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1.
Waner T Nyska A 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1994,23(3):78-80
Serum concentrations of glucose, cholesterol, triglycerides, and serum alkaline phosphatase activity were measured over different periods of time of food deprivation in male rats. Thirty percent of non-fasted rat's sera was found to be lipemic. At 16 hours of fasting, glucose levels dropped by 30% compared to the level of the non-fasting control group, and remained at a relatively constant level for up to 48 hours of fasting. Triglyceride concentrations decreased at 16 hours after fasting. Serum cholesterol levels were not changed at any of the fasting periods compared to the non-fasted control group. Alkaline phosphatase activity was decreased at 8 hours of fasting, with further declines in activity of the serum enzyme seen at 16, 24, and 48 hours of fasting. It was concluded that at 16 to 18 hours fasting, a non-absorptive state had been reached in male rats. 相似文献
2.
Yuval Ramot Michal Steiner Netanel Amouyal Yossi Lavie Guy Klaiman Abraham J. Domb Abraham Nyska Tal Hagigit 《Journal of toxicologic pathology》2021,34(1):11
Fracture-related infections remain a leading cause of morbidity and mortality. We aimed to establish a simple contaminated radial osteotomy model to assess the efficacy of a biodegradable polymer poly(sebacic-co-ricinoleic acid) [p(SA-RA)] containing 20% w/w gentamicin. A unilateral transverse osteotomy was induced in Sprague-Dawley (SD) rats, followed by application of Staphylococcus aureus suspension over the fracture. After successfully establishing the contaminated open fracture model, we treated the rats either systemically (intraperitoneal cefuroxime), locally with p(SA-RA) containing gentamicin, or both. Control groups included non-contaminated group and contaminated groups that were either untreated or treated with the polymer alone. After 4 weeks, the bones were subjected to micro-CT scanning and microbiological and histopathology evaluations. Micro-CT analysis revealed similar changes in the group subjected to both local and systemic treatment as in the non-contaminated control group. Lack of detectable bacterial growth was noted in most animals of the group subjected to both local and systemic treatment, and all samples were negative for S. aureus. Histopathological evaluation revealed that all treatment modalities containing antibiotics were highly effective in reducing infection and promoting callus repair, resulting in early bone healing. While p(SA-RA) containing gentamicin treatment showed better results than cefuroxime, the combination of local and systemic treatment displayed the highest therapeutic potential in this model. 相似文献
3.
Dekker A Dercksen D Snoep J van Wuyjckhuise L 《Tijdschrift voor diergeneeskunde》2007,132(18):695-701
On 26th of july 2007 a new case of bluetongue was notified in the Netherlands and on 2nd of august 2007 foot-and-mouth disease was diagnosed in Surrey, England, which raised the threat of having both infections simultaniously in one area. Bluetongue and foot-and-mouth disease have a different pathogenesis, but symptoms may resemble each other at a later stage of infection. The pathogenesis and possible clinical symptoms of both infections are discussed and illustrated with pictures. 相似文献
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The vertical K-sat of a clay layer, occurring between 30 and 60 cm below the soil surface, was measured in situ in early spring at thirteen sites, using large soil columns. Gypsum was used to form a barrier around the column and K-sat values were measured with an infiltrometer in columns that were first attached and then detached from the subsoil. This procedure allows an estimate of the occurrence of large continuous pores, such as vertical worm channels. Highest values were found in tile-drained grassland, followed by grassland with surface drainage only, and by tile-drained arable land. Relatively low K-sat for the silty subsoil, rather than the (high) vertical K-sat for the clay layer, is considered to be responsible for high groundwater tables in the wet season.Undisturbed, large columns were taken to the laboratory and saturated for a period of three months to simulate prolonged swelling after a very wet season, and to measure chloride-breakthrough curves, for characterizing soil-pore continuity. The clay layer, sampled in the surface-drained grassland, showed no significant reduction of K-sat after prolonged swelling, but the one for arable land was reduced. Moreover, flow in the latter occurred through only a few relatively large, continuous pores, whereas a more heterogeneous pore system was found for the column from grassland. The already high K-sat of the clay layer in surface-drained grassland increased as a result of tile drainage. Compaction of the clay layer in tile-drained arable land reduced K-sat well below the level found in surface-drained grassland. 相似文献
6.
