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1.
In order to offer hints for new research, the agronomic techniques used to manageOrobanche crenata in Sicily in the late 19 th and early 20 th Century are examined; the publications in which they were originally described can be obtained only with difficulty. Hand pulling, resistant cultivars and late sowing are the techniques used nowadays. Screening for resistance to broomrape of the traditional cultivars of broad bean, and for the influence of sowing density on broomrape infestations, is still to be done.  相似文献   
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The aim of this investigation was to study the effects of clenbuterol treatment (1 mug/kg b.w. i.m. for 10 consecutive days) on haemocoagulation process in seven calves. From each animal, blood samples were taken before (basal sample) and 24, 72, 144, 216, 264, 336 and 360 h after the end of treatment and some parameters were evaluated: coagulation process [prothrombin (PTT) and thrombin times (PT)], a complete blood count and plasmatic levels of 3'5'-monophosphatocyclic-adenosine (c-AMP) . A comparison of the values in each time group during the whole course of the experiments shows a significant increase (P < 0.03, P < 0.01) in PT and PTT at 72, 144, 216 and 264 h. Significant increase (P < 0.01) in c-AMP plasma levels were observed at 144, 216 and 264 h. All these values returned to normal levels after 360 h.  相似文献   
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Mal secco is a serious tracheomycosis, caused by the fungus Phoma tracheiphila (Petri) Kanc, et Ghik., which attacks some species of the genus Citrus. The considerable amount of time and space required to evaluate host plant response has discouraged research on the mechanism of inheritance involved in the susceptibility to this disease. The aim of the present work was to study segregation of the resistance trait in three progenies obtained by crossing Citrus latipes (Swing.) Tan., as a female parent, with the male parents Poncirus trifoliata (L.) Raf., C.aurantium L. and C. volkameriana Pasq. Resistance determination was based on natural infections observed over 10 years. Results allowed us to hypothesize three alternative genes (A, B and C) which determine dominant resistance as opposed to susceptibility: the presence of a single allele can thus confer resistance. In addition, we suggest the existence of a fourth gene which, in conditions of dominance, is able to determine susceptibility by nullifying the action of the dominant B allele. Response to the pathogen was also associated with the production of chitinase a PR (pathogenesis-related) protein reported to be present in great quantities in mal secco-resistant Citrus genotypes. Segregation analysis of one F2 progeny confirmed the hypothesis that the resistance trait is controlled by at least two dominant genes. Further evidence is needed to ascertain whether this hypothesis is applicable to other Citrus species, in which the possibility of other genetic mechanisms being implicated cannot be ruled out, thus making the genetic basis of mal secco resisance more complex.  相似文献   
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<正>Dear Editor,Soil quality is defined as“the capacity of a soil to function,within ecosystem and land use boundaries,to sustain productivity,maintain environmental quality,and promote plant and animal health”(Doran and Parkin 1994).In this context,soil functionality is fundamental to the biosphere and is highly dependent on the associated microbiota.Soil microorganisms are relevant for terrestrial ecosystem functioning,and understanding the functional changes of the biological community i...  相似文献   
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We investigated the influence of monoammonium phosphate (MAP) and diammonium phosphate (DAP) on the adsorption process of Cd by two typic soils. The phosphate induced variations in the pH values of soil-Cd suspensions, drastically influenced the solubility of the heavy metal in the two soils. MAP decreased the amount adsorbed while DAP increased the quantity fixed by the soil. The effect was particularly remarkable in the soil with low content of organic matter (Lamporecchio soil) where the addition of DAP caused the precipitation of the heavy metal. In the organic soil (Orentano soil) the effect of phosphate was less evident because of the high buffering capacity of this soil, rich in colloidal fractions.  相似文献   
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Purpose

The possibility of using chemical and microbial additives to enhance the phytoextraction of mercury (Hg) and arsenic (As) from a multi-contaminated soil could be very effective, leading to a significant saving in terms of time and costs of the reclamation. The aim of this study was to evaluate the efficacy of the addition of (i) thiosulfate and (ii) metal-tolerant bacteria isolated from the polluted soil having plant growth promotion (PGP) potential to perform As and Hg phytoextraction by Brassica juncea and Lupinus albus.

