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1.
An experiment was carried out to evaluate the response obtained in a three‐way cross of rabbits when the lines involved had undergone within‐line selection for litter size or postweaning daily gain. The interaction between the level of nutrition and genetic level of the crossbreds was also studied. Using frozen embryos, old (H1) and current (H2) crossbred does and young were compared contemporarily. Does were fed a standard diet or a diet having high digestible energy and protein. The difference in the number of generations between lines used to produce both crosses were: 13 or 0 for the maternal lines, and 12 for the paternal line. The traits studied in the crossbred does were: daily feed intake during gestation, during the first 3 weeks of lactation and during the last week before weaning; doe weight at mating and at kindling; litter weight at birth and at weaning; total litter size, number born alive and litter size at weaning. The recorded traits for the young were: individual weight at weaning and at 63 days; individual daily gain after weaning; daily feed intake and feed conversion index. The last two traits were recorded as averages per cage. A total of 1161 litters from 190 does were involved. Significant differences in litter‐size traits, between H1 and H2 does, were observed and were higher than the expected from the within‐line response. The doe effect was not significant for other doe traits, adjusted to a constant litter size. The estimated difference in postweaning daily gain between H1 and H2 young was significant and in favour of H2, but the value was considerably lower than expected. The effect of type of feed and the interaction with the type of doe was not significant for any doe trait. The type of feed consumed by the doe was significant for postweaning daily gain, and the interaction with the type of crossbreds was significant for this trait and for individual weight at 63 days.  相似文献   
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3.
In the Netherlands the greenhouse sector is a major user of energy. It accounts for 7% of the total national energy use and for 79% of the total energy use in agriculture. In order to sustain this sector on the long term, it is important that its use of energy is lowered. One way of reducing energy use by horticultural producers is investing in energy-saving systems. The purpose of this paper is to provide a better understanding of the investment behavior of firm operators in the adoption of energy-saving systems. Research objectives of the paper are (1) to analyze factors underlying the decision to invest, (2) to explore factors underlying the optimal size of investments. Three investment theories were used to construct an empirical model of investment. Consequently, this model was estimated in a two-stage procedure to analyze the factors influencing the decision of farmers to invest and the level of investments. The paper ends with policy implications.  相似文献   
4.
‘Blanquilla’ pears processed as fresh-cut products are highly sensitive to browning and softening. Common postharvest methods, such as the use of antibrowning compounds and/or modified atmosphere packaging, fail to preserve ‘Blanquilla’ pear slices long enough to be marketable. However, treatment with 1-MCP before cutting and peeling considerably improved their textural properties (9.2 N vs. 1.1 N with and without 1-MCP treatment, respectively) and color (a* values of 1 vs. 5 after 15 d at 4 °C, for slices pear treated with 1-MCP and without treatment, respectively). These positive changes were closely related to a decrease in respiratory activity determined on whole pears after 3 months of storage in air at 0 ± 1 °C and 95% R.H. (0.40 ± 0.05 mmol CO2 kg−1 h−1 vs. 0.77 ± 0.04 mmol CO2 kg−1 h−1 with and without 1-MCP treatment, respectively) and ethylene production (1.18 ± 0.36 nmol C2H4 kg−1 h−1 vs. 5.751 ± 1.12 nmol C2H4 kg−1 h−1 for samples treated with and without 1-MCP, respectively). The use of 1-MCP allows fresh-cut ‘Blanquilla’ pears to be sold up to about 5 d after processing. Treatment with 1-MCP could be a viable alternative to common technologies for extending the shelf-life of ‘Blanquilla’ pears as a fresh-cut product.  相似文献   
5.

Background

Salinity is one of the most severe and widespread abiotic stresses that affect rice production. The identification of major-effect quantitative trait loci (QTLs) for traits related to salinity tolerance and understanding of QTL × environment interactions (QEIs) can help in more precise and faster development of salinity-tolerant rice varieties through marker-assisted breeding. Recombinant inbred lines (RILs) derived from IR29/Hasawi (a novel source of salinity) were screened for salinity tolerance in the IRRI phytotron in the Philippines (E1) and in two other diverse environments in Senegal (E2) and Tanzania (E3). QTLs were mapped for traits related to salinity tolerance at the seedling stage.

