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以原头节可溶性粗抗原经Sephadex G-200层析纯化抗原为包被抗原,兔抗羊IgG-HRP结合物为显色剂,建立检测羊脑多头蚴病血清抗体的Dot-ELISA,并以ELISA作平行对照。结果,粗抗原和层析纯化抗原检测86头份羊脑多头蚴病阳性血清,其敏感性分别为94.18%和93.02%,两种抗原的敏感性无显著差异;检测122头份绦虫蚴病阴性血清,18头份棘球蚴病阳性血清,35头份细颈囊尾蚴病阳性血清,其特异性分别为90.29%和95.43%。两种抗原的特异性差异显著。Dot-ELISA和ELISA两种方法的符合率为100%。层析纯化抗原比粗抗原的特异性有了明显提高,而敏感性没有降低。层析纯化抗原和操作术式都具有良好的重复性,Dot-ELISA和ELISA对比试验结果相近,且具简便、快速及不需要复杂设备等优点,是一种检测羊脑多头蚴病血清抗体的理想方法。  相似文献   
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Significant efforts should be devoted to reducing waste outputs from aquaculture operations in order to lower the environmental impacts of aquaculture in many parts of the world. Since most aquaculture wastes are ultimately from dietary origin, reduction of waste outputs should first be through improvements of diet formulation and feeding strategies. The first step in the production of feeds producing less solid waste is to eliminate poorly digestible ingredients (such as whole grain or grain by-products used as binders and fillers in the feed formulae) and to use highly digestible ingredients with good binding properties. Further reduction of solid waste can then be achieved through careful selection of the ingredients to improve apparent digestibility and the nutrient balance of the feed. Nitrogen waste outputs can be reduced through the reduction of the digestible protein to digestible energy (DP/DE) ratio of the diet. Phosphorus waste outputs can be reduced through careful selection of the ingredients and optimization of the digestible phosphorus content of the diet to meet the requirement of the fish but avoid greatly exceeding this required level. Finally, feeding practices that minimize feed wastage should be adopted since feed wastage can have a very significant impact on waste outputs from fish culture operations.  相似文献   
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山洪对房屋的破坏随着生态环境的恶化而加剧,其损失又随人民生活水平的提高而增加。防患房屋遭受山洪破坏刻不容缓。本文客观地总结了山洪破坏房屋具有的区域性、多样性、毁灭性特征,通过分析房屋遭受山洪破坏的沉痼陋习,初步揭示了山洪惩罚人为性大失误的沉痛教训,进而提出防患山洪破坏房屋要非工程措施与工程措施相结合,运用社会、经济、法律的手段,加以减灾引导,加强建房管理,采取开撇水沟、挖水平槽、刨鱼鳞坑、砌护坡墙、打挡水桩等直接有效的工程措施,加速隐患补救,实现减灾目的。  相似文献   
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调查研究结果表明,盐城市滨海盐土区与杉木中心产区的气候因子比较接近,基本适宜杉木的生长,杉木造林选择地下一在1.5m以下,排水良好的采伐更新迹地并采用 栽植法效果较好。新造幼林套种作物以棉花最好。  相似文献   
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1. The fear levels of broilers arriving from slaughter at 4 commercial processing plants were assessed using tonic immobility (TI) tests. The relative effects of selected variables were also examined.

2. Neither the position of the birds on the lorry, nor their sex, nor the ambient temperature nor the identity of the experimeter exerted any significant effects on tonic immobility reactions.

3. Although the longest and shortest immobility responses followed transportation in the fixed crates and metal modules respectively, system comparisons may be confounded by variations in the birds’ genetic and/or experiential backgrounds.

4. Both journey duration and waiting time before transport bore an unambiguous and positive linear relationship with mean TI duration. The duration of transit was the major factor determining fear levels following both commercial and simulated journeys.  相似文献   

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该文用层次分析法(AHP)和德尔菲法(Delphi)相结合的系统分析法,对江西省上饶地区小水电科技发展领域分析模型作了探索,提出了小水电科技发展领域的优先度及重点,为“八五”、“九五”小水电科技规划的制定提供了依据,同时也为水利水电同行在科技规划中提供一点参考。  相似文献   
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Engineering resistance against various diseases and pests is hampered by the lack of suitable genes. To overcome this problem we started a research program aimed at obtaining resistance by transfecting plants with genes encoding monoclonal antibodies against pathogen specific proteins. The idea is that monoclonal antibodies will inhibit the biological activity of molecules that are essential for the pathogenesis. Potato cyst nematodes are chosen as a model and it is thought that monoclonal antibodies are able to block the function of the saliva proteins of this parasite. These proteins are, among others, responsible for the induction of multinucleate transfer cells upon which the nematode feeds. It is well documented that the ability of antibodies to bind molecules is sufficient to inactivate the function of an antigen and in view of the potential of animals to synthesize antibodies to almost any molecular structure, this strategy should be feasible for a wide range of diseases and pests.Antibodies have several desirable features with regard to protein engineering. The antibody (IgG) is a Y-shaped molecule, in which the domains forming the tips of the arms bind to antigen and those forming the stem are responsible for triggering effector functions (Fc fragments) that eliminate the antigen from the animal. Domains carrying the antigen-binding loops (Fv and Fab fragments) can be used separately from the Fc fragments without loss of affinity. The antigen-binding domains can also be endowed with new properties by fusing them to toxins or enzymes. Antibody engineering is also facilitated by the Polymerase Chain Reaction (PCR). A systematic comparison of the nucleotide sequence of more than 100 antibodies revealed that not only the 3′-ends, but also the 5′-ends of the antibody genes are relatively conserved. We were able to design a small set of primers with restriction sites for forced cloning, which allowed the amplification of genes encoding antibodies specific for the saliva proteins ofGlobodera rostochiensis. Complete heavy and light chain genes as well as single chain Fv fragments (scFv), in which the variable parts of the light (VL) and heavy chain (VH) are linked by a peptide, will be transferred to potato plants. A major challenge will be to establish a correct expression of the antibody genes with regard to three dimensional folding, assembly and intracellular location.  相似文献   
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