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广西玉林市番石榴焦腐病的病原菌鉴定 总被引：1，自引：0，他引：1
In October 2019, guava (Psidium guajava) fruits were collected from Yulin, Guangxi, after kept indoors for 2-3 days, brown lesions were found on the blossom-end of the fruits with water-soaked haloes around the lesions. A lot of needle tip-sized dots produced at the centre of the lesions, which were subglobose pycnidia, and the conidia were colorless and nearly fusiform. Single spore strains were obtained by isolation and purification, and YLWB-BD01 was selected as representative strain for pathogenicity assay. After wound inoculation, similar symptoms with natural occurrence appeared. The fungus had the same morphological characteristics with YLWB-BD01 was re-isolated from the lesions. BLAST analysis of ITS, EF-1α and β-Tub sequences of YLWB-BD01 shown 100% homology with Botryosphaeria dothidea (Moug.) Ces. & De Not. Phylogenetic tree constructed by the concatenated sequences of ITS～EF-1α～β-Tub sequences shown that the strain YLWB-BD01 was clustered together with B. dothidea (CMW8000). Based on the morphological characteristics and phylogenetic analysis results, the pathogenic fungus causing guava black-rot was identified as B. dothidea. To our knowledge, this is the first report of guava black-rot caused by B. dothidea in China. 相似文献
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自田间采集沃柑黄化植株,盆栽在防虫温室中,二个多月后长出的第一批新梢即恢复绿色,但盆栽两年后再次出现黄化植株。选择盆栽的黄化植株和已恢复绿色的健康植株为材料,通过检测柑桔黄龙病菌、盆栽土壤的pH值和营养物质含量及根系的真菌分子种群,分析沃柑叶片黄化的相关因子,为沃柑黄化现象的综合治理提供依据。经PCR检测,盆栽沃柑健康植株和黄化植株均未感染黄龙病菌;健康植株根系发达,黄化植株根系相对较少。取除根尖外的褐色根段,针对真菌的ITS区域进行高通量测序,发现褐色根段中重要真菌种类是腐皮镰孢菌(Fusarium solani),其相对丰度在健康植株(60.347%)和黄化植株(47.069%)的根段间无显著性差异。盆栽土壤中的pH值及有机质、全氮、碱解氮、速效磷和速效钾的含量也无显著性差异。盆栽的黄化植株经重修剪枝条及断根处理后,长出的新梢叶片恢复健康绿色。可见,在没有感染黄龙病和土壤条件基本一致的条件下,沃柑植株的黄化现象主要是根系稀少及老化,影响了根系的吸收功能而引起的,断根处理可促进新根生长,提高吸收功能,进而使黄化植株新梢恢复健康绿色。 相似文献
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为了解桑里白粉病菌的重寄生真菌种类,通过分离和寄生性验证,获得2个桑里白粉病菌重寄生真菌菌株HP8和HP9,这两个菌株寄生闭囊壳后,子囊及子囊孢子溃解。菌株HP8菌落绿色,菌丝体无色至褐色,菌丝有隔膜,分生孢子梗结节状膨大,分生孢子椭圆形、单胞、褐色或无色,ITS序列（MT463536）与尖孢枝孢Cladosporium oxysporum（MF135506）同源性为100%,系统进化树分析发现菌株HP8与尖孢枝孢（MF135506）聚在一起;菌株HP9菌落白色,菌丝无色,分生孢子月牙形、单胞、无色,ITS序列（MT463537）与刀孢蜡蚧菌Lecanicillium psalliotae（KC881072）同源性为99%,在系统进化树中,菌株HP9与刀孢蜡蚧菌（KC881072）聚在一起。结合形态特征和ITS序列分析,鉴定出菌株HP8和HP9分别为尖孢枝孢和刀孢蜡蚧菌。高通量测序结果表明,病桑叶在室内保存4个月后,桑里白粉病菌的重寄生真菌为刀孢蜡蚧菌。 相似文献
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为探索黄化症状与真菌种群的相关性,采集茂谷柑的健康和黄化植株的根系及根区土壤样品,用高通量测序技术,分析其真菌种群。结果发现,所有样品中均以子囊菌门粪壳菌纲为主。在根系样品中,优势科为丛赤壳科,相对丰度均大于50%,感染黄龙病植株(HM1-R)中,地星科的相对丰度位居第二,为22.58%。在根区土壤样品中,除黄化植株2(HM2-S)的优势科为肉座菌科(相对丰度为30.27%)外,其他样品的优势科均为丛赤壳科。球壳菌科在感染黄龙病植株(HM1-S)、接合菌门未明确科在黄化植株3(HM3-S)均为第二优势科,相对丰度分别为12.93%和16.83%。囊球菌门在所有样品中相对丰度均很低,在0-0.28%之间。可见,不同黄化植株根区土壤样品中,优势科有差异。由此推测茂谷柑黄化症状与根区土壤真菌种群有一定的相关性。 相似文献
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