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Nonhost resistance describes the immunity of an entire plant species against nonadapted pathogen species. We report that Arabidopsis PEN2 restricts pathogen entry of two ascomycete powdery mildew fungi that in nature colonize grass and pea species. The PEN2 glycosyl hydrolase localizes to peroxisomes and acts as a component of an inducible preinvasion resistance mechanism. Postinvasion fungal growth is blocked by a separate resistance layer requiring the EDS1-PAD4-SAG101 signaling complex, which is known to function in basal and resistance (R) gene-triggered immunity. Concurrent impairment of pre- and postinvasion resistance renders Arabidopsis a host for both nonadapted fungi.  相似文献   
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Autochthony in forest tree stands is characterized by a number of criteria, among which the range over which stands act as a population has been suggested to play a central role. Therefore, measures are needed for the delineation of populations or the detection of subpopulation structure. It is argued here that methods of population delineation must be based on the combined consideration of spatial distances and genetic differences between adult individuals. Conventional approaches and a set of newly developed methods are applied to seven isozyme loci in four beech stands which are distinguished by different types of forest management based on natural regeneration.  相似文献   
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The precipitation of dissolved organic matter (DOM) by aluminum (Al) results in a stable soil organic matter (OM) fraction. Extracellular enzymes can also be removed from soil solution by sorption or precipitation, but whether this affects their activity and their importance for carbon (C) mineralization is largely unknown. We studied the activity of eight extracellular enzymes, precipitated by Al together with DOM, in relation to C mineralization of the precipitated OM. Dissolved OM was obtained from the Oi and Oa horizon of two forest soils and precipitated at different Al : C ratios and pH values to achieve a large variation in composition and C mineralization of precipitated OM. All eight enzymes were present in a functional state in precipitated OM. On average 53% of DOM was precipitated, containing on average 17%–41% of the enzyme activity (EA) involved in C degradation (chitinase, cellobiohydrolase, β‐glucosidase, glucuronidase, lacasse, and xylosidase) previously present in soil solution. In contrast, on average only 4%–7% of leucine‐aminopeptidase and acid‐phosphatase activity was found in precipitated OM. The EA found in precipitates significantly increased the percentage of C mineralized of precipitated OM, with a stronger influence of C‐degrading enzymes than enzymes involved in N and P cycling. However, after 8 weeks of incubation the correlations between EA and C mineralization disappeared, despite substantial EA being still present and only 0.5%–7.7% of C mineralized. Thus, degradation of precipitated OM seems to be governed by EA during the first degradation phase, but the long‐term stability of precipitated OM is probably related to its chemical properties.  相似文献   
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Carbon mineralization in acidic forest soils can be retarded by large concentrations of aluminium (Al). However, it is still unclear whether Al reduces C mineralization by direct toxicity to microorganisms or by decreased bioavailability of organic matter (OM) because dissolved organic matter (DOM) is precipitated by Al. We conducted an incubation experiment (6 weeks) with two DOM solutions (40 mg C litre?1) derived from two acidic forests and possessing large differences in composition. Aluminium was added to the solutions in realistic ranges for acidic soils (1.6–24 mg Al litre?1) at pHs of 3.8 and 4.5, to achieve differences in Al speciation. We determined different Al species, including the potentially toxic Al3+, by Diffusive Gradients in Thin Films (DGT) to evaluate toxic effects on microorganisms. Precipitation of OM increased with larger amounts of added Al and higher pH, and we measured a larger fraction of dissolved ‘free’ Al at pH 3.8 than at pH 4.5. Organic matter degradation decreased significantly with Al addition, and we found more organic matter degraded at pH 3.8 than at pH 4.5 for the respective Al additions. Consequently, the observed reduction in OM degradation (i.e. stabilization) cannot be explained by toxic effects of ‘free’ Al. However, C stabilization correlated significantly with C precipitation. The pH did not influence C stabilization directly, but determined the amount of C being precipitated. Phosphorus was removed along with OM by precipitation, which possibly also affected C stabilization. We conclude that C stabilization upon Al addition did not result from toxic effects, but was caused by reduced bioavailability of OM after its precipitation. The reduction in OM degradation by 65% is of great relevance for the overall C stabilization in acidic forest soils. Increasing pH and decreasing Al concentrations upon recovery from acidic deposition should therefore not result in decreased stabilization of precipitated OM.  相似文献   
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