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Cerebral cysticercosis in pigs studied by computed tomography and necropsy   总被引:1,自引:0,他引:1  
An original technique for computed tomography (CT) of the pig's brain is described. Brains of 11 cysticercotic pigs were studied by CT and by macroscopic and microscopic examination after necropsy, in order to compare the tomographic images with the anatomic findings. By CT, cysticerci could be seen in all the brains except one which had only one parasite. Good correlation was found when the CT sections were compared with the anatomic slices, nevertheless not all cysticerci seen during necropsy examination could be identified in the CT images. Only two parasites were found in the ventricles. There were difficulties in differentiating submeningeal and parenchymal localization of the cysticerci. Most cysticerci had similar morphologic appearance; inflammatory reactions of different degrees and characteristics were observed around some of them.  相似文献   
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With the aim of genotyping Echinococcus granulosus cysts found in Mexican livestock, we collected hydatid cysts from the livers and lungs of pigs in slaughterhouses in the state of Morelos, Central Region of Mexico. DNA was extracted from the parasites and examined by polymerase chain reaction (PCR) of rDNA internal transcribed spacer 1 (ITS1-PCR), Eg9-PCR, Eg16-PCR, and PCR-restriction fragment length polymorphism (PCR-RFLP). In addition, fragments of the genes coding for mitochondrial cytochrome c oxidase subunit 1 (CO1) and NADH dehydrogenase 1 (ND1) were sequenced. Two different genotypes of E. granulosus were unequivocally identified, the common sheep genotype, G1, and the common pig genotype, G7. The G1 genotype of E. granulosus has not been previously demonstrated in Mexico. Because of its recognized infectivity in humans, G1 genotype is a direct threat to human health and its presence in Mexico is consequently of immediate public health importance and epidemiological relevance.  相似文献   
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The aim of this work was to analyse the genetic origin of the Mexican Creole donkey, as well as its genetic diversity, by comparison with Spanish and African donkey populations by means of the D-loop region of mitochondrial DNA. To this end, the genomic DNA of 68 Mexican Creole donkeys from eight geographical regions in six States of the Republic of Mexico and from a Sicilian donkey was obtained. By the polymerase chain-reaction technique (PCR) a fragment of 541 bp was amplified, corresponding to the most informative region of the mitochondrial DNA, the D-loop. The fragments were subsequently sequenced. The analysed sequences revealed 10 new Mexican haplotypes that were different from those of the Spanish and African breeds with which they were compared, showing high levels of genetic diversity. Analysis of the phylogenetic relationships in the different Creole varieties showed a tendency of origin towards Spanish breeds, mainly the Andaluza, Zamorano-Leonesa and Majorera from the Canary Islands; these in turn showed an African origin, seven Mexican haplotypes and three haplotypes similar to those analysed by Aranguren and colleagues (2004) of Spanish and African breeds being obtained. This work allows us to reach the preliminary conclusion that the origin of Mexican Creole donkey populations in the different states of the Republic of Mexico is clearly of Iberian origin, the Spanish donkey breed Andaluza being the main one contributing to the populations of the Mexican Creole donkeys, followed by the Spanish breeds Zamorano-Leonesa and Majorera from the Canary Islands, and that the populations possess high levels of genetic diversity.  相似文献   
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Phytoparasitica - Tephritid fruit flies are susceptible to insecticide treatments when leaving infested fruit to pupate in the soil and when emerging as adults. Laboratory experiments involved...  相似文献   
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This study assessed the effects of five different transport periods on physiometabolic responses and gas exchange in ostriches. It included 138 ostriches that were assigned to five experimental groups. G1 included 78 birds that were set aside as a reference group (RG). Each one of the four remaining experimental groups included 20 ostriches, which were transported in the following manner: G1 on one occasion for a period of 1 h; G2 on one occasion for 2 h; and G3 on one occasion for 3 h; G4a was made up of the ostriches from G1 but they were shipped on a second occasion, for 2 h (making a total of 3 h); finally, G4b was that same group, but after a third transport period, on this occasion during 3 h (for a total of 6 h). Groups G2 and G3 presented the most marked blood alterations (P < 0.05), including an increase in pH, hypocapnia (27.8 ± 0.80 mmHg), hypernatremia (171.75 ± 1.84 mmol/L), hypocalcemia (0.95 ± 0.03 mmol/L), and hyperglycemia (224.05 ± 3.94 mg/dL). Also, group G3 presented the lowest hematocrit values (26.5 ± 0.47 HTC%). Therefore, transporting ostriches with no prior experience for 3 h caused the most pronounced physiometabolic changes.  相似文献   
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Body measurements (length from nape of neck to the withers; height to withers; length from withers to tail root; length from shoulder to tuber ischii; thoracic circumference; umbilical circumference) were taken and correlated with live weight from 160 donkeys (mean ± standard deviation = 6 ± 2.6 years old) in Central México. The age was assessed from dentition. Sex of the donkeys was also recorded. Sex was an important factor of variation (p = 0.011). Live weight was estimated using two allometric models. Model 1: Live weight = β o × (thoracic circumference)β1. Model 2: Live weight = β o × (height to the withers) βl × (thoracic circumference) β2. Separate prediction equations were produced for males and females, plus one for the total sampled. The ‘best fit’ models, were those using thoracic circumference to predict the live weight. Males: live weight = 0.018576 × (thoracic circumference)1.84107 (R 2 = 0.9839). Females: live weight = 0.031255 × (thoracic circumference)1.72888 (R 2 = 0.9839). The equations derived to estimate the live weight of donkeys in Britain, Morocco and Zimbabwe were less satisfactory for use with donkeys from Central México because they overestimated the live weight.  相似文献   
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