Materials and methods

A collection of 13 bacterial isolates able to tolerate As and Hg was obtained from the contaminated soil, identified by partial 16S rRNA gene sequencing and tested in vitro for PGP activities. The most promising strains were further tested in vivo for the evaluation of plant growth ability and rhizocompetence on model plants. Pot experiments were conducted in microcosms, with polluted soil vegetated with B. juncea and L. albus. Ammonium thiosulfate and potassium dihydrogen phosphate were used as mobilizing agents, together with a bacterial consortium composed by the most promising PGP isolates.

Results and discussion

Thirteen indigenous metal-tolerant bacterial strains were isolated, and their in vitro characterization highlighted their great potential in assisting the phytoremediation process; most of them tolerated both trace elements and showed, at the same time, multiple PGP traits. The results were confirmed in vivo on model plants and lead to the selection of the most promising PGP strains to be applied in microcosm-scale phytoextraction experiments. Thiosulfate addition significantly increased the mobilization of both elements, promoting bioavailability and phytoextraction. When a selected bacterial consortium was supplemented in addition to thiosulfate, the efficacy of the phytoaccumulation was increased up to 85 % for As and up to 45 % for Hg.

Conclusions

The use of the common fertilizer thiosulfate appeared to have great potential in phytoextraction practices since it was able to facilitate the uptake by plants of both Hg and As. Moreover, the application of a consortium of indigenous PGP bacteria (PGPB) produced a further positive effect on the plant biomass, supporting and enhancing the phytoextraction strategy, thus demonstrating their potential in a microbe-assisted phytoremediation intervention.
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Soybean seedlings (Glycine max) were exposed to simulated acid rain containing sulfate ion only or a mixture of sulfate, nitrate and chloride anions, using a continuous rain generating system in a side opened glasshouse. Plants were subjected to acid rain treatment twice a week, for a 1 or 3 hr period at a rate of 2.2 or 5.0 mm hr?1, respectively. Dry seed yield in plants treated with simulated acid rain at pH 2.0, in the three of 4 experiments conducted over a 3 yr period, was significantly less than that at pH 3.0 or higher. Simulated acid rain treatment at pH 3.0 or higher did not significantly affect yield compared to pH 5.6; however, plants exposed to simulated acid rain at pH 4.0 tended to yield more than those treated with pH 5.6 rain. Based on the current 3 years of research in which results from 4 experiments were combined, rain acidity at current levels in Japan would not directly affect seed production of selected cultivars of soybean.  相似文献   
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The Authors describe chronic vaginitis of bovines in intensive breeding. All of the 78 subjects examined presented vaginitis, accompanied by fibrinous hydrocele. On the spermatic cord and the tail of epididymis, initial lesions consisted of granulomas and fibrinous bridges between vaginalis propria of testicle and of epididymis. Subsequently appeared adherence and synechiae, which join the deferens and epididymis body to didymis, and thickening of albuginea. Synechiae can develop between peritesticular vaginalis propria and communis. Histopathological findings: peritoneal-like papillary structures with metaplastic mesothelial cells; diffuse lympho-plasmacellular infiltration with eosinophils and mast cells; intense capillary angiogenesis. The epididymal lesions are: diffuse mononuclear infiltration; periarteritis nodosa; papillary proliferations of endothelium; endothelial degeneration; tubular ectasia and stenosis; intraepithelial cyst. Noticeable within testis: periarteritis nodosa; fibrosis of albuginea; spermatocytes vacuolisation; multinucleate spermatids; epithelial shedding. The Authors pointed out: the serological and bacteriological testings furnished inconclusive results; only pathological finding always present is a large infestation of cremasteric muscle with Sarcocystis, to which is attributed the etiopathogenesis of flogosis, that is chronic from the beginning. The Authors propose the terminology of "Chronic peritesticular and periepididymal vaginitis" or "Chronic Periorchitis and periepididymitis".  相似文献   
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