Specificities of antinuclear antibodies detected in dogs with systemic lupus erythematosus   总被引：4，自引：0，他引：4  

O Costa  C Fournel  E Lotchouang  J C Monier  M Fontaine 《Veterinary immunology and immunopathology》1984,7(3-4):369-382

Five hundred and eighty dogs with at least one clinical sign compatible with a systemic lupus erythematosus (SLE) were entered in a prospective study aimed at evaluating the prevalence of antinuclear antibodies (ANAb). SLE was diagnosed in 38 of these dogs (group A) which fulfilled at least four American Rheumatism Association (ARA) criteria; of these, sixteen had ANAb titers greater than or equal to 4096. The 23 dogs which met three or two ARA criteria (group B) had an ANAb geometric mean titer (GMT) of 259. Dogs (group C) with only 1 criterium had an ANAb GMT of 75. Anti-ds-DNA Ab were present in 6 dogs from group A (16%), and 2 dogs from group B (9%). Anti-histone Ab were present among dogs from group A, B and C with frequencies of 81%, 67% and 26%, respectively. Among dogs from group A, the ANAb titers and the levels of anti-histone Ab correlated positively when individual sera were considered. Antibodies against the soluble nuclear antigen (SNA) were detected in 74%, 39% and 13% of the dogs from groups A, B and C, respectively. Antibodies initially described in human SLE also exist in SLE dogs. Anti-Sm Ab were found in 24% of dogs in group A. With anti-RNP Ab the frequency was still lower (10%). However, two other types of anti-SNA Ab against RNAse and trypsin-resistant antigens, not found in human "reference sera", were often detected. The first type (anti-type 1 Ab) was found in 26% and 9% of group A and group B, The first type (anti-type 1 Ab) was found in 26% and 9% of group A and group B, respectively; the second type (anti-type 2 Ab) is less frequent, and was found in 13% and 17% of group A and B, respectively. It appears that testing for anti-Sm, anti-type 1 and anti-histone Ab should be performed in order to improve the diagnosis of SLE in dogs.  相似文献   

Fine needle aspiration biopsy of malignant tumors in dogs and cats: a report of 102 cases          下载免费PDF全文

Ménard M  Fontaine M  Morin M 《The Canadian veterinary journal. La revue veterinaire canadienne》1986,27(12):504-510

Fine needle aspiration biopsy is a fast and inexpensive technique well tolerated by animals. Its efficacy for the diagnosis of malignancy and determination of cellular origin was investigated for 97 malignant tumors and five benign lesions in 83 dogs and 19 cats. Histological examination of the lesions was performed in each case. Malignancy was detected by cytology in 69% of the malignant tumors. The cellular origin of the lesion was determined in 74% of the cases. Confirmation of malignancy and determination of cellular origin permitted an early diagnosis and prognosis. Since fine needle aspiration biopsy and cytology cannot definitely rule out malignancy, a surgical biopsy and histopathology should be done when malignancy is suspected clinically and not confirmed cytologically. Specimens of good quality, adequate collaboration between the cytologist and clinicians, and sufficient knowledge of pathology and cytology are basic requirements for obtaining good results with fine needle aspiration biopsy and cytology.  相似文献   

Analysis of pastoralists’ perception on challenges and opportunities for sheep and goat production in Northern Kenya     

Abdilatif  Mohamed Haji  Onono  Joshua Orungo  Mutua  Florence Kanini 《Tropical animal health and production》2018,50(7):1701-1710

Tropical Animal Health and Production - Small ruminants’ production contributes to livelihood of pastoral communities, but this faces myriad constraints. This study aimed at identifying...  相似文献   

Expression of interleukin-1beta in the digital laminae of horses in the prodromal stage of experimentally induced laminitis     

Fontaine GL  Belknap JK  Allen D  Moore JN  Kroll DL 《American journal of veterinary research》2001,62(5):714-720

