Bumblebee ocelli and navigation at dusk     

Wellington WG 《Science (New York, N.Y.)》1974,183(4124):550-551

Western bumblebees fly straight while homing by polarized light, but zigzag when they use landmarks. That flight difference was used to determine the roles of the dorsal ocelli and parts of the compound eyes in homing. Polarized light and ocelli can prolong foraging at twilight, when landmarks are no longer visible.  相似文献   

A practical and reliable procedure for in vitro induction of tetraploid tomato     

Milene Miranda Praa  Carlos Roberto Carvalho  Wellington Ronildo Clarindo 《Scientia Horticulturae》2009,122(3):501-505

A relatively rapid, practical and reliable procedure for in vitro production of tetraploid tomato using colchicine is described. Using this procedure 11.11% generation of tetraploids was obtained after exposure of seedling shoot meristem explants with 8 mM colchicine for 96 h. Confirmation of tetraploid production (2n = 48), was determined by flow cytometry and verified by cytogenetic analysis of root tip preparations. The results indicate that the procedure is adequate for induction and screening of tetraploid tomato plantlets.  相似文献   

一种确定林业发展项目的有效工具(英文)     

马焕成 《林业研究》2003,(1)

在云南省绿春县的有些村庄,由砍柴造成的森林破坏是造成环境退化的主要原因。在黑河流域的上游,不断加剧的水土流失和河床抬升对下游上百万的人们构成威胁。尽管人们对森林破坏和土地的可持续利用、社区可持续发展之间的关系不是不了解,但当生存压力存在的时候就会忽略由此造成的对未来的影响。对于村民来说,他们已经意识到了他们的生存状况,并讨论解决的有效途径,而不是单纯等待外界的援助和干预。问题的关键是如何将这些变化和影响以一种直观和形象的方式向村民解释,并且让包括文盲在内的所有人都能理解。从这方面考虑,图形的东西比文字和数字分析更有优势。本文采用MIGIS(移动交互式地理信息系统的英文缩写,它包括两部分的内容,即PLA-参与式学习和行动和GIS-地理信息系统)在云南省绿春县哈尼族村寨来协助进行村民自发的造林规划。图6表1参16。  相似文献   

Determination of skin threshold concentration of an aqueous house dust mite allergen in normal dogs     

J Wellington  W H Miller  J M Scarlett 《The Cornell veterinarian》1991,81(1):37-42

Fifty-six healthy dogs with no known history of atopic disease to indoor allergens were skin tested with 6 different dilutions of an aqueous house dust mite extract. A concentration of 31.25 PNU/ml was found to be the maximum, nonirritating concentration. Thirty-two percent of the research dogs used had to be excluded because they failed to show reactions to any test dilution.  相似文献   

BOVINE BACILLARY HAEMOGLOBINURIA IN VICTORIA     

N. A. M. Wellington  B.V.Sc. A. Perceval  B.Sc.† 《Australian veterinary journal》1966,42(4):128-130

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Effect of varying live weight and preslaughter fast period on the slaughter, storage, cutting and cooking losses of lambs     

R George  D E Hogue  J R Stouffer  G H Wellington 《Journal of animal science》1966,25(1):192-198

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EHV-1 and EHV-4 infection in vaccinated mares and their foals     

Foote CE  Love DN  Gilkerson JR  Wellington JE  Whalley JM 《Veterinary immunology and immunopathology》2006,111(1-2):41-46

A silent cycle of equine herpesvirus 1 infection was described following epidemiological studies of unvaccinated mares and foals on a Hunter Valley stud farm. Following the introduction of routine vaccination with an inactivated whole virus equine herpesvirus 1 (EHV-1) and equine herpesvirus 4 (EHV-4) vaccine in 1997, a subsequent study identified excretion of EHV-1 and EHV-4 in nasal swab samples tested by PCR from vaccinated mares and their unweaned, unvaccinated foals. The current sero-epidemiological investigation of vaccinated mares and their young foals found serological evidence of EHV-1 and EHV-4 infection in mares and foals in the first 5 weeks of life. The results further support that EHV-1 and EHV-4 circulate in vaccinated populations of mares and their unweaned foals and confirms the continuation of the cycle of EHV-1 and EHV-4 infection.  相似文献   

GBP5 promotes NLRP3 inflammasome assembly and immunity in mammals     

Shenoy AR  Wellington DA  Kumar P  Kassa H  Booth CJ  Cresswell P  MacMicking JD 《Science (New York, N.Y.)》2012,336(6080):481-485

