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Kadsura coccinea (Lem.) A. C. Smith has been wildly cultivated in Southeast of Guizhou province, bearing fruit as an excellent nutrition source being rich in vitamin C, vitamin E, and a variety of trace elements. In 2017-2018, we found twig blight on Kadsura coccinea with small black dot-like structure in the late stage on lesion, a very common disease causing significant economic losses. The fungal isolates were recovered from the symptomatic stem tissues and small black dot (pycnidium) which contained transparent, unicellular and spindle-shaped conidia of (17.4±1.2) μm×(6.5±0.7) μm. The purified culture with the grayish white to dark grayish-green as well as partial straight hyphae on PDA plate was consistent with Neofusicoccum parvum, supported by the aliment and phylogenetic analyses of ITS, Ef1 α and Tub2 gene sequences (GenBank no: MK563984、MK563986 and MK563987).The pathogenicity test on leaves and stems with needle-punching method matched the symptoms described above and re-isolated the fungus confirming the Koch’s postulate. The lethal temperature for mycelial growth was 54℃ for 10 min; The strain could grow normally in most carbon and nitrogen sources except ammonium carbonate. This is the first report of Neofusicoccum parvum causing twig blight on Kadsura coccinea in China. 相似文献
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黑老虎枝枯病病原鉴定及其生物学特性 总被引：1，自引：0，他引：1
<正>黑老虎[Kadsura coccinea (Lem.) A. C. Smith]又名冷饭团、布福娜,果实含丰富的Vc、Ve及多种微量元素,是山区野果珍品[1]。上世纪末,人们从野外引种试栽,目前在黔东南地区实现了规模化栽培。笔者于2017和2018年调查发现,黑老虎枝枯病在黔东南州各种植基地普遍发生,造成严重损失,但关于黑老虎病害的报道甚少[2,3]。本研究对黑老虎枝枯病的病原菌进行了分离培养、柯赫氏法则验证、形态学特征观察及多基因序列 相似文献
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为探究狭叶方竹伴生群落结构特征及优势种空间分布格局,以3个狭叶方竹伴生群落(斗篷山、老山界、雷公山)为研究对象,采用样地法调查分析群落垂直结构、径级结构、高度级结构、物种重要值、优势种空间分布格局及其影响因素。结果表明：3个群落垂直结构明显,从上向下可分为乔木层、灌木层和草本层,层间植物不发达,仅少量藤本和附生草本。3个群落径级结构(DBH≥1 cm)呈倒“J”形,更新良好,群落平均胸径依次为老山界(16.02 cm)>雷公山(11.38 cm)>斗篷山(8.98 cm),群落密度则呈相反趋势。斗篷山、雷公山优势种径级结构呈倒“J”形,种群为增长型。老山界优势种径级结构呈单峰型,光叶水青冈种群为衰退型,多脉青冈和巴东栎种群存在衰退风险。随着群落演替,老山界群落优势种将逐步被替代。3个群落高度级结构总体呈倒“J”形,平均高度大小依次为斗篷山(8.14 m)>雷公山(6.87 m)>老山界(6.69 m)。不同群落优势种高度级结构存在差异,大多呈倒“J”形,单峰型次之。物种重要值在不同群落和群落不同层间存在差异,斗篷山、老山界和雷公山优势种重要值之和分别占乔木层重要... 相似文献
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