OBJECTIVE: To study expression of interleukin-1beta (IL-1beta) in the digital laminae of horses in the prodromal stage of experimentally induced laminitis. ANIMALS: 8 healthy adult horses with no signs of laminitis. PROCEDURES: Black walnut extract was administered via nasogastric tube to 4 horses, and water was administered to the remaining 4 (controls). Complete blood counts and physical examinations were performed every 30 minutes after administration of black walnut extract or water. General anesthesia was induced when total WBC count decreased by 30% in horses given the black walnut extract and 3 hours after water administration in control horses. The left forefoot was perfusion fixed with neutral-buffered 10% formalin, and paraffin-embedded sections of the digit were used for in situ hybridization with an equine-specific IL-1beta probe. RESULTS: IL-1beta mRNA expression was observed in perivascular cells of the small laminar venules and capillaries in all 4 horses given black walnut extract and in interstitial cells remote from the microvasculature in 1 of the 4. Other cellular components of the laminar tissue and cellular components of the digital arterioles and veins did not exhibit IL-1beta mRNA expression. Expression of IL-1beta mRNA was not detected in laminae from control horses. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that IL-1beta mRNA is expressed by perivascular cells in the laminar tissues of horses in the prodromal stage of experimentally induced laminitis. This provides evidence of an inflammatory process during the prodromal stage of laminitis, indicating that local digital proinflammatory cytokine expression may be an initiating factor in laminitis.  相似文献   

Application of a putative fatty-acid binding protein to discriminate serologically the two European quarantine root-knot nematodes, Meloidogyne chitwoodi and M. fallax, from other Meloidogyne species     

Christophe Tastet  Florence Val  Michel Lesage  Lionel Renault  Laurent Marché  Michel Bossis  Didier Mugniéry 《European journal of plant pathology / European Foundation for Plant Pathology》2001,107(8):821-832

Two major proteins, Mcf-A67 and Mcf-B66, were identified by mini two-dimensional polyacrylamide gel electrophoresis in order to distinguish the two European quarantine root-knot nematodes, Meloidogyne chitwoodi and M. fallax, from eight other species. These quarantine proteinic markers have been microsequenced after enzymatic digestion. The internal amino acid sequences exhibit similarities to members of a family of low molecular weight intracellular lipid-binding proteins. Moreover, to explore a simple, rapid, and inexpensive way to identify the two quarantine nematodes, dot blot hybridizations were performed using an antiserum (A67) produced from the longest amino-acid sequence of the protein Mcf-A67. Although several proteins stained on the M. chitwoodi and M. fallax western blot membranes, the two nematodes were easily distinguished from other root-knot nematodes, on dot blot assays with soluble proteins extracted from a single female. Because of its specificity and sensitivity, the use of the A67 antiserum to improve the diagnosis of the two European quarantine root-knot nematodes is discussed.  相似文献   

Dilemma of virulence of Streptococcus suis: Canadian isolate 89-1591 characterized as a virulent strain using a standardized experimental model in pigs   总被引：4，自引：0，他引：4       下载免费PDF全文

Florence Berthelot-Hérault  Marcelo Gottschalk  Hervé Morvan    Marylène Kobisch 《Canadian journal of veterinary research》2005,69(3):236-240

Virulence of Streptococccus suis capsular type 2 strain 89-1591 has been controversial in literature. A standardized experimental model with specific-pathogen free piglets was used for a new evaluation of this strain. Twenty-nine piglets were allotted in 4 separated groups. Group 1 consisted of negative control animals which received broth medium. Groups 2, 3, and 4 were intravenously challenged with 2 mL of S. suis, strains 1330, 89-1591, and 166', respectively. The strain 1330 is a recognized avirulent Canadian strain. The strain 166' is a reference French virulent isolate. Pigs inoculated with strain 1330 did not present clinical signs of a S. suis infection. Contamination in organs and bacterial blood circulation were rare and lesions were almost non-existent. Infection of pigs with S. suis strain 89-1591 (group 3) and 166' (group 4) caused severe clinical problems, animals infected with S. suis 166' were the most affected. Pigs presented with clinical signs such as high body temperature, lameness, nervous symptoms, and even mortality. Lesions associated with S. suis were numerous for both strains, but more evident in animals of group 4. It can be concluded that S. suis strain 89-1591 is virulent, although its virulence seems to be lower than that of the French strain. Results of an experimental infection with strain 89-1591 may depend on different factors such as the route of inoculation and the immunological status of the animals used. Using conventional animals, with an unknown status regarding previous S. suis infections, equivocal results may be obtained, and this may explain differences reported by some authors with the same strain.  相似文献   

Inhibition of acetolactate synthase by sulfonylureas: Reversibility,inhibitor binding and mechanism     

