used in agriculture for controlling insect pests. Except a FAO/WHO report no study has investigated its short-term toxicity with respect to its potential to cause biochemical and histopathological alterations. The present study was designed to identify the effect of TZ at different doses (1.64, 3.2 and 8.2 mg/kg) on the oxidative stress parameters in blood as well as organs involved in xenobiotic metabolism (liver and brain) following chronic exposure for 90 days. Moreover, the study also delineates the effect of TZ on the histo-architecture of these organs. The results indicated a dose dependent induction (p < 0.001) of oxidative stress, as evident by increased malondialdehyde (MDA) level and compromised antioxidant defense including glutathione S transferase (GST) activity, glutathione (GSH) content and ferric reducing ability of plasma (FRAP) in blood, and increased MDA level with concomitantly decreased GSH content in tissues, following chronic exposure to TZ. The ratio of MDA: FRAP in blood was found to be increased following chronic exposure to TZ and may serve as a suitable indicator of severity of oxidative damage. Onset of such biochemical alterations is one of the early adaptive responses to TZ exposure which leads to histopathological alterations in terms of diffuse fatty changes expanding from mid-zonal area to whole lobule in liver. However, increased oxidative stress did not bring any morphological alteration in brain. The present study concludes that induction of oxidative stress, leading to subsequent histopathological alterations in liver, is an important mechanism underlying the TZ induced chronic toxicity. 相似文献
4.
Citrus irrigation scheduling is usually based on evapotranspiration (ET) multiplied by a crop coefficient that varies throughout the year. However, ET at 10°C and less has not been investigated. Citrus acclimate to temperatures below 10°C, which affects ET, and therefore may allow irrigation scheduling to be adjusted accordingly. Three separate growth chamber experiments were conducted in complete block design with two temperature treatments and 8-one tree replications with the objective of determining water use of ‘Hamlin’ orange exposed to cold temperatures. The treatments included: full cold-acclimating temperatures, alternating 10 days cold and 3 days warm temperatures, and alternating 10 days warm and 3 days cold temperatures. Although well-watered, trees exposed to temperatures ≤10°C demonstrated lower water use compared to trees held at temperatures that promoted growth. Reduction of water use of cold-treated plants than the controls was 66, 20–57, and 14–28% during full cold-acclimating temperatures, alternating 10 days cold and 3 days warm, and alternating 10 days warm and 3 days cold temperatures, respectively. Reduced water use of cold-treated plants was due to stomatal closure, increased root resistance, and decreased leaf area. Effective irrigation scheduling based on water requirements as they change during cold-acclimating temperatures should save water while providing adequate water for yield and quality. 相似文献
5.
K G Padmakumaran Nair T Rajamohan P A Kurup 《Plant foods for human nutrition (Dordrecht, Netherlands)》1999,53(2):133-144
Feeding coconut kernel along with coconut oil in human volunteers has been found to reduce serum total and LDL cholesterol when compared to feeding coconut oil alone. This effect of the kernel was also observed in rats. Since many plant proteins have been reported to exert a cholesterol lowering effect, a study was carried out on the effect of isolated kernel protein in rats. Feeding kernel protein resulted in lower levels of cholesterol, phospholipids and triglycerides in the serum and most tissues when compared to casein fed animals. Rats fed kernel protein had (1) increased hepatic degradation of cholesterol to bile acids, (2) increased hepatic cholesterol biosynthesis, and (3) decreased esterification of free cholesterol. In the intestine, however, cholesterogenesis was decreased. The kernel protein also caused decreased lipogenesis in the liver and intestine. This beneficial effect of the kernel protein is attributed to its very low lysine/arginine ratio 2.13% lysine and 24.5% arginine. 相似文献
6.
Oral treatment with 50 mg X kg(-1) day(-1) of crude methanol extract of Centella asiatica for 14 days significantly increased the anti-oxidant enzymes, like superoxide dismutase (SOD), catalase and glutathione peroxidase (GSHPx), and anti-oxidants like glutathione (GSH) and ascorbic acid decreased in lymphoma-bearing mice. 相似文献
7.