Results

The RILs were genotyped using 194 polymorphic SNPs (single nucleotide polymorphisms). After removing segregation distortion markers (SDM), a total of 145 and 135 SNPs were used to construct a genetic linkage map with a length of 1655 and 1662 cM, with an average marker density of 11.4 cM in E1 and 12.3 cM in E2 and E3, respectively. A total of 34 QTLs were identified on 10 chromosomes for five traits using ICIM-ADD and segregation distortion locus (SDL) mapping (IM-ADD) under salinity stress across environments. Eight major genomic regions on chromosome 1 between 170 and 175 cM (qSES1.3, qSES1.4, qSL1.2, qSL1.3, qRL1.1, qRL1.2, qFWsht1.2, qDWsht1.2), chromosome 4 at 32 cM (qSES4.1, qFWsht4.2, qDWsht4.2), chromosome 6 at 115 cM (qFWsht6.1, qDWsht6.1), chromosome 8 at 105 cM (qFWsht8.1, qDWsht8.1), and chromosome 12 at 78 cM (qFWsht12.1, qDWsht12.1) have co-localized QTLs for the multiple traits that might be governing seedling stage salinity tolerance through multiple traits in different phenotyping environments, thus suggesting these as hot spots for tolerance of salinity. Forty-nine and 30 significant pair-wise epistatic interactions were detected between QTL-linked and QTL-unlinked regions using single-environment and multi-environment analyses.