Jean Bastide  Florence Ortega 《Pest management science》1995,43(3):253-255

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Evaluation of a rapid method of determination of plasma fibrinogen in swine.          下载免费PDF全文

M Fontaine  B J McSherry    V E Valli 《Canadian journal of veterinary research》1977,41(2):202-205

An evaluation was made of a rapid semiautomated method for determining fibrinogen level in swine plasma. This method, referred to as thrombin time method or fibrometer method, is based on the principle that when thrombin is added to suitably diluted plasma, the time of clotting is linearly related to the fibrinogen concentration. The linear regression model for the standard curve prepared using swine plasma had an r value of 0.998. A comparison between the fibrometer and the Grannis methods done on 189 swine plasma samples showed good correlation between these two mehtods (r value 0.847). It was concluded that although the fibrometer method may not be as precise as the Grannis method, it would still be acceptable for clinical use in swine.  相似文献   

Mechanisms of resistance to fungicides in field strains of Botrytis cinerea     

Leroux P  Fritz R  Debieu D  Albertini C  Lanen C  Bach J  Gredt M  Chapeland F 《Pest management science》2002,58(9):876-888

Field strains of Botrytis cinerea Pers ex Fr, the causal agent of grey mould diseases, were collected from French vineyards between 1993 and 2000. Several phenotypes have been characterized according to the inhibitory effects of fungicides towards germ-tube elongation and mycelial growth. Two types of benzimidazole-resistant strains (Ben R1 and Ben R2) could be detected; negative cross-resistance to phenylcarbamates (e.g. diethofencarb) was only found in Ben R1. Benzimidazole resistance was related to point mutations at codon 198 (Ben R1) or 200 (Ben R2) of the beta-tubulin gene. Most dicarboximide-resistant strains were also weakly resistant to aromatic hydrocarbon fungicides (e.g. dicloran) but remained sensitive to phenylpyrroles (e.g. fludioxonil). These resistant field strains (Imi R1) contained a single base pair mutation at position 365 in a two-component histidine kinase gene, probably involved in the fungal osmoregulation. Three anilinopyrimidine-resistant phenotypes have been identified. In the most resistant one (Ani R1), resistance was restricted to anilinopyrimidines, but no differences were observed in the amino-acid sequences of cystathionine beta-lyase (the potential target site of these fungicides) from Ani R1 or wild-type strains. In the two other phenotypes (Ani R2 and Ani R3), resistance extended to various other groups of fungicide, including dicarboximides, phenylpyrroles and sterol biosynthesis inhibitors. This multi-drug resistance was probably determined by over-production of ATP-binding cassette transporters. The hydroxyanilide fenhexamid is a novel botryticide whose primary target site is the 3-keto reductase involved in sterol C-4 demethylations. Apart from the multi-drug-resistant strain Ani R3, three other fenhexamid-resistant phenotypes have been recognized. For two of them (Hyd R1 and Hyd R2) fenhexamid-resistance seemed to result from P450-mediated detoxification. Reduced sensitivity of the target site could be the putative resistance mechanism operating in the third resistant phenotype (Hyd R3). Increased sensitivity to inhibitors of sterol 14 alpha-demethylase recorded in Hyd R1 strains was related to two amino-acid changes at positions 15 and 105 of this enzyme.  相似文献   

Cytotoxic constituents from Plumbago zeylanica   总被引：5，自引：0，他引：5  

Nguyen AT  Malonne H  Duez P  Vanhaelen-Fastre R  Vanhaelen M  Fontaine J 《Fitoterapia》2004,75(5):500-504

The bioassay-guided fractionation of the dichloromethane extract of aerial parts of Plumbago zeylanica led to the isolation of beta-sitosterol, beta-sitosteryl-3beta-glucopyranoside, beta-sitosteryl-3beta-glucopyranoside-6'-O-palmitate (1), lupenone, lupeol acetate, plumbagin and trilinolein. Compound 1 showed cytotoxic activity against MCF7 and Bowes cancer cell lines (IC50 113 microM and 152 microM, respectively), beta-sitosterol inhibited Bowes cell growth (IC50 36.5 microM) and plumbagin was cytotoxic against MCF7 and Bowes cells (IC50 1.28 microM and 1.39 microM, respectively).  相似文献