J. G. Wesseling C. R. Stoof C. J. Ritsema K. Oostindie & L. W. Dekker 《Soil Use and Management》2009,25(3):274-283
To gain more insight into the hydrological behaviour of coarse-textured soils, the physical properties of artificially created soil mixtures with different texture were determined. The mixtures were prepared according to the specifications of the United States Golf Association (USGA) for constructing putting greens. In addition, the effect of 10 vol.% organic matter addition was studied. The soil moisture retention and hydraulic conductivity relationships of the different mixtures were determined and their hydrological behaviour was studied using the numerical model SoWaM. Both texture and organic matter addition substantially affected the hydraulic properties. Hydraulic conductivity significantly increased with increasing coarseness while moisture retention decreased. On the other hand, organic matter addition reduced saturated hydraulic conductivity by a factor of 10 to 100 and distinctly increased moisture retention capacity. The amounts of total available water were increased by the addition of organic matter between 144% (slightly coarse texture) and 434% (very coarse texture). Results indicate that the mixtures can contain only 2–16% plant available water and therefore need frequent irrigation to maintain plant growth. Addition of organic matter seems a good solution to reduce the irrigation water requirements but it increases the risk of ponding or runoff because of large reductions in the saturated hydraulic conductivity sometimes to below the rate of 3.6 m/day recommended by the USGA. 相似文献
7.
The Use of Antigen-capture Enzyme-linked Immunosorbent Assay (ELISA) for the Diagnosis of Rinderpest and Peste des Petits Ruminants in Ethiopia 总被引:1,自引:1,他引:0
Rinderpest had been reported in most parts of Ethiopia when the Pan African Rinderpest Campaign (PARC) was launched. As a result of intensive disease investigation and strategic vaccination, most parts of the country are now considered provisionally free, and widespread vaccination has been replaced by clinical and serological surveillance. Details of any episodes of disease are recorded and followed up after laboratory confirmation of suspected cass using antigen-capture ELISA. This paper is based on observations on the performance of the antigen detection ELISA compared to the agar gel immunodiffusion (AGID) test, which also differentiates rinderpest from peste des petits ruminants (PPR). The stability of the specific viral antigen was monitored for 4 days, and rinderpest and PPR antigens were still detected, depending on the type of specimen. Antigen capture ELISA is more rapid, sensitive and virus specific than the AGID. Even if the cold chain of the specimen is compromised for a day or two during sample collection and submission, the specimen may still be suitable for testing by ELISA. 相似文献
8.
An investigation was carried out into an outbreak of respiratory disease complex (RDC) in 3641 Menz and Awassi×Menz cross sheep in Central Ethiopia between 1998 and 1999 by clinical, serological, microbiological, post-mortem and histopathological examinations. The monthly incidence of RDC varied from 2.8% to 4.0% and the prevalence was as high as 17%. The case fatality rate was 18%, despite culling of sick sheep. Over 76% of the morbidity occurred in adults, followed by 19% among weaners. Similarly, 62% of the mortality was in adults. However, 27% of the mortality occurred in lambs despite the low morbidity in the group. Significant breed and age differences were seen in the morbidity and mortality rates (p<0.05). Clinical signs, gross and microscopic lesions and serological and bacteriological examinations showed an interplay of several causes of the RDC, including pestes des petits ruminants (PPR) (72.3%, serologically confirmed), lung worms, maedi-visna, bacterial bronchopneumonia (staphylococcal and streptococcal), enzootic pneumonia and some fungal infections. Cold temperatures, which may be as low as –8.5°C at night, are major predisposing factors along with managemental stresses. Vaccination of animals with a homologous PPR vaccine appeared to decrease dramatically the occurrence of the disease, showing that PPR played an important role in the outbreak. Several of the pathogens do not appear to be individually capable of causing the respiratory disease. Appropriate strategies for the prevention of RDC are suggested. 相似文献
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