Conclusions

The identification of genomic regions for salinity tolerance in the RILs showed that Hasawi possesses alleles that are novel for salinity tolerance. The common regions for the multiple QTLs across environments as co-localized regions on chromosomes 1, 4, 6, 8, and 12 could be due to linkage or pleiotropic effect, which might be helpful for multiple QTL introgression for marker-assisted breeding programs to improve the salinity tolerance of adaptive and popular but otherwise salinity-sensitive rice varieties.
  相似文献   
6.
Milkfish (Chanos chanos) fry with an average weight of 40 mg per fish were stocked in 60-l wooden-glass aquaria filled with 30 l of filtered sea water with a salinity of 32–34 p.p.t. and a temperature of 25–28°C. They were fed diets containing 20, 30, 40, 50 and 60% protein and 2740 kcal of digestible energy per kg at a daily rate of 10% of the biomass for a period of 30 days. Fish which were fed the diet containing 40% protein had the highest weight gain which was significantly higher (P < 0.05) than those of fish receiving the lower dietary levels of protein. Slightly lower weight gains were obtained when fish were fed diets containing 50 and 60% protein. Although feed conversion values were not statistically different (P < 0.05) among all treatments, the value for the 40% protein diet (1.96) was the best. The mean survival rates were low for all treatments but was highest for the 40% protein diet. No significant difference (P < 0.05) was found in the survival rates of fish receiving different treatments. Results show that a dietary level of 40% protein was required by milkfish fry for maximum growth, efficient feed conversion and high survival rate.  相似文献   
7.
The biological activity of a series of N-(pyrid-3-yl)thioureas and -carbodiimides, analogues of the insecticide/acaricide diafenthiuron, towards the carmine spider mite (Tetranychus cinnabarinus Boisd.) and the two-spotted spider mite (T. urticae Koch) was analysed using QSAR methodology. A canonical correlation analysis allowed for the establishment of a prediction model and the identification of outliers within that model. The chemodynamic behaviour of certain compounds in the two series, including these outliers, determined by using photochemical experiments in the laboratory as well as in the glasshouse, was shown to be responsible for anomalous results obtained in the biological tests. It was found from the above study that the biological activity towards both spider mite species is extremely sensitive to the kinetics of formation of the carbodiimides from the corresponding thioureas and to the photostability of the former. The results obtained with a thiourea which underwent no appreciable photochemical transformation into the corresponding carbodiimide supported the hypothesis that the thioureas are also in-vivo propesticides of the acaricidal carbodiimides.  相似文献   
8.
Primary porcine endothelial cells have a limited life span in culture. After four to five passages, they tend to de-differentiate and eventually reach senescence. The aim of this work was to establish immortalized porcine aortic endothelial cell lines (AOCs) to facilitate in vitro studies of different pathological process involving the endothelium. Primary porcine aortic endothelial cells (PAECs) were transfected with a plasmid containing the SV40 genome and selected on the basis of morphological and phenotypical features. Flow cytometry analysis demonstrated uptake of acetylated low density lipoproteins (Ac-LDL) and constitutive expression of SLA class I, CD29, CD31, CD41/61, CD80/86, CD46, SWC3, and LAMP-1 antigens by all analyzed lines and showed little differences to primary cells. The functional similarity between primary and immortalized endothelial cells was demonstrated in a cytotoxicity assay using a human natural killer cell line (NKL) as effector. The AOCs cell lines should be valuable tools for in vitro study of the human immune response against pig endothelial cells. In addition, they would be very useful to gain insight in the pathogenesis of some viral haemorrhagic diseases of pig such as African swine fever (ASF) or classical swine fever (CSF).  相似文献   
9.
Zusammenfassung Fassen wir die vorliegenden Ergebnisse kurz zusammen, so lassen sich folgende Schlußfolgerungen ziehen:1. Eine direkte Bekämpfung des Kartoffelnematoden ist zur Zeit noch nicht möglich. Eine weitgestellte Fruchtfolge vermag ebenfalls nicht in allen Fällen das Auftreten von Nematodenschäden zu verhindern.2. Die leichten Sandböden sind am stärksten verseucht. Dies zeigt sich besonders deutlich in dem Betrieb I, wo ein großer Teil des Ackerlandes aus Lehm- und schwerem Boden besteht. Hier wird auf den leichteren Böden besonders intensiver Kartoffelanbau betrieben, was zu einer besonders schnellen Verseuchung dieser Flächen führt.3. Bei zentraler Lage des ursprünglichen Verseuchungsherdes innerhalb eines stark arrondierten Betriebes (Betrieb I) ist die Verschleppung von Nematodenzysten von der verseuchten Fläche auf die übrigen Ländereien bedeutend intensiver als in Betrieben mit getrennt liegenden Einzelparzellen, wo der Verseuchungsherd weit vom Hof und dem übrigen betriebseigenen Ackerland entfernt liegt (Betrieb II). Im Betrieb I konnten auf zwei Flächen, die noch nie oder nach Umbruch der Grasnarbe erstmalig mit Kartoffeln bestellt waren, Nematodenzysten festgestellt werden.4. Die Gefahr der Verschleppung von Nematodenzysten durch die an Ackergeräten anhaftende Erde ist durch den verstärkten Einsatz genossenschaftlicher Maschinen, besonders des Kartoffel-Vorratsroders, erheblich größer geworden.5. Betriebe in stark mit Nematoden verseuchten Gebieten sind besonders der Gefahr ausgesetzt, durch den Einsatz genossenschaftlicher Maschinen im eigenen Betrieb oder durch Benutzung betriebseigener Maschinen zu Bestellungs-und Erntearbeiten in Nachbarbetrieben Nematoden von außerhalb in den Betrieb einzuschleppen.6. Auch in Abwässern, Stallmist und Kompost wurden verschiedentlich Kartoffelnematodenzysten gefunden, so daß auch auf diesem Wege eine Weiterverbreitung der Nematoden erfolgen kann. Während der Inhalt der im Kompost vorhandenen Zysten auch nach längerer Lagerzeit der Komposterde noch voll lebensfähig ist, werden durch längere Lagerung im Innern des Stallmiststapels die Nematodenlarven in den Zystenhüllen abgetötet. Den Darmtraktus der Tiere können dagegen die Larven im Schutze der Zystenhülle ungeschädigt passieren.7. Durch Wirtschafts- und Pflanzkartoffeln sowie durch alle übrigen mit Erde behafteten Pflanzen und Pflanzenteile, die in den Handel gelangen, können Nematodenzysten oft über weite Strecken verschleppt werden.8. Durch Beachtung der erwähnten Vorsichtsmaßnahmen kann eine weitere Ausbreitung des Kartoffelnematoden weitgehend verhindert werden.  相似文献   
10.
The presence of the non-indigenous species, the black-pygmy mussel Xenostrobus securis, is reported here for the first time in an intense shellfish farming area off Galicia (NW Spain). Very high concentrations of this mytilid bivalve have colonized estuarine waters located at the inner part of the Ria de Vigo. The invasive role of X. securis is discussed in the context of the wide ecological tolerance of the species and the recent finding of settlements of this species on numerous colonies of the economically-important blue mussel Mytilus galloprovincialis. The mode of introduction of the black-pygmy mussel is also discussed in relation to human management activities.  相似文献   
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