Surra, caused by Trypanosoma evansi, is an economically important veterinary disease of the tropics. Lack of effective drugs or vaccines have made surra a severe economic burden particularly in Asia and sub-Saharan Africa. In this study, a naked DNA construct encoding full length T. evansi beta (β) tubulin gene was used to immunize mice, to elicit a T. evansi β tubulin protein specific humoral immune response, delineated by ELISA. The serum cytokine profile post immunization, as determined by flow cytometry bead based assay, showed a predominant T helper cell Type 1 (Th1) response with significant increase in levels of IFNγ and TNFα. Lethal challenge with T. evansi blood-form trypomastigotes post immunization generated a β tubulin specific recall response and a stronger Th1 type serum cytokine profile which correlated with an extended survival and better control of parasitemia in the immunized mice. 相似文献
8.
Stars and gas in galaxies, hot intracluster medium, and intergalactic photo-ionized gas make up at most half of the baryons that are expected to be present in the universe. The majority of baryons are still missing and are expected to be hidden in a web of warm-hot intergalactic medium. This matter was shock-heated during the collapse of density perturbations that led to the formation of the relaxed structures that we see today. Finding the missing baryons and thereby producing a complete inventory of possibly the only detectable component of the energy-mass budget of the universe is crucial to validate or invalidate our standard cosmological model. 相似文献
9.
Shankar G. Aggarwal Chandra K. Chandrawanshi Raj M. Patel Smita Agarwal Anand Kamavisdar Girdhar L. Mundhara 《Water, air, and soil pollution》2001,130(1-4):855-862
The results of the seasonal analysis of the chemistry of rain, fog and surface water of central India for the two years: June 1996 to May 1998 are presented. A total of 636 rain water samples, 146 fog water samples and 226 surface water samples of 8 locations: namely, Ambikapur, Baikunthpur, Korba, Bilaspur, Raipur, Bhilai, Dallirajhara and Kanker, from different sites were collected for the present study. This paper documents the occurrence of acidic rain and fog events and their effect on surface water pH and aquatic life in central India. Most of the rain and fog water samples collected from Baikunthpur and Korba sites were found to be acidic in nature (i.e. pH < 5). The volume weighted mean pH of rain and fog water samples of these sites was 4.8 and 4.4 with lowest pH 4.4 and 4.0, respectively. However, samples from Ambikapur, Bilaspur, Raipur and Bhilai were slightly acidic and their pH values were always around 5.8. One reservoir (mean pH 5.8) and two stop dams (mean pH 4.4 and 4.2) in Baikunthpur area were found to be acidic. The effect of acidification of water on metal-accumulation in muscles of fishes was studied. Concentrations of Al, Hg, Mn, Cd and Pb were higher in fish from acid, than from less acid waters. Causes of acidification of the atmospheric water and surface water are critically discussed. 相似文献
10.
N. S. Shekhawat Sharley Mohnot Mahendra Phulwaria Harish Smita Shekhawat 《Journal of Sustainable Forestry》2013,32(7):620-632
Salvadora oleoides is an ecologically important multipurpose tree of the arid forest that occurs in saline areas of northwest India. The seed of this plant yields non-edible commercially usable oil. Poor seed germination, low seed viability, and increasing industrialization are some of the constant factors which significantly affect the status of the natural population of this plant. Therefore, there is a great need to develop an efficient propagation system using the tissue culture technique. In the present communication, we demonstrate the development of an in vitro propagation system for S. oleoides. Multiple shoots were induced from nodal segments harvested from about 25- to 30- yr-old lopped trees of S. oleoides on MS medium + 0.1 mg L?1 NAA (Naphthalene acetic acid) + 2.5 mg L?1 BA (6-Benzylaminopurine) + additives. The shoots were multiplied by (a) repeated transfer of the mother explants on MS medium + 1.0 mg L?1 BA + 0.1 mg L?1 NAA + additives and (b) subculturing of shoot on MS + 1.0 mg L?1 BA + additives. About 84% shoots rooted ex vitro on soilrite within 3–4 weeks when base (4–5 mm) of shoots was treated with 100 mg L?1 of IBA (Indole-3-butyric acid) for 5 min. The plantlets were hardened successfully in the greenhouse and transferred to the pots and field. To the best of our knowledge, this is the first report of a regeneration protocol for S. oleoides from explants obtained from mature trees. Use of the ex vitro rooting technique for plant production serves as a more economical option as it reduces labor, cost, and time. We suggest that the methods developed and described in this article can be used for large-scale plant production and conservation of germplasm of this tree species. 相似文献