Inflammasomes are sensory complexes that alert the immune system to the presence of infection or tissue damage. These complexes assemble NLR (nucleotide binding and oligomerization, leucine-rich repeat) or ALR (absent in melanoma 2-like receptor) proteins to activate caspase-1 cleavage and interleukin (IL)-1β/IL-18 secretion. Here, we identified a non-NLR/ALR human protein that stimulates inflammasome assembly: guanylate binding protein 5 (GBP5). GBP5 promoted selective NLRP3 inflammasome responses to pathogenic bacteria and soluble but not crystalline inflammasome priming agents. Generation of Gbp5(-/-) mice revealed pronounced caspase-1 and IL-1β/IL-18 cleavage defects in vitro and impaired host defense and Nlrp3-dependent inflammatory responses in vivo. Thus, GBP5 serves as a unique rheostat for NLRP3 inflammasome activation and extends our understanding of the inflammasome complex beyond its core machinery.  相似文献   

First feeding of diploid and triploid yellowtail tetra Astyanax altiparanae: An initial stage for application in laboratory studies          下载免费PDF全文

Rafaela Manchin Bertolini  José Augusto Senhorini  Nivaldo Ferreira do Nascimento  Matheus Pereira‐Santos  Laura Satiko Okada Nakaghi  Wellington Adriano Moreira Peres  Regiane Cristina da Silva  George Shigueki Yasui 《Aquaculture Research》2018,49(1):68-74

In this study, the aim was to establish a protocol for first feeding of diploid and triploid yellowtail tetra Astyanax altiparanae in laboratory conditions. The fry were fed with five different diets: (i) Artemia franciscana nauplii, (ii) plankton, (iii) dry food, (iv) Artemia franciscana nauplii + plankton, and (v) Artemia nauplii + plankton + dry food. Additionally, the growth and survival rates of diploid and triploid individuals were also evaluated. On day 10, the length of the fish between the treatments differed significantly (p = .0001) and ranged from 4.07 ± 0.06 mm (dry food) to 8.50 ± 0.64 mm (plankton + Artemia). The sizes of the fish increased with time, except for the fish fed with dry food. The survival rates were similar for the fish fed with the four diets and ranged from 80.7 ± 5.4% (dry food + plankton + Artemia to 92.0 ± 1.6% (plankton + Artemia), but differed from the fish fed with dry food (17.7 ± 5.8%, p = .0017). Diploids and triploids did not present differences on day 0 (p = .2252) and on day 10 (p = .4844) when the fish presented 6.77 ± 0.25 mm and 6.54 ± 0.15 mm respectively. Survival of diploids (87.3 ± 5.13%) and triploids (74.67 ± 2.30%) were also similar (p = .0285). These data are innovative and useful for establishing protocols for this species in both academic and applied sciences.  相似文献   

Equine herpesvirus 1 glycoprotein D expressed in E. coli provides partial protection against equine herpesvirus infection in mice and elicits virus-neutralizing antibodies in the horse     

Weerasinghe CU  Learmonth GS  Gilkerson JR  Foote CE  Wellington JE  Whalley JM 《Veterinary immunology and immunopathology》2006,111(1-2):59-66

The envelope glycoprotein D of EHV-1 (EHV-1 gD) is essential for virus infectivity and entry of virus into cells and is a potent inducer of virus-neutralizing antibody. In this study, truncated EHV-1 gD (gDt) was expressed with a C-terminal hexahistidine tag in E. coli using a pET vector. Western blot analysis using an anti-gD monoclonal antibody demonstrated the presence of gDt bands at 37.5, 36, 29.5 and 28 kDa. The immunogenicity and protective efficacy of partially purified gDt was compared with gD expressed in insect cells by a recombinant baculovirus (Bac gD) using a BALB/c mouse model of EHV-1 respiratory infection. The proteins were also compared in a prime-boost protocol following an initial inoculation with gD DNA. gDt elicited similar levels of gD-specific antibody and neutralizing antibody compared with Bac gD and also provided a similar level of protection against EHV-1 challenge in mice. Inoculation of horses with gDt elicited EHV-1 gD-specific antibodies including virus-neutralizing antibody, suggesting that despite the lack of glycosylation, E. coli may be a useful vehicle for large scale production of EHV-1 gD for vaccine studies.  相似文献