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Yoshiaki HAYASHI Bhim B. THAPA Mohan P. SHARMA Maheshwor SAPKOTA Hajime KUMAGAI 《Animal Science Journal》2009,80(4):418-427
To identify the effects of whole crop maize silage (MS) as a substitute for rice straw (RS) on feed intake and milk production of mid-late lactating buffalo and cattle in Tarai, Nepal, eight Murrah and eight Jersey-Hariana were fed the basal diet, RS ( ad libitum ) with concentrate (0.68% of bodyweight [BW] on a dry matter [DM] basis). A 4 × 4 Latin square design experiment was conducted in each animal species with graded levels of MS substitution for RS (0%, T1; 33%, T2; 67%, T3 and 100%, T4). The MS had higher digestibility and total digestible nutrient (TDN) than RS. The DM intake per BW of the both species was highest in T3. The substitution of MS for RS increased the crude protein intake and the TDN intake in the both species. Although the buffalo showed the highest milking performance in T4, the cattle showed no significant differences in their milking performance among the treatments. The substitution of MS for RS improved the feed intake and milk production in the buffalo. On the other hand, the milk yield was not raised in the cattle, though the feed intake was increased by the substitution. 相似文献
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Effect of two-component cultivar mixtures on development of wheat yellow rust disease in the field and greenhouse in the Nepal Himalayas 总被引：1，自引：0，他引：1
Bhim Chaulagain Gopal Bahadur Khatri Chhetri Sundar Man Shrestha Sarala Sharma Dipak Sharma-Poudyal Jay Ram Lamichhane 《Journal of General Plant Pathology》2017,83(3):131-139
Wheat yellow rust (WYR), caused by Puccinia striiformis f. sp. tritici (PST), is a major disease of wheat, and deployment of a single cultivar often leads to disease epidemics. Effect of inoculum level, foliar fungicide spray, and wheat cultivar mixtures were evaluated on disease development in the field and greenhouse in Nepal. Treatments were arranged in a split–split plot design with three replications in both experiments. Two inoculum levels of PST (low and high) were main plot factors; nontreated control and foliar spray of fungicides (Mancozeb and Bayleton) were subplot factors; and two-component cultivar mixtures, composed of different ratios of a susceptible (S) and a resistant (R) cultivars (90:10, 80:20, and 50:50, 100:0, and 0:100) were sub–subplot factors. WYR severity was assessed at different time intervals, and disease development was calculated as area under the disease progress curve (AUDPC). Inoculum level did not cause significant differences in AUDPC in the field but did in the greenhouse. Foliar spray of fungicides reduced the AUDPC in the greenhouse and field. In both experiments, AUDPC values were low in cultivar mixtures compared with a pure stand of a susceptible cultivar. As the proportion of resistant cultivar increased compared with the susceptible cultivar in the S:R mixture component, disease severity decreased with a consequent increase in grain yield. The greater yield obtained with cultivar mixtures compared with only the susceptible cultivar, independent of inoculum level and fungicide spray in the field, revealed a promising strategy to manage WYR in Nepal. 相似文献
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Santosh Sanjel Martha Hincapie Yunwen Wang James Todd Bhim Chaulagain Sushma Sood Jack C. Comstock Richard N. Raid Philippe Rott 《Plant pathology》2021,70(7):1616-1625
Orange rust of sugarcane caused by Puccinia kuehnii was first reported in Florida in 2007. Since then, several sugarcane cultivars that were resistant during the initial epidemics became susceptible within a few years. These shifts in resistance were attributed to the evolution of the pathogen and appearance of new races. To study the variation in virulence of P. kuehnii, healthy leaf pieces of sugarcane cultivars susceptible to orange rust were brush inoculated with isolates of P. kuehnii collected from susceptible cultivars in the field. After inoculation, leaf pieces were placed in an incubator and disease severity based on the number of rust uredinia was determined 2 weeks postinoculation. Isolates of P. kuehnii collected from sugarcane cultivar CP 89-2143, which only showed severe symptoms of orange rust starting in 2011–2012, produced 300%–500% more uredinia on CP 89-2143 than the isolates collected from cultivar CL 85-1040 that has been susceptible since 2007. Sugarcane cultivar CL 85-1040 exhibited high and equivalent numbers of uredinia regardless of the inoculated isolate of the pathogen. These data support the occurrence of pathogenic specialization within P. kuehnii and the existence of at least two races of this pathogen in Florida. Analysis of amplified fragment-length polymorphism among isolates of P. kuehnii from cultivars CP 89-2143 and CL 85-1040 differing in resistance to orange rust revealed genetic variation among rust uredinia. However, this variation was not associated with a specific sugarcane cultivar, suggesting that pathogenic variation was not linked to major, but rather to small genetic changes within the genome of P. kuehnii. 相似文献
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Francesca Bampa Lilian O'Sullivan Kirsten Madena Taru Sandn Heide Spiegel Christian Bugge Henriksen Bhim Bahadur Ghaley Arwyn Jones Jan Staes Sylvain Sturel Aneta Trajanov Rachel E. Creamer Marko Debeljak 《Soil Use and Management》2019,35(1):6-20
Soil and its ecosystem functions play a societal role in securing sustainable food production while safeguarding natural resources. A functional land management framework has been proposed to optimize the agro‐environmental outputs from the land and specifically the supply and demand of soil functions such as (a) primary productivity, (b) carbon sequestration, (c) water purification and regulation, (d) biodiversity and (e) nutrient cycling, for which soil knowledge is essential. From the outset, the LANDMARK multi‐actor research project integrates harvested knowledge from local, national and European stakeholders to develop such guidelines, creating a sense of ownership, trust and reciprocity of the outcomes. About 470 stakeholders from five European countries participated in 32 structured workshops covering multiple land uses in six climatic zones. The harmonized results include stakeholders’ priorities and concerns, perceptions on soil quality and functions, implementation of tools, management techniques, indicators and monitoring, activities and policies, knowledge gaps and ideas. Multi‐criteria decision analysis was used for data analysis. Two qualitative models were developed using Decision EXpert methodology to evaluate “knowledge” and “needs”. Soil quality perceptions differed across workshops, depending on the stakeholder level and regionally established terminologies. Stakeholders had good inherent knowledge about soil functioning, but several gaps were identified. In terms of critical requirements, stakeholders defined high technical, activity and policy needs in (a) financial incentives, (b) credible information on improving more sustainable management practices, (c) locally relevant advice, (d) farmers’ discussion groups, (e) training programmes, (f) funding for applied research and monitoring, and (g) strengthening soil science in education. 相似文献
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Ajit Kumar Passari P. C. Lalsiamthari Zothanpuia Vincent Vineeth Leo Vineet Kumar Mishra Mukesh Kumar Yadav Vijai Kumar Gupta Bhim Pratap Singh 《European journal of plant pathology / European Foundation for Plant Pathology》2018,150(4):831-846
In the present study, 129 rhizospheric bacteria isolated from Curcuma longa were screened for their antagonistic potential against six fungal phytopathogens. Among them, 32 isolates that showed significant antagonistic potential were screened for their in vitro plant growth promoting (PGP) traits. The identification of potential isolates was confirmed by 16S rRNA gene sequencing and results revealed Bacillus as the dominant genus followed by Staphylococcus, Pseudomonas, Sphingomonas and Achromobacter. Based on the antagonistic activity and PGP traits; two strains (BPSRB4 and BPSRB14), identified as Bacillus amyloliquefaciens, were further tested for their in vivo PGP and disease suppression potential on Capsicum annuum seedlings under greenhouse conditions. The results demonstrated that BPSRB4 and BPSR14 strains suppress fungal pathogen infection and promote plant growth. Further, the BPSRB4 strain was positive for the production of the phytohormone indole acetic acid (IAA) detected by thin layer chromatography (TLC). In addition, nitrogen fixation and plant growth promotion activity were also confirmed by amplification and sequencing of nitrogen fixation gene (nifH) and ACC (1-aminocyclopropane-1-carboxylate) deaminase (acdS) gene from strains BPSRB4 and BPSRB14. The present study demonstrated that the B. amyloliquefaciens strains BPSRB4 and BPSR14 possess antagonistic activity and PGP potential which could be explored for the development of biofertilizers and biocontrol agents for the growth of chilli seedlings. 相似文献
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Human-killing is the ultimate expression of human-tiger conflict. It is a complex issue that needs to be addressed to maintain support for tiger (Panthera tigris) conservation in areas where human-killing is prevalent. This research was undertaken to investigate the ecological and sociological aspects of human-killing in the central lowlands of Nepal. We used 28 years of data from human-killing events in Chitwan National Park and the surrounding area to: (1) document the geographic distribution of human-killing incidents, (2) examine ecological variables associated with sites where humans were killed, (3) characterize human-killing tigers, and (4) identify human activities that make people vulnerable to attack. Finally, we use this information to recommend strategies to reduce human-tiger conflicts. Data on human-killing incidents and removal of human-killing tigers were obtained from veterinarian and Kathmandu Zoo records and by visiting the location of each kill with a victim’s family member or friend. Thirty-six tigers killed 88 people from 1979 to 2006. Most (66%) kills were made within 1 km of forest edge but equally in degraded and intact forests. An equal number of male and female tigers killed humans and 56% of tigers that were examined had physical deformities. The trend of human deaths increased significantly from an average of 1.2 (±1.2) persons per year prior to 1998 to 7.2 (±6.9) per year from 1998 to 2006. This difference is due primarily to a ten fold increase in killing in the buffer zone since 1998 because of forest restoration. Nearly half the people killed were grass/fodder collectors. Local participation in tiger management and conservation is essential to mitigate human-tiger conflicts. We recommend that villagers be recruited to help radio collar and monitor potentially dangerous tigers, participate in long term tiger monitoring, and attend a tiger conservation awareness program focused on tiger behavior and avoidance of conflict. 相似文献
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With an objective to evaluate the Bhutan rice (Oryza sativa L.) landraces for genetic diversity in blast resistance, 402 accessions viz. 352 landraces, a differential set of 32 R gene
lines and 18 modern cultivars were field-evaluated in three blast ‘hotspot’ sites followed by genetic analysis of the 352
landraces with 27 microsatellite markers. Across the sites, 19 landraces (5.4%) exhibited complete resistance with zero disease
score and 203 (58%) and 163 (46%) landraces showed high partial resistance for early leaf and panicle blast, respectively,
with disease score of four to six. Field evaluation for leaf and panicle blast at three experimental sites in Bhutan showed
best cultivar discrimination in early leaf blast at the tillering stage (heritability, h2 = 0.32) and in the panicle blast at maturity (h2 = 0.44). Subdivision of the genetic variation into cultivar groups revealed the most variation for blast severity within
the 352 landraces with h2 of 0.31 and 0.46 for early leaf and panicle blast, respectively. Cluster analysis of the landraces revealed two distinct
rice cultivar groups, which separated at dissimilarity of 0.84 according to origin of the cultivars from low, mid and high
altitude zones in Bhutan. All microsatellites were polymorphic with two to 21 different alleles per marker and a high polymorphic
information content value of 0.61. The identified blast resistant landraces were genetically diverse originating from different
rice cultivation zones. Further investigation of the resistant and partial resistant material may reveal specific blast resistance
genes, which could be useful to mitigate blast incidence in rice-producing countries. 相似文献
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